Construct: ORF TRCN0000467927
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004115.1_s317c1
- Derived from:
- ccsbBroadEn_08896
- DNA Barcode:
- CCTCCCATATTCTGTTCTGTTGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP4F12 (66002)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467927
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | NM_023944.4 | 99.8% | 99.4% | 47C>T;226A>G;562C>T |
| 2 | human | 57834 | CYP4F11 | cytochrome P450 family 4 su... | NM_001128932.1 | 86.8% | 82.8% | (many diffs) |
| 3 | human | 57834 | CYP4F11 | cytochrome P450 family 4 su... | NM_021187.3 | 86.8% | 82.8% | (many diffs) |
| 4 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | NM_001199208.2 | 86% | 80.7% | (many diffs) |
| 5 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | NM_001199209.2 | 86% | 80.7% | (many diffs) |
| 6 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | NM_001369696.1 | 86% | 80.7% | (many diffs) |
| 7 | human | 8529 | CYP4F2 | cytochrome P450 family 4 su... | NM_001082.5 | 85.9% | 81.2% | (many diffs) |
| 8 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | NM_000896.3 | 81.9% | 77.6% | (many diffs) |
| 9 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_017027172.1 | 78.2% | 70% | (many diffs) |
| 10 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | NR_117085.2 | 74.2% | (many diffs) | |
| 11 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_006722850.3 | 71.5% | 71.3% | 0_1ins447;115C>T |
| 12 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_011528202.3 | 71.5% | 71.3% | 0_1ins447;115C>T |
| 13 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_011528208.2 | 71.5% | 71.3% | 0_1ins447;115C>T |
| 14 | human | 11283 | CYP4F8 | cytochrome P450 family 4 su... | XM_024451341.1 | 67.9% | 62.5% | (many diffs) |
| 15 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_011528203.2 | 66.6% | 66.4% | (many diffs) |
| 16 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_011528204.2 | 66.6% | 66.4% | (many diffs) |
| 17 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_011528205.2 | 66.6% | 66.4% | (many diffs) |
| 18 | human | 66002 | CYP4F12 | cytochrome P450 family 4 su... | XM_011528207.2 | 63.7% | 63.7% | 0_1ins570 |
| 19 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | XM_017026815.1 | 63.2% | 57.2% | (many diffs) |
| 20 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | XM_005259911.5 | 62.4% | 60.3% | (many diffs) |
| 21 | human | 4051 | CYP4F3 | cytochrome P450 family 4 su... | XM_011528014.3 | 62.4% | 60.3% | (many diffs) |
| 22 | human | 388514 | CYP4F24P | cytochrome P450 family 4 su... | NR_033864.1 | 40% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1638
- ORF length:
- 1572
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gctgctgagc ctgccctggc tgggcctcag accggtggca atgtccccat 121 ggctactcct gctgctggtt gtgggctcct ggctactcgc ccgcatcctg gcttggacct 181 atgccttcta taacaactgc cgccggctcc agtgtttccc acagccccca aaacggaact 241 ggttttgggg tcacctgggc ctgatcactc ctacagagga gggcttgaag gactcgaccc 301 agatgtcggc cacctattcc cagggcttta cggtatggct gggtcccatc atccccttca 361 tcgttttatg ccaccctgac accatccggt ctatcaccaa tgcctcagct gccattgcac 421 ccaaggataa tctcttcatc aggttcctga agccctggct gggagaaggg atactgctga 481 gtggcggtga caagtggagc cgccaccgtc ggatgctgac gcccgccttc catttcaaca 541 tcctgaagtc ctatataacg atcttcaaca agagtgcaaa catcatgctt gacaagtggc 601 agcacctggc ctcagagggc agcagttgtc tggacatgtt tgagcacatc agcctcatga 661 ccttggacag tctacagaaa tgcatcttca gctttgacag ccattgtcag gagaggccca 721 gtgaatatat tgccaccatc ttggagctca gtgcccttgt agagaaaaga agccagcata 781 tcctccagca catggacttt ctgtattacc tctcccatga cgggcggcgc ttccacaggg 841 cctgccgcct ggtgcatgac ttcacagacg ctgtcatccg ggagcggcgt cgcaccctcc 901 ccactcaggg tattgatgat tttttcaaag acaaagccaa gtccaagact ttggatttca 961 ttgatgtgct tctgctgagc aaggatgaag atgggaaggc attgtcagat gaggatataa 1021 gagcagaggc tgacaccttc atgtttggag gccatgacac cacggccagt ggcctctcct 1081 gggtcctgta caaccttgcg aggcacccag aataccagga gcgctgccga caggaggtgc 1141 aagagcttct gaaggaccgc gatcctaaag agattgaatg ggacgacctg gcccagctgc 1201 ccTTCCTGAC CATGTGCGTG AAGGAGAGCC TGAGGTTACA TCCCCCAGCT CCCTTCATCT 1261 CCCGATGCTG CACCCAGGAC ATTGTTCTCC CAGATGGCCG AGTCATCCCC AAAGGCATTA 1321 CCTGCCTCAT CGATATTATA GGGGTCCATC ACAACCCAAC TGTGTGGCCG GATCCTGAGG 1381 TCTACGACCC CTTCCGCTTT GACCCAGAGA ACAGCAAGGG GAGGTCACCT CTGGCTTTTA 1441 TTCCTTTCTC CGCAGGGCCC AGGAACTGCA TCGGGCAGGC GTTCGCCATG GCGGAGATGA 1501 AAGTGGTCCT GGCGTTGATG CTGCTGCACT TCCGGTTCCT GCCAGACCAC ACTGAGCCCC 1561 GCAGGAAGCT GGAATTGATC ATGCGCGCCG AGGGCGGGCT TTGGCTGCGG GTGGAGCCCC 1621 TGAATGTAAG CTTGCAGAGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1681 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1741 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCTCCCA TATTCTGTTC 1801 TGTTGACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt