Construct: ORF TRCN0000467935
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017985.1_s317c1
- Derived from:
- ccsbBroadEn_13982
- DNA Barcode:
- GACGACACCCCTTCCACCAATGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- XBP1 (7494)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467935
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7494 | XBP1 | X-box binding protein 1 | NM_005080.3 | 99.8% | 99.6% | 10G>A |
| 2 | human | 7494 | XBP1 | X-box binding protein 1 | NM_001079539.1 | 65.5% | 41.5% | 10G>A;492_493ins26;758_1128del |
| 3 | mouse | 22433 | Xbp1 | X-box binding protein 1 | NM_013842.3 | 80.6% | 76.6% | (many diffs) |
| 4 | mouse | 22433 | Xbp1 | X-box binding protein 1 | NM_001271730.1 | 55.1% | 39% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 849
- ORF length:
- 783
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggtgatggca gccgcgccga acccggccga cgggacccct aaagttctgc 121 ttctgtcggg gcagcccgcc tccgccgccg gagccccggc cggccaggcc ctgccgctca 181 tggtgccagc ccagagaggg gccagcccgg aggcagcgag cggggggctg ccccaggcgc 241 gcaagcgaca gcgcctcacg cacctgagcc ccgaggagaa ggcgctgagg aggaaactga 301 aaaacagagt agcagctcag actgccagag atcgaaagaa ggctcgaatg agtgagctgg 361 aacagcaagt ggtagattta gaagaagaga accaaaaact tttgctagaa aatcagcttt 421 tacgagagaa aactcatggc cttGTAGTTG AGAACCAGGA GTTAAGACAG CGCTTGGGGA 481 TGGATGCCCT GGTTGCTGAA GAGGAGGCGG AAGCCAAGGG GAATGAAGTG AGGCCAGTGG 541 CCGGGTCTGC TGAGTCCGCA GCACTCAGAC TACGTGCACC TCTGCAGCAG GTGCAGGCCC 601 AGTTGTCACC CCTCCAGAAC ATCTCCCCAT GGATTCTGGC GGTATTGACT CTTCAGATTC 661 AGAGTCTGAT ATCCTGTTGG GCATTCTGGA CAACTTGGAC CCAGTCATGT TCTTCAAATG 721 CCCTTCCCCA GAGCCTGCCA GCCTGGAGGA GCTCCCAGAG GTCTACCCAG AAGGACCCAG 781 TTCCTTACCA GCCTCCCTTT CTCTGTCAGT GGGGACGTCA TCAGCCAAGC TGGAAGCCAT 841 TAATGAACTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 901 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 961 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAGACGAC ACCCCTTCCA CCAATGGTAC 1021 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt