Construct: ORF TRCN0000467739
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002908.1_s317c1
- Derived from:
- ccsbBroadEn_13695
- DNA Barcode:
- AGTTTAGGAATAACAATACATCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTHFD2L (441024)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467739
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NM_001351314.2 | 31.8% | 31.8% | 1_450del |
| 2 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NM_001004346.4 | 24.2% | 24.2% | 1_657del |
| 3 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NM_001144978.2 | 20.1% | 20.1% | 1_831del |
| 4 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_005265690.2 | 16.3% | 11% | 1_657del;758_831del;885_886ins56 |
| 5 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008225.1 | 16.3% | 11% | 1_657del;758_831del;885_886ins56 |
| 6 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008226.1 | 16.3% | 11% | 1_657del;758_831del;885_886ins56 |
| 7 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008224.1 | 14.8% | 10% | 1_753del;854_927del;981_982ins56 |
| 8 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008218.2 | 10.8% | 7.3% | 1_1140del;1241_1314del;1368_1369ins56 |
| 9 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NR_147097.2 | 8.4% | 1_984del;1195_2487del | |
| 10 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XR_001741222.2 | 7.4% | 1_1330del;1541_2833del | |
| 11 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XR_001741224.2 | 7.3% | 1_1336del;1547_2839del | |
| 12 | mouse | 665563 | Mthfd2l | methylenetetrahydrofolate d... | XM_006535178.3 | 21.5% | 22.9% | (many diffs) |
| 13 | mouse | 665563 | Mthfd2l | methylenetetrahydrofolate d... | NM_026788.1 | 17.7% | 18.9% | (many diffs) |
| 14 | mouse | 665563 | Mthfd2l | methylenetetrahydrofolate d... | XM_006535176.3 | 16.3% | 17.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 300
- ORF length:
- 210
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGTTAAAGA AGGTGCTGCT GTAATTGATG 121 TGGGTATCAA CTATGTCCAC GATCCAGTGA CAGGAAAGAC AAAATTAGTT GGAGATGTGG 181 ACTTCGAAGC TGTTAAAAAG AAAGCTGGCT TTATCACTCC AGTTCCAGGA GGTGTGGGAC 241 CCATGACAGT GGCAATGCTT CTGAAGAACA CCCTTCTGGC AGCTAAAAAA ATCATTTACT 301 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 361 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 421 TTTATATATC TTGTGGAAAG GACGAAGTTT AGGAATAACA ATACATCTCA CGCGTTAAGT 481 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt