Construct: ORF TRCN0000467975
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013122.1_s317c1
- Derived from:
- ccsbBroadEn_09028
- DNA Barcode:
- AAATTTTCATGCAATATTGAGCCA
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- CEP70 (80321)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467975
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80321 | CEP70 | centrosomal protein 70 | NM_001320598.2 | 99.8% | 98.6% | 404G>A;462A>G;1771A>T |
| 2 | human | 80321 | CEP70 | centrosomal protein 70 | NM_024491.4 | 99.8% | 98.6% | 404G>A;462A>G;1771A>T |
| 3 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007275.1 | 99.8% | 98.6% | 404G>A;462A>G;1771A>T |
| 4 | human | 80321 | CEP70 | centrosomal protein 70 | NM_001288964.2 | 96.8% | 95.6% | (many diffs) |
| 5 | human | 80321 | CEP70 | centrosomal protein 70 | NM_001288965.2 | 96.4% | 95.3% | (many diffs) |
| 6 | human | 80321 | CEP70 | centrosomal protein 70 | NM_001320599.2 | 96.2% | 95.6% | (many diffs) |
| 7 | human | 80321 | CEP70 | centrosomal protein 70 | XM_024453781.1 | 96.2% | 95.6% | (many diffs) |
| 8 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007271.1 | 93.3% | 91.6% | (many diffs) |
| 9 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007272.1 | 93.3% | 91.6% | (many diffs) |
| 10 | human | 80321 | CEP70 | centrosomal protein 70 | XM_024453780.1 | 93.3% | 91.6% | (many diffs) |
| 11 | human | 80321 | CEP70 | centrosomal protein 70 | NM_001288966.1 | 92.6% | 93% | (many diffs) |
| 12 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007273.1 | 90.1% | 88.4% | (many diffs) |
| 13 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007274.1 | 90.1% | 88.4% | (many diffs) |
| 14 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007276.2 | 74.6% | 72.9% | (many diffs) |
| 15 | human | 80321 | CEP70 | centrosomal protein 70 | NM_001288967.2 | 74.3% | 72.8% | (many diffs) |
| 16 | human | 80321 | CEP70 | centrosomal protein 70 | XM_011513209.3 | 69.6% | 68.6% | (many diffs) |
| 17 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007277.1 | 69.2% | 67.2% | (many diffs) |
| 18 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007278.2 | 65.6% | 63.9% | (many diffs) |
| 19 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007279.1 | 57.6% | 55.3% | (many diffs) |
| 20 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007282.2 | 56.5% | 55.4% | 0_1ins777;994A>T |
| 21 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007283.1 | 56.5% | 55.4% | 0_1ins777;994A>T |
| 22 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007284.1 | 56.5% | 55.4% | 0_1ins777;994A>T |
| 23 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007280.2 | 52.5% | 50.7% | (many diffs) |
| 24 | human | 80321 | CEP70 | centrosomal protein 70 | XM_017007281.2 | 52.5% | 50.7% | (many diffs) |
| 25 | human | 80321 | CEP70 | centrosomal protein 70 | NM_001320600.1 | 35.3% | 35.7% | 404G>A;462A>G;636_637ins1155 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1836
- ORF length:
- 1770
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt tccggtagcc cctaaacccc aggattccag tcaaccatca gacagactca 121 tgactgaaaa acagcaggaa gaagcagaat gggaaagcat aaatgtgcta ttgatgatgc 181 atggcttaaa acctttgtct ctagtcaaaa gaacagatct caaagatctc atcatttttg 241 acaaacagtc atcacaaagg atgagacaga atttgaaatt gttggtggaa gaaacatcat 301 gtcaacagaa catgatacag gagcttatag aaactaatca acagcttaga aatgaacttc 361 agctagagca aagccgagca gccaatcaag aacaacgagc taatgacttg gaacaaatta 421 tggaaagtgt gaaatccaaa attggtgaat tggaggatga atcactaaat agggcttgcc 481 accaacagaa taaaataaaa gatcttcaaa aggagcagaa aactttgcag gtgaagtgcc 541 agcattataa gaaaaaacga acggagcaag aagaaactat tgcttctttg caaatggaag 601 tctgtagatt aaaaaaggag gaagaagatc gcattgtcac tcaaaacaga gtgtttgcct 661 atctgtgcaa aagagttcct cataccgtct tggatagaca gttgctttgt ctaattgatt 721 actatgaatc taaaattaga aaaattcata cacaaaggca atataaagaa gatgaaagtc 781 agtcagaaga agaaaacgac tacagaaatc tggatgcctc accaacttat aaaggccttt 841 taatgtcatt acagaatcaa ctcaaggaat caaaatctaa gattgatgca ctttcaagtg 901 aaaagctgaa cctccaaaaa gatttggaaa ccaggcctac gcagcatgaa ttaagacttt 961 ataaacagca ggtgaagaag ctggaaaaag cccttaagaa aaacgtcaaa ttacaggagc 1021 ttattaatca taagaaggct gaggacacag agaagaaaga tgagcccagc aaatataatc 1081 agcaacaggc cctaattgac cagagatact ttcaggtgct gtgtagcatc aattcaatta 1141 tccacaatcc aagagctcca gtaataattt ataaacagac caaaggggga gtccaaaatt 1201 ttaataaaga tcttgttcaa gattgtggat ttgagcatct tgttcctgta atagaaatgt 1261 gggcagatca actgacatcc ttaaaggatt tgtataagtc cttgaaaaca ctatctgcag 1321 aactggtacc ttggcttaat ttgaagaagc aggatgaaaa tgaaggtatc aaagttgaag 1381 atttgttgtt tatagtagat actatgctgg aagaagttga aaataaggaa aaggacagca 1441 atatgcCACA CTTTCAAACT TTGCAAGCTA TTGTTTCTCA CTTCCAAAAG TTATTTGATG 1501 TGCCTTCTTT AAATGGAGTC TATCCCCGAA TGAATGAAGT TTATACTAGG CTTGGAGAAA 1561 TGAACAATGC TGTGAGAAAC CTCCAAGAAC TCTTAGAATT AGATAGTTCA TCCTCATTGT 1621 GTGTGCTAGT AAGCACTGTT GGAAAACTCT GTAGGCTGAT TAATGAAGAT GTGAATGAGC 1681 AGGTTATGCA GGTATTAGGA CCTGAAGACC TCCAGAGCAT TATCTACAAA TTGGAAGAAC 1741 ACGAGGAATT TTTCCCAGCA TTTCAGGCAT TTACTAATGA TCTACTTGAA ATCTTAGAAA 1801 TTGATGACTT GGATGCCATT GTACCTGCAG TAAAGTAATT AAAAGTACTT TCATACTGCC 1861 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1921 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1981 ATATATCTTG TGGAAAGGAC GAAAATTTTC ATGCAATATT GAGCCAACGC GTTAAGTCga 2041 caatcaacct ctggattaca aaatttgtga aagatt