Construct: ORF TRCN0000467933
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004361.1_s317c1
- Derived from:
- ccsbBroadEn_14156
- DNA Barcode:
- CGGATACTACGACGTCTTTCTTTA
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHAX (51808)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467933
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51808 | PHAX | phosphorylated adaptor for ... | NM_032177.4 | 99.9% | 98.7% | 1167delT |
| 2 | mouse | 56698 | Phax | phosphorylated adaptor for ... | NM_019996.4 | 81.6% | 84.8% | (many diffs) |
| 3 | mouse | 56698 | Phax | phosphorylated adaptor for ... | NM_001162989.1 | 76.3% | 78.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1248
- ORF length:
- 1182
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gttggaggtc ggcgatatgg aagatgggca gctttccgac tcggattccg 121 acatgacggt cgcacccagc gacaggccgc tgcaattgcc aaaagtgcta ggtggcgaca 181 gtgctatgag ggccttccag aacacggcaa ctgcatgtgc accagtatca cattatcgag 241 ctgttgaaag tgtggattca agtgaagaaa gtttttctga ttcagatgat gatagctgtc 301 tttggaaacg caaacgacag aaatgtttta accctcctcc caaaccagag ccttttcagt 361 ttggccagag cagtcagaaa ccacctgttg ctggaggaaa gaagattaac aacatatggg 421 gtgctgtgct gcaggaacag aatcaagatg cagtggccac tgaacttggt atcttgggaa 481 tggagggcac tattgacaga agcagacaat ccgagaccta caattatttg cttgccaaga 541 aacttaggaa ggaatctcaa gagcatacaa aagatctaga caaggaacta gatgaatata 601 tgcatggtgg caaaaaaatg ggatcaaagg aagaggaaaa tgggcaaggt catctcaaaa 661 ggaaacgacc tgtcaaagac aggctaggga acagaccaga aatgaactat aaaggtcgat 721 acgagatcac agcggaagat tctcaagaga aagtggctga tgaaatttca ttcaggttac 781 aggaaccaaa gaaagacctg atagcccgag tagtgaggat tattggtaac aaaaaggcaa 841 ttgaacttcT GATGGAAACC GCTGAAGTTG AACAAAATGG TGGTCTCTTT ATAATGAATG 901 GTAGTCGAAG AAGAACACCA GGTGGAGTTT TTCTGAATCT CTTGAAAAAC ACTCCTAGTA 961 TCAGCGAGGA ACAAATTAAG GACATTTTCT ACATTGAAAA CCAAAAGGAA TATGAAAATA 1021 AAAAAGCTGC TAGGAAGAGG AGAACACAAG TGTTGGGGAA AAAGATGAAA CAAGCTATTA 1081 AAAGTCTAAA TTTTCAAGAA GATGATGATA CATCACGAGA AACTTTTGCA AGTGACACGA 1141 ATGAGGCCTT GGCCTCTCTT GATGAGTCAC AGGAAGGACA TGCAGAAGCC AAGTTGGAGG 1201 CAGAGGAAGC CATTGAAGTT GATCATTCTC AGATTTGGAC ATCTTTTACC CAACTTTCTT 1261 GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC 1321 GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG 1381 TGGAAAGGAC GACGGATACT ACGACGTCTT TCTTTAACGC GTTAAGTCga caatcaacct 1441 ctggattaca aaatttgtga aagatt