Construct: ORF TRCN0000467775
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013212.1_s317c1
- Derived from:
- ccsbBroadEn_08019
- DNA Barcode:
- AATGTCAATCCCCGATGTAATACG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NARF (26502)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467775
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 26502 | NARF | nuclear prelamin A recognit... | NM_031968.2 | 99.9% | 99.8% | 1442G>A |
| 2 | human | 26502 | NARF | nuclear prelamin A recognit... | NM_012336.4 | 90.7% | 90.6% | 767_768ins138;1304G>A |
| 3 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_006722278.4 | 81.8% | 81.6% | 383_384ins135;632_633ins138;1169G>A |
| 4 | human | 26502 | NARF | nuclear prelamin A recognit... | NM_001083608.2 | 81.2% | 81% | 106_107ins144;623_624ins138;1160G>A |
| 5 | human | 26502 | NARF | nuclear prelamin A recognit... | NM_001038618.3 | 79% | 78.8% | 0_1ins177;590_591ins138;1127G>A |
| 6 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_005256340.4 | 79% | 78.8% | 0_1ins177;590_591ins138;1127G>A |
| 7 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_011523541.2 | 79% | 78.8% | 0_1ins177;590_591ins138;1127G>A |
| 8 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_017024449.2 | 52% | 50.2% | (many diffs) |
| 9 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_011523543.3 | 50.9% | 50.7% | 0_1ins600;167_168ins138;704G>A |
| 10 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_017024450.2 | 50.9% | 50.7% | 0_1ins600;167_168ins138;704G>A |
| 11 | human | 26502 | NARF | nuclear prelamin A recognit... | XM_011523544.2 | 48% | 42.8% | (many diffs) |
| 12 | mouse | 67608 | Narf | nuclear prelamin A recognit... | NM_026272.3 | 74.9% | 76.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1572
- ORF length:
- 1506
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttngcatgaa gtgtgagcac tgcacgcgca aggaatgtag taagaaaaca aaaactgatg 121 accaagagaa tgtgtcagcc gatgcaccga gtccagccca ggaaaatgga gagaagggag 181 aattccacaa gttggctgat gccaagatat ttttgagcga ctgcctggca tgtgacagct 241 gtatgactgc agaggaagga gtccaacttt cccagcaaaa tgccaaggac ttcttccgcg 301 ttctgaacct taacaagaaa tgtgatacct caaagcacaa agtgctggta gtgtctgtgt 361 gtcctcaatc tttgccttat tttgctgcta aattcaacct cagtgtaact gatgcatcca 421 gaagactctg tggtttcctc aaaagtcttg gggtgcacta tgtatttgat acgacgatag 481 ctgcggattt tagtatcctg gagagtcaaa aagaattcgt gcgtcgctat cgccagcaca 541 gtgaggagga acgcaccctg cccatgctga cctctgcctg tcctggctgg gtccgatacg 601 ccgagcgggt gctgggtcgc cccatcactg cccacctctg caccgccaag tccccccagc 661 aggtcatggg ctctttggtg aaggattatt tcgccagaca gcagaacctg tctccagaga 721 agattttcca cgtcattgtg gccccttgtt atgacaagaa gctggaggct cttcaggaaa 781 gccttccccc tgctttgcat ggctcccggg gcgctgactg cgtgttaaca tcagaaatta 841 gccaggcgtg gtggtgcaca cctgtgatca cagctactcg ggaggctgcg gcaagagaat 901 cacttgaacc cgggaggcag aggttacagc gagacaagat tgcaccactg gactccagcc 961 tgggcggcgg aggtgaaatt gctcaaataa tggagcaagg tgacctctca gtgagagatg 1021 ctgccgtcga cactctgttt ggagacttga aggaggacaa agtgacgcgt catgatggag 1081 ccagctcaga cgggcacctg gcacacatct tcagacatgc ggccaaggag ctgttcaacg 1141 aggatgtgga ggaggtcact taccgagccc tgagaaacaa agacttccaa gaggtcaccc 1201 ttGAGAAGAA CGGAGAGGTG GTGTTACGCT TTGCTGCAGC CTATGGCTTT CGAAACATCC 1261 AGAACATGAT CCTGAAGCTT AAGAAGGGCA AGTTCCCATT CCACTTTGTG GAGGTCCTCG 1321 CCTGTGCTGG AGGATGCTTA AATGGCAGAG GCCAAGCCCA GACTCCAGAC GGACATGCGG 1381 ATAAGGCCCT GCTGCGGCAG ATGGAAGGCA TTTACGCTGA CATCCCTGTG CGGCGTCCGG 1441 AGTCCAGTGC ACACGTGCAG GAGCTGTACC AGGAGTGGCT GGAGGGGATC AACTCCCCCA 1501 AGGCCCAAGA GGTGCTGCAT ACCACGTACC AGAGCCAGGA GCGTGGCACA CACAGCCTGG 1561 ACATCAAGTG GTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1621 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1681 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAAAT GTCAATCCCC GATGTAATAC 1741 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t