Construct: ORF TRCN0000467776

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005152.1_s317c1
Derived from:
ccsbBroadEn_13849
DNA Barcode:
TCATTTAGTGTGGCCCTGCCGTAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
EIF2S3 (1968)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467776
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1968 EIF2S3 eukaryotic translation init... NM_001415.4 99.8% 98.3% 588A>T;1398delG
2 human 255308 EIF2S3B eukaryotic translation init... NM_001357734.1 98% 96.8% (many diffs)
3 human 255308 EIF2S3B eukaryotic translation init... NM_001357731.1 92.6% 91.5% (many diffs)
4 mouse 26905 Eif2s3x eukaryotic translation init... NM_012010.3 90.7% 97.8% (many diffs)
5 mouse 26908 Eif2s3y eukaryotic translation init... NM_012011.1 89.1% 96.6% (many diffs)
6 mouse 26908 Eif2s3y eukaryotic translation init... XM_006531608.3 85.7% 93.6% (many diffs)
7 mouse 26908 Eif2s3y eukaryotic translation init... XM_011248593.1 85.7% 93.6% (many diffs)
8 mouse 26908 Eif2s3y eukaryotic translation init... XR_879402.2 75.4% (many diffs)
9 mouse 26908 Eif2s3y eukaryotic translation init... XR_001782886.1 66.2% (many diffs)
10 mouse 26908 Eif2s3y eukaryotic translation init... XR_001782885.1 66% (many diffs)
11 mouse 26908 Eif2s3y eukaryotic translation init... XR_388245.1 61.8% (many diffs)
12 mouse 26908 Eif2s3y eukaryotic translation init... XR_001782884.1 58% (many diffs)
13 mouse 26908 Eif2s3y eukaryotic translation init... XM_006531609.1 55.3% 59.9% (many diffs)
14 mouse 26908 Eif2s3y eukaryotic translation init... XM_006531610.3 55.3% 59.9% (many diffs)
15 mouse 26908 Eif2s3y eukaryotic translation init... XR_001782883.1 54.8% (many diffs)
16 mouse 26908 Eif2s3y eukaryotic translation init... XR_001782887.1 51.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1470
ORF length:
1404
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gggcggagaa gctggagtga ctctagggca gccgcatctt tcgcgtcagg
121atctcaccac cttggatgtt accaagttga cgccactttc acacgaagtt atcagcagac
181aagccacaat taacataggt acaattggtc atgtagctca tgggaaatcc acagtcgtca
241aagctatttc tggagttcat actgtcaggt tcaaaaatga actagaaaga aatattacaa
301tcaagcttgg atatgctaat gctaagattt ataagcttga tgacccaagt tgccctcggc
361cagaatgtta tagatcttgt gggagcagta cacctgacga gtttcctacg gacattccag
421ggaccaaagg gaacttcaaa ttagtcagac atgtttcctt tgttgactgt cctggccacg
481atattttgat ggctactatg ctgaacggtg cagcagtgat ggatgcagct cttctgttga
541tagctggtaa tgaatcttgc cctcagcctc agacatcgga acacctggct gctatagaga
601tcatgaaact gaagcatatt ttgattctac aaaataaaat tgatttggta aatgaaagtc
661aggctaaaga acaatacgag cagatccttg catttgtcca aggtacagta gcagagggag
721ctcccattat tccaatttca gctcagctga aatacaatat tgaagttgtt tgtgagtaca
781tagtaaagaa aattccagta cccccaagag actttacttc agagccccgg cttattgtta
841ttagatcttt tgatgtcaac aaacctggct gtgaagttga tgaccttaag ggaggtgtag
901ctggtggtag tatcctaaaa ggagtattaa aggtgggcca ggagatagaa gtaagacctg
961gtattgtttc caaagatagt gaaggaaaac tcatgtgtaa accaaTCTTT TCCAAAATTG
1021TATCACTTTT TGCGGAGCAT AATGATCTGC AATATGCTGC TCCAGGCGGT CTTATTGGAG
1081TTGGAACAAA AATTGACCCC ACTTTGTGCC GGGCTGACAG AATGGTGGGG CAAGTACTTG
1141GTGCAGTCGG AGCTTTACCT GAGATATTCA CAGAATTGGA AATTTCCTAT TTCCTGCTTA
1201GACGGCTTCT AGGTGTACGC ACTGAAGGAG ACAAGAAAGC AGCAAAGGTT CAAAAGCTGT
1261CTAAGAATGA AGTGCTCATG GTGAACATAG GATCCCTGTC AACAGGAGGG AGAGTTAGTG
1321CTGTCAAGGC CGATTTGGGT AAAATTGTTT TGACCAATCC AGTGTGCACA GAGGTAGGAG
1381AAAAAATTGC CCTTAGCCGA AGAGTTGAAA AACACTGGCG TTTAATTGGT TGGGGTCAGA
1441TAAGAAGAGG AGTGACAATC AACCAACAGT AGATGATGAC TGCCCAACTT TCTTGTACAA
1501AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA
1561ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA
1621GGACGATCAT TTAGTGTGGC CCTGCCGTAC ACGCGTTAAG TCgacaatca acctctggat
1681tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)