Construct: ORF ccsbBroadEn_14751
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018907.1_s300c1, BRDN0000458203
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- PHKA1 (5255)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5255 | PHKA1 | phosphorylase kinase regula... | NM_001122670.1 | 99.5% | 20.9% | (many diffs) |
| 2 | human | 5255 | PHKA1 | phosphorylase kinase regula... | NM_002637.4 | 98.5% | 20.7% | (many diffs) |
| 3 | human | 5255 | PHKA1 | phosphorylase kinase regula... | NM_001172436.1 | 94.6% | 22% | (many diffs) |
| 4 | human | 5255 | PHKA1 | phosphorylase kinase regula... | XM_006724661.2 | 93.6% | 21.7% | (many diffs) |
| 5 | human | 5255 | PHKA1 | phosphorylase kinase regula... | XR_001755696.1 | 53.2% | (many diffs) | |
| 6 | mouse | 18679 | Phka1 | phosphorylase kinase alpha 1 | XM_006527894.3 | 89.8% | 20.5% | (many diffs) |
| 7 | mouse | 18679 | Phka1 | phosphorylase kinase alpha 1 | NM_008832.2 | 88.8% | 20.3% | (many diffs) |
| 8 | mouse | 18679 | Phka1 | phosphorylase kinase alpha 1 | NM_173021.3 | 84.4% | 21.3% | (many diffs) |
| 9 | mouse | 18679 | Phka1 | phosphorylase kinase alpha 1 | XM_006527895.3 | 83.1% | 21.7% | (many diffs) |
| 10 | mouse | 18679 | Phka1 | phosphorylase kinase alpha 1 | XM_017318427.1 | 60.5% | 31.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 98
- ORF end:
- 908
- ORF length:
- 810
- Sequence:
-
1 AATGATTTTA TTTTGACTGA TAGTGACCTG TTCGTTGCAA CAAATTGATG AGCAATGCTT 61 TTTTATAATG CCAACTTTGT ACAAAAAAGT TGGCACCATG AGGAGCCGGA GTAACTCCGG 121 GGTCCGGCTG GACGGCTACG CTCGACTGGT GCAACAGACC ATCCTGTGCC ATCAGAATCC 181 AGTGACTGGC TTGCTTCCAG CCAGCTATGA TCAGAAAGAT GCTTGGGTCC GAGATAATGT 241 GTACAGCATC TTGGCTGTGT GGGGTTTGGG CCTGGCCTAT CGGAAGAATG CAGACCGGGA 301 TGAGGATAAG GCAAAGGCCT ATGAATTGGA GCAGAGTGTA GTGAAGCTGA TGAGAGGACT 361 ACTGCACTGC ATGATCAGAC AGGTGGATAA AGTAGAATCC TTCAAATATA GTCAGAGTAC 421 TAAGGATAGC CTCCATGCAA AGTACAACAC CAAAACCTGT GCCACTGTAG TGGGTGATGA 481 TCAATGGGGA CACCTGCAGT TGGATGCTAC CTCTGTGTAC CTGCTCTTCT TAGCCCAAAT 541 GACTGCCTCA GGACTCCATA TCATCCACAG CCTAGATGAA GTCAATTTCA TACAGAACCT 601 TGTGTTTTAC ATTGAAGCTG CATATAAAAC TGCTGACTTC GGGATATGGG AACGTGGAGA 661 CAAGACCAAC CAAGGGATCT CAGAGTTGAA TGCCAGTTCA GTTGGAATGG CAAAGGCAGC 721 CCTGGAAGCA TTAGATGAAC TGGATCTGTT TGGTGTGAAA GGTGGGCCTC AATCAGTTAT 781 CCATGTCCTG GCTGATGAAG TACAGCACTG CCAGTCTATC CTAAATTCAC TACTGCCCCG 841 TGCTTCACAT CAAAGAGTGA TGCTAGTCTA CTCTCAGTGG TTTCCTTCCC TGCCTTGCAG 901 TAGAGGGtag ccagttggtg gagctcacaa aacaggaaat catcaccaag cttcagggtc 961 gttatggttg ctgtcgcttt ctacgagatg gatataaaac tcctaaagag gatcccaatc 1021 gtctgtacta tgaaccagct gagctgaagc tatttgaaaa cattgagtgt gaatggccat 1081 tgttctggac atactttatt cttgatgggg tcttcagtgg caatgcagaa caggttcaag 1141 aatataaaga ggctcttgaa gcagtcctca tcaagggcaa aaatggagtc ccacttctgc 1201 cagagctgta cagtgttcct cctgacaggg tcgatgaaga atatcagaat cctcacactg 1261 tggaccgagt ccccatgggg aaattgcctc acatgtgggg tcagtctcta tacattttag 1321 gaagcttgat ggcagaggga tttttagccc ctggagaaat tgatcccctg aatcgcaggt 1381 tttctactgt accgaagccc gatgttgtgg ttcaagtctc cattctagct gaaacagaag 1441 aaatcaagac cattttgaag gacaagggaa tttacgtgga gaccattgct gaggtatacc 1501 ccatcagagt acaaccagct cgtattctca gccacattta ttccagccta ggatgcaaca 1561 atagaatgaa actcagtgga cgaccctaca gacacatggg agtgcttgga acttcaaaac 1621 tctatgacat tcggaaaact atctttactt tcactccaca gtttatagac cagcaacagt 1681 tctacctggc tctggacaac aagatgatag tggaaatgct tagaacagac ctctcctacc 1741 tctgtagccg ctggcggatg acaggccagc ccaccatcac cttccccatc tcatacagca 1801 tgcttgatga agatggaaca agcttgaatt caagtatcct ggcagcactc cgaaaaatgc 1861 aagatgggta ttttggtggg gcaagggttc aaacaggtaa attgtcagag tttttgacaa 1921 catcttgttg cacacacttg agcttcatgg accctggacc tgagggtaag ctgtacagtg 1981 aagattatga tgacaactat gattacctgg aatctggcaa ctggatgaat gattatgatt 2041 caaccagtca tgctcgctgt ggtgatgaag ttgctcgtta tttagatcac cttttggcgc 2101 acactgctcc ccatcctaaa ctagccccta cctcacagaa gggagggcta gatcggttcc 2161 aagctgctgt gcaaacaacc tgcgacttaa tgtccttggt gaccaaggcc aaggaactgc 2221 atgtacagaa tgttcacatg tatcttccta cgaagttatt tcaggcttcc cggccttcat 2281 tcaacttact tgattcacct catccccgac aggagaacca ggttccctct gttcgtgtag 2341 aaatacatct tcctagagac cagtctgggg aggtggactt taaagcactg gttttacagt 2401 tgaaggagac ctcaagctta caggaacaag ctgatatcct ctatatgctg tatactatga 2461 aaggacctga ctggaacact gaattgtata atgaacggag tgctacagtg agagagcttc 2521 ttaccgagct gtatggcaaa gtgggagaaa ttcgtcactg gggcctgatc cgatacattt 2581 ctgggatctt aaggaagaaa gtggaagcac ttgatgaggc ctgcacagac cttctctccc 2641 accagaaaca tttgacagta ggacttcctc cagaacctcg agaaaagact atctctgcac 2701 ctctgcccta tgaggcgctc actcagctga tagatgaagc cagtgaaggg gatatgagca 2761 tttcaatcct tacacaggaa ataatggtat atctagccat gtatatgcga acccagcctg 2821 gcctctttgc tgaaatgttt cgacttcgaa ttggtctgat catacaagtt atggcaacag 2881 aactggccca ctcccttcga tgctcagctg aggaagccac agagggcctG ATGAATCTCA 2941 GTCCTTCGCC CATGAAGGAT CTCCTGCATC ACATTCTCAG CCGCAAGGAG TTGGAGTGGG 3001 AACGAAGCGT TCGTCCCACT GATCAAAATG TCAGTCCTGC TATTTCTATC CACGAGATTG 3061 GTGCTGTGGA GCAACCAAAA CAGAACGAAC TGGGATCATG CAGTTAAAAA GTGAGATAAA 3121 GCAGTCACCT GGAACCTCTA TGACTCCAAG TAGTGGGTCC TTTCCTAGTG CATATGATCA 3181 GCAGTCATCT AAAGATAGTC GTCAAGGTCA ATGGCAACGC CGAAGAAGGC TGGATGGGGC 3241 ACTGAATAGA GTTCCAGTTG GATTTTATCA GAAAGTATGG AAAGTTTTGC AGAAGTGTCA 3301 CGGACTTTCT GTTGAAGGGT TTGTCCTTCC TTCCTCTACC ACTAGAGAGA TGACTCCAGG 3361 TGAGATTAAA TTCTCTGTTC ATGTGGAGTC TGTCCTGAAT CGTGTACCTC AGCCAGAGTA 3421 CCGTCAGCTG CTGGTTGAAG CCATCCTTGT CCTCACCATG CTGGCAGATA TTGAAATTCA 3481 TAGCATCGGA AGCATCATTG CTGTGGAAAA AATAGTGCAT ATTGCCAATG ACTTGTTCCT 3541 TCAAGAACAG AAAACCCTTG GCGCAGATGA TACCATGTTG GCAAAGGATC CCGCATCTGG 3601 CATCTGTACT CTTCTGTATG ACAGTGCACC CAGTGGCAGG TTTGGCACCA TGACCTACCT 3661 CTCCAAGGCA GCCGCCACCT ACGTGCAGGA GTTCCTGCCT CACAGCATCT GTGCCATGCA 3721 ATTGCCAACT TTCTTGTACA AAGTTGGCAT TATAAGAAAG CATTGCTTAT CAATTTGTTG 3781 CAACGAACAG GTCACTATCA GTCAAAATAA AATCATTATT GCCATCCAGG CTGATTCCCC 3841 CGT