Construct: ORF TRCN0000467943
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018800.2_s317c1
- Derived from:
- ccsbBroadEn_14967
- DNA Barcode:
- GTCCACGAAGTGATTACTTGTGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CIB1 (10519)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467943
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10519 | CIB1 | calcium and integrin binding 1 | NM_006384.4 | 100% | 100% | |
| 2 | human | 10519 | CIB1 | calcium and integrin binding 1 | NM_001277764.1 | 82.6% | 82.6% | 87_206del |
| 3 | human | 10519 | CIB1 | calcium and integrin binding 1 | XM_006720375.2 | 71.8% | 70.4% | (many diffs) |
| 4 | human | 10519 | CIB1 | calcium and integrin binding 1 | NR_102428.1 | 63% | (many diffs) | |
| 5 | human | 10519 | CIB1 | calcium and integrin binding 1 | NR_102427.1 | 55.2% | (many diffs) | |
| 6 | mouse | 23991 | Cib1 | calcium and integrin bindin... | NM_011870.5 | 88.9% | 93.7% | (many diffs) |
| 7 | mouse | 23991 | Cib1 | calcium and integrin bindin... | NM_001291275.1 | 86.6% | 91% | (many diffs) |
| 8 | mouse | 23991 | Cib1 | calcium and integrin bindin... | NM_001291276.1 | 67.7% | 72.2% | (many diffs) |
| 9 | mouse | 23991 | Cib1 | calcium and integrin bindin... | XM_011250861.2 | 54.9% | 58.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 642
- ORF length:
- 573
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggggctcg ggcagtcgcc tgtccaagga gctgctggcc gagtaccagg 121 acttgacgtt cctgacgaag caggagatcc tcctagccca caggcggttt tgtgagctgc 181 ttccccagga gcagcggagc gtggagtcgt cacttcgggc acaagtgccc ttcgagcaga 241 ttctcagcct tccagagctc aaggccaacc ccttcaagga gcgaatctgc agggTCTTCT 301 CCACATCCCC AGCCAAAGAC AGCCTTAGCT TTGAGGACTT CCTGGATCTC CTCAGTGTGT 361 TCAGTGACAC AGCCACGCCA GACATCAAGT CCCATTATGC CTTCCGCATC TTTGACTTTG 421 ATGATGACGG AACCTTGAAC AGAGAAGACC TGAGCCGGCT GGTGAACTGC CTCACGGGAG 481 AGGGCGAGGA CACACGGCTT AGTGCGTCTG AGATGAAGCA GCTCATCGAC AACATCCTGG 541 AGGAGTCTGA CATTGACAGG GATGGAACCA TCAACCTCTC TGAGTTCCAG CACGTCATCT 601 CCCGTTCTCC AGACTTTGCC AGCTCCTTTA AGATTGTCCT GTTGCCAACT TTCTTGTACA 661 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 721 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 781 AGGACGAGTC CACGAAGTGA TTACTTGTGC AACGCGTTAA GTCgacaatc aacctctgga 841 ttacaaaatt tgtgaaagat t