Construct: ORF TRCN0000467825
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005351.1_s317c1
- Derived from:
- ccsbBroadEn_06850
- DNA Barcode:
- ACCAGCCCAGTACCGGTAATTGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RBP2 (5948)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467825
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5948 | RBP2 | retinol binding protein 2 | NM_004164.3 | 99.5% | 100% | 6A>G;402A>G |
| 2 | mouse | 19660 | Rbp2 | retinol binding protein 2, ... | NM_009034.4 | 87.3% | 91% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 468
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac gagggaccag aatggaaccT GGGAGATGGA GAGTAATGAA AACTTTGAGG 121 GCTACATGAA GGCCCTGGAT ATTGATTTTG CCACCCGCAA GATTGCAGTA CGTCTCACTC 181 AGACGAAGGT TATTGATCAA GATGGTGATA ACTTCAAGAC AAAAACCACT AGCACATTCC 241 GCAACTATGA TGTGGATTTC ACTGTTGGAG TAGAGTTTGA CGAGTACACA AAGAGCCTGG 301 ATAACCGGCA TGTTAAGGCA CTGGTCACCT GGGAAGGTGA TGTCCTTGTG TGTGTGCAAA 361 AGGGGGAGAA GGAGAACCGC GGCTGGAAGC AGTGGATTGA GGGGGACAAG CTGTACCTGG 421 AGCTGACCTG TGGTGACCAG GTGTGCCGTC AAGTGTTCAA AAAGAAGTGC CCAACTTTCT 481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 601 GTGGAAAGGA CGAACCAGCC CAGTACCGGT AATTGCCACG CGTTAAGTCg acaatcaacc 661 tctggattac aaaatttgtg aaagatt