Construct: ORF TRCN0000467726
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008544.1_s317c1
- Derived from:
- BRDN0000393355
- DNA Barcode:
- CGACGCTTGCAGTCTCACCATATG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- OGT (8473)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467726
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8473 | OGT | O-linked N-acetylglucosamin... | NM_181673.3 | 99.8% | .6% | 18_19insAA;22G>N;2549T>C |
| 2 | human | 8473 | OGT | O-linked N-acetylglucosamin... | NM_181672.3 | 98.9% | .5% | (many diffs) |
| 3 | human | 8473 | OGT | O-linked N-acetylglucosamin... | XM_017029908.1 | 64.1% | .7% | 0_1ins1115;1436T>C |
| 4 | human | 8473 | OGT | O-linked N-acetylglucosamin... | XM_024452467.1 | 64.1% | .7% | 0_1ins1115;1436T>C |
| 5 | mouse | 108155 | Ogt | O-linked N-acetylglucosamin... | NM_001290535.1 | 92.2% | .6% | (many diffs) |
| 6 | mouse | 108155 | Ogt | O-linked N-acetylglucosamin... | XM_011247503.2 | 92.2% | .6% | (many diffs) |
| 7 | mouse | 108155 | Ogt | O-linked N-acetylglucosamin... | NM_139144.4 | 91.3% | .5% | (many diffs) |
| 8 | mouse | 108155 | Ogt | O-linked N-acetylglucosamin... | XM_006527736.3 | 91.3% | .5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 108
- ORF length:
- 42
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcttccgtg ggcaaaacnt ggccgacagc acagggttag ctgagttggc 121 acatcgagaa tatcaggcag gagattttga ggcagctgag agacactgca tgcagctctg 181 gagacaagag ccagacaata ctggtgtgct tttattactt tcatctatac acttccagtg 241 tcgaaggctg gacagatctg ctcactttag cactctggca attaaacaga acccccttct 301 ggcagaagct tattcgaatt tggggaatgt gtacaaggaa agagggcagt tgcaggaggc 361 aattgagcat tatcgacatg cattgcgtct caaacctgat ttcatcgatg gttatattaa 421 cctggcagcc gccttggtag cagcgggtga catggaaggg gcagtacaag cttacgtctc 481 tgctcttcag tacaatcctg atttgtactg tgttcgcagt gacctgggga acctgctcaa 541 agccctgggt cgcttggaag aagccaaggc atgttatttg aaagcaattg agacgcaacc 601 gaactttgca gtagcttgga gtaatcttgg ctgtgttttc aatgcacaag gggaaatttg 661 gcttgcaatt catcactttg aaaaggctgt cacccttgac ccaaactttc tggatgctta 721 tatcaattta ggaaatgtct tgaaagaggc acgcattttt gacagagctg tggcagctta 781 tcttcgtgcc ctaagtttga gtccaaatca cgcagtggtg cacggcaacc tggcttgtgt 841 atactatgag caaggcctga tagatctggc aatagacacc tacaggcggg ctatcgaact 901 acaaccacat ttccctgatg cttactgcaa cctagccaat gctctcaaag agaagggcag 961 tgttgctgaa gcagaagatt gttataatac agctctccgt ctgtgtccca cccatgcaga 1021 ctctctgaat aacctagcca atatcaaacg agaacaggga aacattgaag aggcagttcg 1081 cttgtatcgt aaagcattag aagtcttccc agagtttgct gctgcccatt caaatttagc 1141 aagtgtactg cagcagcagg gaaaactgca