Construct: ORF TRCN0000467775

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013212.1_s317c1
Derived from:
ccsbBroadEn_08019
DNA Barcode:
AATGTCAATCCCCGATGTAATACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NARF (26502)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467775
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26502 NARF nuclear prelamin A recognit... NM_031968.2 99.9% 99.8% 1442G>A
2 human 26502 NARF nuclear prelamin A recognit... NM_012336.4 90.7% 90.6% 767_768ins138;1304G>A
3 human 26502 NARF nuclear prelamin A recognit... XM_006722278.4 81.8% 81.6% 383_384ins135;632_633ins138;1169G>A
4 human 26502 NARF nuclear prelamin A recognit... NM_001083608.2 81.2% 81% 106_107ins144;623_624ins138;1160G>A
5 human 26502 NARF nuclear prelamin A recognit... NM_001038618.3 79% 78.8% 0_1ins177;590_591ins138;1127G>A
6 human 26502 NARF nuclear prelamin A recognit... XM_005256340.4 79% 78.8% 0_1ins177;590_591ins138;1127G>A
7 human 26502 NARF nuclear prelamin A recognit... XM_011523541.2 79% 78.8% 0_1ins177;590_591ins138;1127G>A
8 human 26502 NARF nuclear prelamin A recognit... XM_017024449.2 52% 50.2% (many diffs)
9 human 26502 NARF nuclear prelamin A recognit... XM_011523543.3 50.9% 50.7% 0_1ins600;167_168ins138;704G>A
10 human 26502 NARF nuclear prelamin A recognit... XM_017024450.2 50.9% 50.7% 0_1ins600;167_168ins138;704G>A
11 human 26502 NARF nuclear prelamin A recognit... XM_011523544.2 48% 42.8% (many diffs)
12 mouse 67608 Narf nuclear prelamin A recognit... NM_026272.3 74.9% 76.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1572
ORF length:
1506
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttngcatgaa gtgtgagcac tgcacgcgca aggaatgtag taagaaaaca aaaactgatg
121accaagagaa tgtgtcagcc gatgcaccga gtccagccca ggaaaatgga gagaagggag
181aattccacaa gttggctgat gccaagatat ttttgagcga ctgcctggca tgtgacagct
241gtatgactgc agaggaagga gtccaacttt cccagcaaaa tgccaaggac ttcttccgcg
301ttctgaacct taacaagaaa tgtgatacct caaagcacaa agtgctggta gtgtctgtgt
361gtcctcaatc tttgccttat tttgctgcta aattcaacct cagtgtaact gatgcatcca
421gaagactctg tggtttcctc aaaagtcttg gggtgcacta tgtatttgat acgacgatag
481ctgcggattt tagtatcctg gagagtcaaa aagaattcgt gcgtcgctat cgccagcaca
541gtgaggagga acgcaccctg cccatgctga cctctgcctg tcctggctgg gtccgatacg
601ccgagcgggt gctgggtcgc cccatcactg cccacctctg caccgccaag tccccccagc
661aggtcatggg ctctttggtg aaggattatt tcgccagaca gcagaacctg tctccagaga
721agattttcca cgtcattgtg gccccttgtt atgacaagaa gctggaggct cttcaggaaa
781gccttccccc tgctttgcat ggctcccggg gcgctgactg cgtgttaaca tcagaaatta
841gccaggcgtg gtggtgcaca cctgtgatca cagctactcg ggaggctgcg gcaagagaat
901cacttgaacc cgggaggcag aggttacagc gagacaagat tgcaccactg gactccagcc
961tgggcggcgg aggtgaaatt gctcaaataa tggagcaagg tgacctctca gtgagagatg
1021ctgccgtcga cactctgttt ggagacttga aggaggacaa agtgacgcgt catgatggag
1081ccagctcaga cgggcacctg gcacacatct tcagacatgc ggccaaggag ctgttcaacg
1141aggatgtgga ggaggtcact taccgagccc tgagaaacaa agacttccaa gaggtcaccc
1201ttGAGAAGAA CGGAGAGGTG GTGTTACGCT TTGCTGCAGC CTATGGCTTT CGAAACATCC
1261AGAACATGAT CCTGAAGCTT AAGAAGGGCA AGTTCCCATT CCACTTTGTG GAGGTCCTCG
1321CCTGTGCTGG AGGATGCTTA AATGGCAGAG GCCAAGCCCA GACTCCAGAC GGACATGCGG
1381ATAAGGCCCT GCTGCGGCAG ATGGAAGGCA TTTACGCTGA CATCCCTGTG CGGCGTCCGG
1441AGTCCAGTGC ACACGTGCAG GAGCTGTACC AGGAGTGGCT GGAGGGGATC AACTCCCCCA
1501AGGCCCAAGA GGTGCTGCAT ACCACGTACC AGAGCCAGGA GCGTGGCACA CACAGCCTGG
1561ACATCAAGTG GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA
1621ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT
1681CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAT GTCAATCCCC GATGTAATAC
1741GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)