Construct: ORF TRCN0000467869
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010685.1_s317c1
- Derived from:
- ccsbBroadEn_00651
- DNA Barcode:
- TGTGAGGTTCTAACACACTTTTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GNAI3 (2773)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467869
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2773 | GNAI3 | G protein subunit alpha i3 | NM_006496.4 | 100% | 100% | |
| 2 | human | 2770 | GNAI1 | G protein subunit alpha i1 | NM_002069.6 | 80.9% | 93.7% | (many diffs) |
| 3 | human | 2770 | GNAI1 | G protein subunit alpha i1 | NM_001256414.2 | 68.3% | 79.3% | (many diffs) |
| 4 | mouse | 14679 | Gnai3 | guanine nucleotide binding ... | NM_010306.3 | 93.5% | 98.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1128
- ORF length:
- 1062
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgcacgttg agcgccgaag acaaggcggc agtggagcga agcaagatga 121 tcgaccgcaa cttacgggag gacggggaaa aagcggccaa agaagtgaag ctgctgctac 181 tcggtgctgg agaatctggt aaaagcacca ttgtgaaaca gatgaaaatc attcatgagg 241 atggctattc agaggatgaa tgtaaacaat ataaagtagt tgtctacagc aatactatac 301 agtccatcat tgcaatcata agagccatgg gacggctaaa gattgacttt ggggaagctg 361 ccagggcaga tgatgcccgg caattatttg ttttagctgg cagtgctgaa gaaggagtca 421 tgactccaga actagcagga gtgattaaac ggttatggcg agatggtggg gtacaagctt 481 gcttcagcag atccagggaa tatcagctca atgattctgc ttcatattat ctaaatgatc 541 tggatagaat atcccagtct aactacattc caactcagca agatgttctt cggacgagag 601 tgaagaccac aggcattgta gaaacacatt tcaccttcaa agacctatac ttcaagatgt 661 ttgatgtagg tggccaaaga tcagaacgaa aaaagtggat tcactgtttt gagggagtga 721 cagcaattat cttctgtgtg gcccTCAGTG ATTATGACCT TGTTCTGGCT GAGGACGAGG 781 AGATGAACCG AATGCATGAA AGCATGAAAC TGTTTGACAG CATTTGTAAT AACAAATGGT 841 TTACAGAAAC TTCAATCATT CTCTTCCTTA ACAAGAAAGA CCTTTTTGAG GAAAAAATAA 901 AGAGGAGTCC GTTAACTATC TGTTATCCAG AATACACAGG TTCCAATACA TATGAAGAGG 961 CAGCTGCCTA TATTCAATGC CAGTTTGAAG ATCTGAACAG AAGAAAAGAT ACCAAGGAGA 1021 TCTATACTCA CTTCACCTGT GCCACAGACA CGAAGAATGT GCAGTTTGTT TTTGATGCTG 1081 TTACAGATGT CATCATTAAA AACAACTTAA AGGAATGTGG ACTTTATTGC CCAACTTTCT 1141 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 1201 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 1261 GTGGAAAGGA CGATGTGAGG TTCTAACACA CTTTTATACG CGTTAAGTCg acaatcaacc 1321 tctggattac aaaatttgtg aaagatt