Construct: ORF TRCN0000467930
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003465.1_s317c1
- Derived from:
- ccsbBroadEn_14234
- DNA Barcode:
- TTTCGTATACAAAATACCATCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RNF213 (57674)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467930
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57674 | RNF213 | ring finger protein 213 | XM_017024905.2 | 10.3% | 10.3% | 1_13236del;14759T>C |
| 2 | human | 57674 | RNF213 | ring finger protein 213 | NM_001256071.3 | 9.7% | 9.7% | 1_14094del;15617T>C |
| 3 | human | 57674 | RNF213 | ring finger protein 213 | XM_005257545.4 | 9.6% | 9.6% | 1_14241del;15764T>C |
| 4 | human | 57674 | RNF213 | ring finger protein 213 | XM_005257546.4 | 9.6% | 9.6% | 1_14241del;15764T>C |
| 5 | human | 57674 | RNF213 | ring finger protein 213 | XM_006721995.3 | 9.6% | 9.6% | 1_14241del;15764T>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1593
- ORF length:
- 1527
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa taatctcatc agccaagata agcgtatcag ctctaaccct gtggccaaaa 121 taatatatgg tgacccagtg accttcctgc cccacctgcc ccggaaaagt gtggtccatt 181 gctctaagat ttggagctgc aggaaaagaa ttacagttga gtacctccag cacattgtgg 241 aacagaaaaa tggcaaagaa agagtgccca tcctctggca tttcctgcag aaggaagcag 301 agctgaggct ggtaaagttc ctgcctgaga ttttggcctt gcaaagggat ctagtgaagc 361 agttccagaa cgtccagcaa gttgaataca gctccatcag aggcttcctc agcaagcaca 421 gctcagatgg gttgaggcag ctgcttcaca acaggatcac agtctttctg tccacatgga 481 acaaactgag gagatcgctt gagacgaacg gtgagatcaa cctacccaaa gactactgca 541 gcactgactt ggatctggac actgagtttg agatcctctt gccacgccga cggggcctgg 601 gcctctgtgc taccgctctc gtcagctact tgattcgcct acacaatgaa attgtctacg 661 ccgtggaaaa actctccaag gaaaacaaca gctattccgt ggatgccgcc gaggtcactg 721 aactgcatgt catcagttat gaagtggagc gggacctgac tccactgatt ctctccaact 781 gccagtacca ggtggaggag ggcagagaga ccgtgcagga gttcgatctg gagaagattc 841 agcggcagat cgtcagccgc ttcctccagg gcaagccccg gctgagcctc aagggaatac 901 ccactctggt gtacagacac gactggaact atgaacatct ctttatggac atcaagaaca 961 aaatggcaca ggactccctc cccagctcgg tcattagtgc catcagtgga cagctgcagt 1021 cctacagcga tgcctgtgaa gtgctgtctg tcgtagaagt cactctgggg tttctgagca 1081 cagctggtgg ggatccaaac atgcagctga atgtgtatac tcaagacatc ctgcaaatgg 1141 gtgatcagac gattcacgtg ttaaaggCCT TAAACAGATG CCAGTTAAAA CACACCATTG 1201 CCCTCTGGCA GTTCCTGTCT GCTCATAAGT CTGAACAGCT GCTGCGGCTG CACAAAGAGC 1261 CATTTGGGGA AATCAGTTCA AGGTACAAAG CGGATCTGAG CCCGGAAAAT GCTAAGCTCC 1321 TCAGCACATT CCTAAATCAG ACTGGCCTAG ACGCCTTCCT GCTAGAGCTG CACGAAATGA 1381 TAATCTTGAA ACTAAAGAAC CCCCAAACCC AAACCGAGGA GCGCTTCCGC CCTCAGTGGA 1441 GCCTGAGAGA CACTCTCGTA AGTTACATGC AAACTAAAGA AAGTGAAATT CTTCCTGAAA 1501 TGGCATCTCA GTTCCCAGAA GAGATACTGC TCGCCAGCTG TGTCTCAGTG TGGAAAACAG 1561 CTGCTGTGCT GAAATGGAAT CGAGAAACGA GATACCCAAC TTTCTTGTAC AAAGTGGTTG 1621 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1681 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATT 1741 TCGTATACAA AATACCATCA TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 1801 ttgtgaaaga tt