Construct: ORF TRCN0000467676
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008094.1_s317c1
- Derived from:
- ccsbBroadEn_10821
- DNA Barcode:
- TCACACTCCGTCTTATAAACCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGF7 (2252)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467676
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2252 | FGF7 | fibroblast growth factor 7 | NM_002009.4 | 49.6% | 48.4% | (many diffs) |
| 2 | mouse | 14178 | Fgf7 | fibroblast growth factor 7 | NM_008008.4 | 44.5% | 44.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 381
- ORF length:
- 291
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCACAAATG GATACTGACA TGGATCCTGC 121 CAACTTTGCT CTACAGATCA TGCTTTCACA TTATCTGTCT AGTGGGTACT ATATCTTTAG 181 CTTGCAATGA CATGACTCCA GAGCAAATGG CTACAAATGT GAACTGTTCC AGCCCTGAGC 241 GACACACAAG AAGTTATGAT TACATGGAAG GAGAGGATAT AAGAGTGAGA AGACTCTTCT 301 GTCGAACACA GTGGTACCTG AGGATCGATA AAAGAGGCAA AGTAAAAGGG ACCCAAGAGA 361 TGAAGAATAA TTACAGTAAG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 421 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 481 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCAC ACTCCGTCTT 541 ATAAACCGCG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt