Construct: ORF TRCN0000468045
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018907.3_s317c1
- Derived from:
- ccsbBroadEn_14751
- DNA Barcode:
- TGTTCTGACCCCTTACGGAGCCAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PHKA1 (5255)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468045
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5255 | PHKA1 | phosphorylase kinase regula... | NM_001122670.1 | 99.5% | 20.9% | (many diffs) |
| 2 | human | 5255 | PHKA1 | phosphorylase kinase regula... | NM_002637.4 | 98.5% | 20.7% | (many diffs) |
| 3 | human | 5255 | PHKA1 | phosphorylase kinase regula... | NM_001172436.1 | 94.6% | 22% | (many diffs) |
| 4 | human | 5255 | PHKA1 | phosphorylase kinase regula... | XM_006724661.2 | 93.6% | 21.7% | (many diffs) |
| 5 | human | 5255 | PHKA1 | phosphorylase kinase regula... | XR_001755696.1 | 53.2% | (many diffs) | |
| 6 | mouse | 18679 | Phka1 | phosphorylase kinase alpha 1 | XM_006527894.3 | 89.8% | 20.5% | (many diffs) |
| 7 | mouse | 18679 | Phka1 | phosphorylase kinase alpha 1 | NM_008832.2 | 88.8% | 20.3% | (many diffs) |
| 8 | mouse | 18679 | Phka1 | phosphorylase kinase alpha 1 | NM_173021.3 | 84.4% | 21.3% | (many diffs) |
| 9 | mouse | 18679 | Phka1 | phosphorylase kinase alpha 1 | XM_006527895.3 | 83.1% | 21.7% | (many diffs) |
| 10 | mouse | 18679 | Phka1 | phosphorylase kinase alpha 1 | XM_017318427.1 | 60.5% | 31.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 879
- ORF length:
- 810
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaggagccgg agtaactccg gggtccggct ggacggctac gctcgactgg 121 tgcaacagac catcctgtgc catcagaatc cagtgactgg cttgcttcca gccagctatg 181 atcagaaaga tgcttgggtc cgagataatg tgtacagcat cttggctgtg tggggtttgg 241 gcctggccta tcggaagaat gcagaccggg atgaggataa ggcaaaggcc tatgaattgg 301 agcagagtgt agtgaagctg atgagaggac tactgcactg catgatcaga caggtggata 361 aagtagaatc cttcaaatat agtcagagta ctaaggatag cctccatgca aagtacaaca 421 ccaaaacctg tgccactgta gtgggtgatg atcaatgggg acacctgcag ttggatgcta 481 cctctgtgta cctgctcttc ttagcccaaa tgactgcctc aggactccat atcatccaca 541 gcctagatga agtcaatttc atacagaacc ttgtgtttta cattgaagct gcatataaaa 601 ctgctgactt cgggatatgg gaacgtggag acaagaccaa ccaagggatc tcagagttga 661 atgccagttc agttggaatg gcaaaggcag ccctggaagc attagatgaa ctggatctgt 721 ttggtgtgaa aggtgggcct caatcagtta tccatgtcct ggctgatgaa gtacagcact 781 gccagtctat cctaaattca ctactgcccc gtgcttcaca tcaaagagtg atgctagtct 841 actctcagtg gtttccttcc ctgccttgca gtagagggta gccagttggt ggagctcaca 901 aaacaggaaa tcatcaccaa gcttcagggt cgttatggtt gctgtcgctt tctacgagat 961 ggatataaaa ctcctaaaga ggatcccaat cgtctgtact atgaaccagc tgagctgaag 1021 ctatttgaaa acattgagtg tgaatggcca ttgttctgga catactttat tcttgatggg 1081 gtcttcagtg gcaatgcaga acaggttcaa gaatataaag aggctcttga agcagtcctc 1141 atcaagggca aaaatggagt cccacttctg ccagagctgt acagtgttcc tcctgacagg 1201 gtcgatgaag aatatcagaa tcctcacact gtggaccgag tccccatggg gaaattgcct 1261 cacatgtggg gtcagtctct atacatttta ggaagcttga tggcagaggg atttttagcc 1321 cctggagaaa ttgatcccct gaatcgcagg ttttctactg taccgaagcc cgatgttgtg 1381 gttcaagtct ccattctagc tgaaacagaa gaaatcaaga ccattttgaa ggacaaggga 1441 atttacgtgg agaccattgc tgaggtatac cccatcagag tacaaccagc tcgtattctc 1501 agccacattt attccagcct aggatgcaac aatagaatga aactcagtgg acgaccctac 1561 agacacatgg gagtgcttgg aacttcaaaa ctctatgaca ttcggaaaac tatctttact 1621 ttcactccac agtttataga ccagcaacag ttctacctgg ctctggacaa caagatgata 1681 gtggaaatgc ttagaacaga cctctcctac ctctgtagcc gctggcggat gacaggccag 1741 cccaccatca ccttccccat ctcatacagc atgcttgatg aagatggaac aagcttgaat 1801 tcaagtatcc tggcagcact ccgaaaaatg caagatgggt attttggtgg ggcaagggtt 1861 caaacaggta aattgtcaga gtttttgaca acatcttgtt gcacacactt gagcttcatg 1921 gaccctggac ctgagggtaa gctgtacagt gaagattatg atgacaacta tgattacctg 1981 gaatctggca actggatgaa tgattatgat tcaaccagtc atgctcgctg tggtgatgaa 2041 gttgctcgtt atttagatca ccttttggcg cacactgctc cccatcctaa actagcccct 2101 acctcacaga agggagggct agatcggttc caagctgctg tgcaaacaac ctgcgactta 2161 atgtccttgg tgaccaaggc caaggaactg catgtacaga atgttcacat gtatcttcct 2221 acgaagttat ttcaggcttc ccggccttca ttcaacttac ttgattcacc tcatccccga 2281 caggagaacc aggttccctc tgttcgtgta gaaatacatc ttcctagaga ccagtctggg 2341 gaggtggact ttaaagcact ggttttacag ttgaaggaga cctcaagctt acaggaacaa 2401 gctgatatcc tctatatgct gtatactatg aaaggacctg actggaacac tgaattgtat 2461 aatgaacgga gtgctacagt gagagagctt cttaccgagc tgtatggcaa agtgggagaa 2521 attcgtcact ggggcctgat ccgatacatt tctgggatct taaggaagaa agtggaagca 2581 cttgatgagg cctgcacaga ccttctctcc caccagaaac atttgacagt aggacttcct 2641 ccagaacctc gagaaaagac tatctctgca cctctgccct atgaggcgct cactcagctg 2701 atagatgaag ccagtgaagg ggatatgagc atttcaatcc ttacacagga aataatggta 2761 tatctagcca tgtatatgcg aacccagcct ggcctctttg ctgaaatgtt tcgacttcga 2821 attggtctga tcatacaagt tatggcaaca gaactggccc actcccttcg atgctcagct 2881 gaggaagcca cagagggcct gatgaatctc agtccttcgc ccatgaagga tctcctgcat 2941 cacattctca gccgcaagga gttggagtgg gaacgaagcg ttcgtcccac tgatcaaaat 3001 gtcagtcctg ctatttctat ccacgagatt ggtgctgtgg agcaaccaaa acagaacgaa 3061 ctgggatcat gcagttaaaa agtgagataa agcagtcacc tggaacctct atgactccaa 3121 gtagtgggtc ctttcctagt gcatatgatc agcagtcatc taaagatagt cgtcaaggtc 3181 aatggcaacg ccgaagaagg ctggatgggg cactgaatag agttccagtt ggattttatc 3241 agaaagtatg gaaagttttg cagaagtgtc acggactttc tgttgaaggg tttgtccttc 3301 cttcctctac cactagagag atgactccag gtgagattaa attctctgtt catgtggagt 3361 ctgtcctgaa tcgtgtaccT CAGCCAGAGT ACCGTCAGCT GCTGGTTGAA GCCATCCTTG 3421 TCCTCACCAT GCTGGCAGAT ATTGAAATTC ATAGCATCGG AAGCATCATT GCTGTGGAAA 3481 AAATAGTGCA TATTGCCAAT GACTTGTTCC TTCAAGAACA GAAAACCCTT GGCGCAGATG 3541 ATACCATGTT GGCAAAGGAT CCCGCATCTG GCATCTGTAC TCTTCTGTAT GACAGTGCAC 3601 CCAGTGGCAG GTTTGGCACC ATGACCTACC TCTCCAAGGC AGCCGCCACC TACGTGCAGG 3661 AGTTCCTGCC TCACAGCATC TGTGCCATGC AATTGCCAAC TTTCTTGTAC AAAGTGGTTG 3721 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 3781 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATG 3841 TTCTGACCCC TTACGGAGCC ACACGCGTTA AGTCgacaat caacctctgg attacaaaat 3901 ttgtgaaaga tt