Construct: ORF TRCN0000467820
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002103.1_s317c1
- Derived from:
- ccsbBroadEn_08575
- DNA Barcode:
- CCCCACCAGAACCCCCGATCTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EDEM2 (55741)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467820
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55741 | EDEM2 | ER degradation enhancing al... | NM_018217.3 | 99.9% | 99.8% | 1366G>A |
| 2 | human | 55741 | EDEM2 | ER degradation enhancing al... | NM_001145025.2 | 93.5% | 93.4% | 104_105ins111;1255G>A |
| 3 | human | 111089941 | MMP24-AS1-EDEM2 | MMP24-AS1-EDEM2 readthrough | NM_001355008.2 | 92.8% | 92.7% | 0_1ins123;1243G>A |
| 4 | human | 55741 | EDEM2 | ER degradation enhancing al... | NR_026728.2 | 82.5% | (many diffs) | |
| 5 | mouse | 108687 | Edem2 | ER degradation enhancer, ma... | NM_145537.2 | 88.5% | 93.6% | (many diffs) |
| 6 | mouse | 108687 | Edem2 | ER degradation enhancer, ma... | XM_006498555.1 | 45.5% | 47.1% | (many diffs) |
| 7 | mouse | 108687 | Edem2 | ER degradation enhancer, ma... | XR_866205.2 | 42% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1800
- ORF length:
- 1734
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc tttccggctg ctcatcccgc tcggcctcct gtgcgcgctg ctgcctcagc 121 accatggtgc gccaggtccc gacggctccg cgccagatcc cgcccactac agggagcgag 181 tcaaggccat gttctaccac gcctacgaca gctacctgga gaatgccttt cccttcgatg 241 agctgcgacc tctcacctgt gacgggcacg acacctgggg cagtttttct ctgactctaa 301 ttgatgcact ggacaccttg ctgattttgg ggaatgtctc agaattccaa agagtggttg 361 aagtgctcca ggacagcgtg gactttgata ttgatgtgaa cgcctctgtg tttgaaacaa 421 acattcgagt ggtaggagga ctcctgtctg ctcatctgct ctccaagaag gctggggtgg 481 aagtagaggc tggatggccc tgttccgggc ctctcctgag aatggctgag gaggcggccc 541 gaaaactcct cccagccttt cagaccccca ctggcatgcc atatggaaca gtgaacttac 601 ttcatggcgt gaacccagga gagacccctg tcacctgtac ggcagggatt gggaccttca 661 ttgttgaatt tgccaccctg agcagcctca ctggtgaccc ggtgttcgaa gatgtggcca 721 gagtggcttt gatgcgcctc tgggagagcc ggtcagatat cgggctggtc ggcaaccaca 781 ttgatgtgct cactggcaag tgggtggccc aggacgcagg catcggggct ggcgtggact 841 cctactttga gtacttggtg aaaggagcca tcctgcttca ggataagaag ctcatggcca 901 tgttcctaga gtataacaaa gccatccgga actacacccg cttcgatgac tggtacctgt 961 gggttcagat gtacaagggg actgtgtcca tgccagtctt ccagtccttg gaggcctact 1021 ggcctggtct tcagagcctc attggagaca ttgacaatgc catgaggacc ttcctcaact 1081 actacactgt atggaagcag tttggggggc tcccggaatt ctacaacatt cctcagggat 1141 acacagtgga gaagcgagag ggctacccac ttcggccaga acttattgaa agcgcaatgt 1201 acctctaccg tgccacgggg gatcccaccc tcctagaact cggaagagat gctgtggaat 1261 ccattgaaaa aatcagcaag gtggagtgcg gatttgcaac aatcaaagat ctgcgagacc 1321 acaagctgga caaccgcatg gagtcgttct tcctggccga gactgtgaaa tacctctacc 1381 tcctgtttga cccaaccaac ttcatccaca acaatgggtc caccttcgac acggtgatca 1441 ccccctatgg ggagtgcatc ctgggggctg gggggtacat cttcaacaca gaagctcacc 1501 ccatcgacCC TGCCGCCCTG CACTGCTGCC AGAGGCTGAA GGAAGAGCAG TGGGAGGTGG 1561 AGGACTTGAT GAGGGAATTC TACTCTCTCA AACGGAGCAG GTCGAAATTT CAGAAAAACA 1621 CTGTTAGTTC GGGGCCATGG GAACCTCCAG CAAGGCCAGG AACACTCTTC TCACCAGAAA 1681 ACCATGACCA GGCAAGGGAG AGGAAGCCTG CCAAACAGAA GGTCCCACTT CTCAGCTGCC 1741 CCAGTCAGCC CTTCACCTCC AAGTTGGCAT TACTGGGACA GGTTTTCCTA GACTCCTCAT 1801 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1861 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1921 TTTATATATC TTGTGGAAAG GACGACCCCA CCAGAACCCC CGATCTGTCA CGCGTTAAGT 1981 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt