Construct: ORF TRCN0000467878
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017761.1_s317c1
- Derived from:
- ccsbBroadEn_11892
- DNA Barcode:
- CTGCCTACTGGTCGGCAGCCTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MACROD1 (28992)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467878
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 28992 | MACROD1 | mono-ADP ribosylhydrolase 1 | NM_014067.4 | 74.7% | 74.7% | 1_246del |
| 2 | human | 28992 | MACROD1 | mono-ADP ribosylhydrolase 1 | XM_005273939.4 | 72.6% | 72.4% | 1_246del;974A>C;976_1002del |
| 3 | human | 28992 | MACROD1 | mono-ADP ribosylhydrolase 1 | XM_011544970.2 | 69.6% | 69.4% | (many diffs) |
| 4 | human | 28992 | MACROD1 | mono-ADP ribosylhydrolase 1 | XM_006718521.3 | 32.4% | 30.8% | (many diffs) |
| 5 | human | 28992 | MACROD1 | mono-ADP ribosylhydrolase 1 | XM_005273940.2 | 30.5% | 28.6% | (many diffs) |
| 6 | human | 28992 | MACROD1 | mono-ADP ribosylhydrolase 1 | XM_006718522.2 | 27.9% | 27.6% | 1_246del;517_519delGGA;522_523ins456 |
| 7 | mouse | 107227 | Macrod1 | MACRO domain containing 1 | NM_134147.4 | 65.6% | 68.7% | (many diffs) |
| 8 | mouse | 107227 | Macrod1 | MACRO domain containing 1 | XM_006526562.2 | 63.6% | 66.1% | (many diffs) |
| 9 | mouse | 107227 | Macrod1 | MACRO domain containing 1 | XM_006526563.2 | 60.9% | 63.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 795
- ORF length:
- 729
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgaaggtg gacctgagca cctccaccga ctggaaggag gcgaaatcct 121 ttctgaaggg cctgagtgac aagcagcggg aggaacatta cttctgcaag gactttgtca 181 ggctgaagaa gatcccgaca tggaaggaga tggcgaaagg ggtggctgtg aaggtggagg 241 agcccaggta taaaaaggac aagcagctca atgagaaaat ctccctgctc cgcagcgaca 301 tcaccaagct ggaggtggac gccatcgtca acgccgccaa cagctccctg cTCGGAGGCG 361 GTGGCGTGGA CGGCTGCATT CATCGGGCCG CCGGCCCCCT GCTTACCGAC GAGTGCCGGA 421 CCCTGCAGAG CTGTAAGACT GGCAAGGCCA AGATCACCGG CGGCTATCGG CTCCCGGCCA 481 AGTACGTCAT CCACACAGTG GGGCCCATCG CCTACGGGGA GCCCAGCGCC AGTCAGGCTG 541 CCGAGCTCCG CAGCTGCTAC CTGAGCAGTC TGGACCTGCT GCTGGAGCAC CGGCTCCGCT 601 CGGTGGCGTT CCCCTGCATC TCCACCGGCG TGTTTGGCTA CCCCTGTGAG GCGGCCGCCG 661 AGATCGTGCT GGCCACGCTG CGAGAGTGGC TGGAGCAGCA CAAGGACAAG GTGGACCGGC 721 TGATCATCTG CGTGTTCCTC GAGAAGGACG AGGACATCTA CCGGAGCCGG CTCCCCCACT 781 ACTTCCCCGT GGCCTGCCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 841 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 901 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA CTGCCTACTG GTCGGCAGCC 961 TAACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt