Construct: ORF TRCN0000467933

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004361.1_s317c1
Derived from:
ccsbBroadEn_14156
DNA Barcode:
CGGATACTACGACGTCTTTCTTTA
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PHAX (51808)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467933
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51808 PHAX phosphorylated adaptor for ... NM_032177.4 99.9% 98.7% 1167delT
2 mouse 56698 Phax phosphorylated adaptor for ... NM_019996.4 81.6% 84.8% (many diffs)
3 mouse 56698 Phax phosphorylated adaptor for ... NM_001162989.1 76.3% 78.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1248
ORF length:
1182
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc gttggaggtc ggcgatatgg aagatgggca gctttccgac tcggattccg
121acatgacggt cgcacccagc gacaggccgc tgcaattgcc aaaagtgcta ggtggcgaca
181gtgctatgag ggccttccag aacacggcaa ctgcatgtgc accagtatca cattatcgag
241ctgttgaaag tgtggattca agtgaagaaa gtttttctga ttcagatgat gatagctgtc
301tttggaaacg caaacgacag aaatgtttta accctcctcc caaaccagag ccttttcagt
361ttggccagag cagtcagaaa ccacctgttg ctggaggaaa gaagattaac aacatatggg
421gtgctgtgct gcaggaacag aatcaagatg cagtggccac tgaacttggt atcttgggaa
481tggagggcac tattgacaga agcagacaat ccgagaccta caattatttg cttgccaaga
541aacttaggaa ggaatctcaa gagcatacaa aagatctaga caaggaacta gatgaatata
601tgcatggtgg caaaaaaatg ggatcaaagg aagaggaaaa tgggcaaggt catctcaaaa
661ggaaacgacc tgtcaaagac aggctaggga acagaccaga aatgaactat aaaggtcgat
721acgagatcac agcggaagat tctcaagaga aagtggctga tgaaatttca ttcaggttac
781aggaaccaaa gaaagacctg atagcccgag tagtgaggat tattggtaac aaaaaggcaa
841ttgaacttcT GATGGAAACC GCTGAAGTTG AACAAAATGG TGGTCTCTTT ATAATGAATG
901GTAGTCGAAG AAGAACACCA GGTGGAGTTT TTCTGAATCT CTTGAAAAAC ACTCCTAGTA
961TCAGCGAGGA ACAAATTAAG GACATTTTCT ACATTGAAAA CCAAAAGGAA TATGAAAATA
1021AAAAAGCTGC TAGGAAGAGG AGAACACAAG TGTTGGGGAA AAAGATGAAA CAAGCTATTA
1081AAAGTCTAAA TTTTCAAGAA GATGATGATA CATCACGAGA AACTTTTGCA AGTGACACGA
1141ATGAGGCCTT GGCCTCTCTT GATGAGTCAC AGGAAGGACA TGCAGAAGCC AAGTTGGAGG
1201CAGAGGAAGC CATTGAAGTT GATCATTCTC AGATTTGGAC ATCTTTTACC CAACTTTCTT
1261GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC
1321GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG
1381TGGAAAGGAC GACGGATACT ACGACGTCTT TCTTTAACGC GTTAAGTCga caatcaacct
1441ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)