Construct: ORF TRCN0000467949
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000977.1_s317c1
- Derived from:
- ccsbBroadEn_07671
- DNA Barcode:
- TCAACACTCCCATCGGCATACTCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SIX2 (10736)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467949
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10736 | SIX2 | SIX homeobox 2 | NM_016932.5 | 99.8% | 99.6% | 472G>A |
| 2 | human | 10736 | SIX2 | SIX homeobox 2 | XM_005264100.3 | 99.2% | 98.9% | 472G>A;561_566delGTACGA |
| 3 | human | 6495 | SIX1 | SIX homeobox 1 | XM_017021602.2 | 52.6% | 55.3% | (many diffs) |
| 4 | mouse | 20472 | Six2 | sine oculis-related homeobox 2 | NM_011380.2 | 91.6% | 96.9% | (many diffs) |
| 5 | mouse | 20472 | Six2 | sine oculis-related homeobox 2 | XM_006523931.3 | 91% | 96.3% | (many diffs) |
| 6 | mouse | 20472 | Six2 | sine oculis-related homeobox 2 | XM_011246335.2 | 64.6% | 65% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 939
- ORF length:
- 873
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc catgctgccc accttcggct tcacgcagga gcaagtggcg tgcgtgtgcg 121 aggtgctgca gcagggcggc aacatcgagc ggctgggccg cttcctgtgg tcgctgcccg 181 cctgcgagca ccttcacaag aatgaaagcg tgctcaaggc caaggccgtg gtggccttcc 241 accgcggcaa cttccgcgag ctctacaaga tcctggagag ccaccagttc tcgccgcaca 301 accacgccaa gctgcagcag ctgtggctca aggcacacta catcgaggcg gagaagctgc 361 gcggccgacc cctgggcgcc gtgggcaaat accgcgtgcg ccgcaaattc ccgctgccgc 421 gctccatctg ggacggcgag gagaccagct actgcttcaa ggaaaagagt cgcagcgtgc 481 tgcgcgagtg gtacgcgcac aacccctacc cttcaccccg cgagaagcgt gagctgacgg 541 aggccacggg cctcaccaCC ACACAGGTCA GCAACTGGTT CAAGAACCGG CGGCAGCGCG 601 ACCGGGCGGC CGAGGCCAAG GAAAGGGAGA ACAACGAGAA CTCCAATTCT AACAGCCACA 661 ACCCGCTGAA TGGCAGCGGC AAGTCGGTGT TAGGCAGCTC GGAGGATGAG AAGACTCCAT 721 CGGGGACGCC AGACCACTCA TCATCCAGCC CCGCACTGCT CCTCAGCCCG CCGCCCCCTG 781 GGCTGCCGTC CCTGCACAGC CTGGGCCACC CTCCGGGCCC CAGCGCAGTG CCAGTGCCGG 841 TGCCAGGCGG AGGTGGAGCG GACCCACTGC AACACCACCA TGGCCTGCAG GACTCCATCC 901 TCAACCCCAT GTCAGCCAAC CTCGTGGACC TGGGCTCCTA CCCAACTTTC TTGTACAAAG 961 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1021 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1081 ACGATCAACA CTCCCATCGG CATACTCTAC GCGTTAAGTC gacaatcaac ctctggatta 1141 caaaatttgt gaaagatt