ggaagctctg atgcattata aggaggctat 1201 tcgaatcagt cctacctttg ctgatgccta ctctaatatg ggaaacactc taaaggagat 1261 gcaggatgtt cagggagcct tgcagtgtta tacgcgtgcc atccaaatta atcctgcatt 1321 tgcagatgca catagcaatc tggcttccat tcataaggat tcagggaata ttccagaagc 1381 catagcttct taccgcacgg ctctgaaact taagcctgat tttcctgatg cttattgtaa 1441 cttggctcat tgcctgcaga ttgtctgtga ttggacagac tatgatgagc gaatgaagaa 1501 gttggtcagt attgtggctg accagttaga gaagaatagg ttgccttctg tgcatcctca 1561 tcatagtatg ctatatcctc tttctcatgg cttcaggaag gctattgctg agaggcacgg 1621 caacctgtgc ttagataaga ttaatgttct tcataaacca ccatatgaac atccaaaaga 1681 cttgaagctc agtgatggtc ggctgcgtgt aggatatgtg agttccgact ttgggaatca 1741 tcctacttct caccttatgc agtctattcc aggcatgcac aatcctgata aatttgaggt 1801 gttctgttat gccctgagcc cagacgatgg cacaaacttc cgagtgaagg tgatggcaga 1861 agccaatcat ttcattgatc tttctcagat tccatgcaat ggaaaagcag ctgatcgcat 1921 ccatcaggat ggaattcata tccttgtaaa tatgaatggc tatactaagg gcgctcgaaa 1981 tgagcttttt gctctcaggc cagctcctat tcaggcaatg tggctgggat accctgggac 2041 gagtggtgcg cttttcatgg attatattat cactgatcag gaaacttcgc cagctgaagt 2101 tgctgagcag tattccgaga aattggctta tatgccccac acttttttta ttggtgatca 2161 tgctaatatg ttccctcacc tgaagaaaaa agcagtcatc gattttaagt ccaatgggca 2221 catttatgac aatcggatag ttctgaatgg catcgacctc aaagcatttc ttgatagtct 2281 accagatgtg aaaattgtca agatgaagtg tcctgatgga ggagacaatg cagatagcag 2341 taacacagct cttaatatgc ctgttattcc tatgaatact attgcagaag cagttattga 2401 aatgattaac cgaggacaga ttcaaataac aattaatgga ttcagtatta gcaatggact 2461 ggcaactact cagatcaaca ataaggctgc aactggagag gaggttcccc gtaccattat 2521 tgtaaccacc cgttctcagt acgggttacc agaagatgcc atcgtatact gtaactttaa 2581 tcagttgtat aaaattgacc cttctacttt gcagacgtgg gcaaacattc tgaagcgtgt 2641 tcccaatagt gtactctggc tgttgcgttt tccagcagta ggagaaccta atattcaaca 2701 gtatgcacaa aacatgggcc tgccccagaa ccgtatcatt ttttcacctg ttgctccTAA 2761 AGAGGAACAC GTCAGGAGAG GCCAGCTGGC TGATGTCTGC TTGGACACTC CACTCTGTAA 2821 TGGGCACACC ACAGGGATGG ATGTCCTCTG GGCAGGGACC CCCATGGTGA CTATGCCAGG 2881 AGAGACTCTT GCTTCTCGAG TTGCAGCATC CCAGCTCACT TGCTTAGGTT GTCTTGAGCT 2941 TATTGCTAAA AACAGACAAG AATATGAAGA CATAGCTGTG AAGCTGGGAA CTGATCTAGA 3001 ATACCTGAAG AAAGTTCGTG GCAAAGTCTG GAAGCAAAGA ATATCTAGCC CTCTGTTCAA 3061 CACCAAACAA TACACAATGG AACTAGAGCG GCTCTATCTA CAGATGTGGG AGCATTATGC 3121 AGCTGGCAAC AAACCTGACC ACATGATTAA GCCTGTTGAA GTCACTGAGT CAGCATACCC 3181 AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT 3241 CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA 3301 TATATCTTGT GGAAAGGACG ACGACGCTTG CAGTCTCACC ATATGACGCG TTAAGTCgac 3361 aatcaacctc tggattacaa aatttgtgaa agatt