Construct: ORF TRCN0000467772
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017587.1_s317c1
- Derived from:
- ccsbBroadEn_10940
- DNA Barcode:
- ACAAATATGTCAAGAGTTCTGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT8 (3856)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467772
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3856 | KRT8 | keratin 8 | NM_001256293.2 | 57.7% | 57.7% | 1_612del |
| 2 | human | 3856 | KRT8 | keratin 8 | NM_002273.4 | 57.7% | 57.7% | 1_612del |
| 3 | human | 3856 | KRT8 | keratin 8 | NM_001256282.2 | 54.5% | 54.5% | 1_696del |
| 4 | human | 283102 | KRT8P41 | keratin 8 pseudogene 41 | NR_027713.1 | 40.5% | (many diffs) | |
| 5 | human | 3856 | KRT8 | keratin 8 | NR_045962.2 | 39.1% | 1_1063del;1901_2140del | |
| 6 | mouse | 16691 | Krt8 | keratin 8 | NM_031170.2 | 49.1% | 50.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 903
- ORF length:
- 837
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa caaggtagag ctggagtctc gcctggaagg gctgaccgac gagatcaact 121 tcctcaggca gctatatgaa gaggagatcc gggagctgca gtcccagatc tcggacacat 181 ctgtggtgct gtccatggac aacagccgct ccctggacat ggacagcatc attgctgagg 241 tcaaggcaca gtacgaggat attgccaacc gcagccgggc tgaggctgag agcatgtacc 301 agatcaagta tgaggagctg cagagcctgg ctgggaagca cggggatgac ctgcggcgca 361 caaagactga gatctctgag atgaaccgga acatcagccg gctccaggct gagattgagg 421 gcctcaaagg ccagagggct tccctggagg ccgccattgc agatgccgag cagcgtggag 481 agctggccat taaggatgcc aacgccaagt tgtccgagct ggaggccgcc ctgcagcggg 541 ccaagcagga catggcgcgg cagctgcgtg agtaccagga gctgatgaac gtcaagctgg 601 ccctggacat cgagatcgcc acctacagga agctgctgga gggcgaggag agccggctgg 661 agtctgggat gcagaacatg agtattcata cgaagaccac cagcggctat gcaggtggtc 721 tgagctcggc ctatgggggc ctcacaagcc ccggcctcag ctacagcctg ggctccagct 781 ttggctcTGG CGCGGGCTCC AGCTCCTTCA GCCGCACCAG CTCCTCCAGG GCCGTGGTTG 841 TGAAGAAGAT CGAGACACGT GATGGGAAGC TGGTGTCTGA GTCCTCTGAC GTCCTGCCCA 901 AGTGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 961 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1021 GGCTTTATAT ATCTTGTGGA AAGGACGAAC AAATATGTCA AGAGTTCTGA CCACGCGTTA 1081 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt