Construct: ORF TRCN0000467869

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010685.1_s317c1
Derived from:
ccsbBroadEn_00651
DNA Barcode:
TGTGAGGTTCTAACACACTTTTAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GNAI3 (2773)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467869
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2773 GNAI3 G protein subunit alpha i3 NM_006496.4 100% 100%
2 human 2770 GNAI1 G protein subunit alpha i1 NM_002069.6 80.9% 93.7% (many diffs)
3 human 2770 GNAI1 G protein subunit alpha i1 NM_001256414.2 68.3% 79.3% (many diffs)
4 mouse 14679 Gnai3 guanine nucleotide binding ... NM_010306.3 93.5% 98.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1128
ORF length:
1062
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggg ctgcacgttg agcgccgaag acaaggcggc agtggagcga agcaagatga
121tcgaccgcaa cttacgggag gacggggaaa aagcggccaa agaagtgaag ctgctgctac
181tcggtgctgg agaatctggt aaaagcacca ttgtgaaaca gatgaaaatc attcatgagg
241atggctattc agaggatgaa tgtaaacaat ataaagtagt tgtctacagc aatactatac
301agtccatcat tgcaatcata agagccatgg gacggctaaa gattgacttt ggggaagctg
361ccagggcaga tgatgcccgg caattatttg ttttagctgg cagtgctgaa gaaggagtca
421tgactccaga actagcagga gtgattaaac ggttatggcg agatggtggg gtacaagctt
481gcttcagcag atccagggaa tatcagctca atgattctgc ttcatattat ctaaatgatc
541tggatagaat atcccagtct aactacattc caactcagca agatgttctt cggacgagag
601tgaagaccac aggcattgta gaaacacatt tcaccttcaa agacctatac ttcaagatgt
661ttgatgtagg tggccaaaga tcagaacgaa aaaagtggat tcactgtttt gagggagtga
721cagcaattat cttctgtgtg gcccTCAGTG ATTATGACCT TGTTCTGGCT GAGGACGAGG
781AGATGAACCG AATGCATGAA AGCATGAAAC TGTTTGACAG CATTTGTAAT AACAAATGGT
841TTACAGAAAC TTCAATCATT CTCTTCCTTA ACAAGAAAGA CCTTTTTGAG GAAAAAATAA
901AGAGGAGTCC GTTAACTATC TGTTATCCAG AATACACAGG TTCCAATACA TATGAAGAGG
961CAGCTGCCTA TATTCAATGC CAGTTTGAAG ATCTGAACAG AAGAAAAGAT ACCAAGGAGA
1021TCTATACTCA CTTCACCTGT GCCACAGACA CGAAGAATGT GCAGTTTGTT TTTGATGCTG
1081TTACAGATGT CATCATTAAA AACAACTTAA AGGAATGTGG ACTTTATTGC CCAACTTTCT
1141TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA
1201CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT
1261GTGGAAAGGA CGATGTGAGG TTCTAACACA CTTTTATACG CGTTAAGTCg acaatcaacc
1321tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)