Construct: ORF TRCN0000467876
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003582.1_s317c1
- Derived from:
- ccsbBroadEn_07045
- DNA Barcode:
- AGTCAGAACTTTTGAAATTTTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TEAD4 (7004)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467876
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7004 | TEAD4 | TEA domain transcription fa... | NM_201443.3 | 84.4% | 84.4% | 0_1ins168 |
| 2 | human | 7004 | TEAD4 | TEA domain transcription fa... | NM_003213.4 | 83.1% | 83.1% | 1_219del |
| 3 | human | 7004 | TEAD4 | TEA domain transcription fa... | NM_201441.3 | 73.2% | 73.2% | 1_219del;353_354ins129 |
| 4 | mouse | 21679 | Tead4 | TEA domain family member 4 | NM_011567.2 | 75.2% | 80% | (many diffs) |
| 5 | mouse | 21679 | Tead4 | TEA domain family member 4 | NM_001080979.1 | 66.1% | 71.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1149
- ORF length:
- 1083
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta tggtcggaac gagctgattg cccgctacat caagctccgg acagggaaga 121 cccgcaccag gaagcaggtc tccagccaca tccaggtgct ggctcgtcgc aaagctcgcg 181 agatccaggc caagctaaag gaccaggcag ctaaggacaa ggccctgcag agcatggctg 241 ccatgtcgtc tgcacagatc atctccgcca cggccttcca cagtagcatg gccctcgccc 301 ggggccccgg ccgcccagca gtctcagggt tttggcaagg agctttgcca ggccaagccg 361 gaacgtccca tgatgtgaag cctttctctc agcaaaccta tgctgtccag cctccgctgc 421 ctctgccagg gtttgagtct cctgcagggc ccgccccatc gccctctgcg cccccggcac 481 ccccatggca gggccgcagc gtggccagct ccaagctctg gatgttggag ttctctgcct 541 tcctggagca gcagcaggac ccggacacgt acaacaagca cctgttcgtg cacattggcc 601 agtccagccc aagctacagc gacccctacc tcgaagccgt ggacatccgc caaatctatg 661 acaaattccc ggagaaaaag ggtggactca aggatctctt cgaacgggga ccctccaatg 721 ccttttttct tgtgaagttc tgggcagacc tcaacaccaa catcgaggat gaaggcagct 781 ccttctatgg ggtctccagc cagtatgaga gccccgagaa catgatcatc acctgcTCCA 841 CGAAGGTCTG CTCTTTCGGC AAGCAGGTGG TGGAGAAAGT TGAGACAGAG TATGCTCGCT 901 ATGAGAATGG ACACTACTCT TACCGCATCC ACCGGTCCCC GCTCTGTGAG TACATGATCA 961 ACTTCATCCA CAAGCTCAAG CACCTCCCTG AGAAGTACAT GATGAACAGC GTGCTGGAGA 1021 ACTTCACCAT CCTGCAGGTG GTCACCAACA GAGACACACA GGAGACCTTG CTGTGCATTG 1081 CCTATGTCTT TGAGGTGTCA GCCAGTGAGC ACGGGGCTCA GCACCACATC TACAGGCTGG 1141 TGAAAGAATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1201 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1261 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAGTCAG AACTTTTGAA ATTTTATTAC 1321 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt