Transcript: Human NM_002637.4

Homo sapiens phosphorylase kinase regulatory subunit alpha 1 (PHKA1), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
PHKA1 (5255)
Length:
6286
CDS:
427..4098

Additional Resources:

NCBI RefSeq record:
NM_002637.4
NBCI Gene record:
PHKA1 (5255)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

Clone ID Target Seq Vector PAM Seq Cut Position Cut % of CDS Length Exon On Target Score[?] Other Matching Genes Orig. Target Gene[?] Notes Addgene[?]
1 BRDN0001144804 TGGACGACCCTACAGACACA pXPR_003 TGG 1501 41% 15 0.4709 PHKA1 PHKA1 77839
2 BRDN0001148113 GTAGGGGCTAGTTTAGGATG pXPR_003 GGG 2022 55% 19 0.3725 PHKA1 PHKA1 77841
3 BRDN0001144977 GGGAAATTGCCTCACATGTG pXPR_003 GGG 1202 33% 12 0.3185 PHKA1 PHKA1 77838
4 BRDN0001145492 ATGTATTTCTACACGAACAG pXPR_003 AGG 2239 61% 21 -0.5650 PHKA1 PHKA1 77840
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shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_002637.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000194762 CAAGCTGATATCCTCTATATG pLKO.1 2761 CDS 100% 13.200 18.480 N PHKA1 n/a
2 TRCN0000197239 GCAAACAACCTGCGACTTAAT pLKO.1 2505 CDS 100% 13.200 18.480 N PHKA1 n/a
3 TRCN0000196868 GTGATGAAGTTGCTCGTTATT pLKO.1 2396 CDS 100% 13.200 18.480 N PHKA1 n/a
4 TRCN0000322015 GTTCGTCCCACTGATTCAAAT pLKO_005 3343 CDS 100% 13.200 18.480 N Phka1 n/a
5 TRCN0000342713 GTTCGTCCCACTGATTCAAAT pLKO_005 3343 CDS 100% 13.200 18.480 N PHKA1 n/a
6 TRCN0000006188 GCTATTTCTATCCACGAGATT pLKO.1 3373 CDS 100% 4.950 6.930 N PHKA1 n/a
7 TRCN0000199840 CGGTTGATTGTGCATGGAACT pLKO.1 4149 3UTR 100% 4.050 5.670 N PHKA1 n/a
8 TRCN0000342703 CGGTTGATTGTGCATGGAACT pLKO_005 4149 3UTR 100% 4.050 5.670 N PHKA1 n/a
9 TRCN0000006190 GCTCGTTATTTAGATCACCTT pLKO.1 2407 CDS 100% 2.640 3.696 N PHKA1 n/a
10 TRCN0000196578 GTTATTGCCTAATCACTCCAA pLKO.1 4175 3UTR 100% 2.640 3.696 N PHKA1 n/a
11 TRCN0000342773 GTTATTGCCTAATCACTCCAA pLKO_005 4175 3UTR 100% 2.640 3.696 N PHKA1 n/a
12 TRCN0000342774 TGATGAAGTTGCTCGTTATTT pLKO_005 2397 CDS 100% 15.000 10.500 N PHKA1 n/a
13 TRCN0000199322 CCAGCCTAGGATGCAACAATA pLKO.1 1877 CDS 100% 13.200 9.240 N PHKA1 n/a
14 TRCN0000199049 CCAGCTGAGCTGAAGCTATTT pLKO.1 1369 CDS 100% 13.200 9.240 N PHKA1 n/a
15 TRCN0000342702 CCAGCTGAGCTGAAGCTATTT pLKO_005 1369 CDS 100% 13.200 9.240 N PHKA1 n/a
16 TRCN0000199346 CTCACCATGCTGGCAGATATT pLKO.1 3826 CDS 100% 13.200 9.240 N PHKA1 n/a
17 TRCN0000052610 CCTGCTCTTCTTAGCCCAAAT pLKO.1 849 CDS 100% 10.800 7.560 N PHKA1P1 n/a
18 TRCN0000199512 GCACTGCCAGTCTATCCTAAA pLKO.1 1134 CDS 100% 10.800 7.560 N PHKA1 n/a
19 TRCN0000196619 GTAGAAGAAGTGAACACTTAC pLKO.1 4258 3UTR 100% 10.800 7.560 N PHKA1 n/a
20 TRCN0000025071 GCCATTGTTCTGGACATACTT pLKO.1 1410 CDS 100% 5.625 3.938 N Phka1 n/a
21 TRCN0000322011 GCCATTGTTCTGGACATACTT pLKO_005 1410 CDS 100% 5.625 3.938 N Phka1 n/a
22 TRCN0000006187 CCAGTGACAATCTTATGAGTA pLKO.1 4300 3UTR 100% 4.950 3.465 N PHKA1 n/a
23 TRCN0000052609 CCTTCAAATATAGTCAGAGTA pLKO.1 728 CDS 100% 4.950 3.465 N PHKA1P1 n/a
24 TRCN0000052612 GCAGAGTGTAGTGAAGCTGAT pLKO.1 660 CDS 100% 4.050 2.835 N PHKA1P1 n/a
25 TRCN0000006189 CCACAGTTTATAGACCAGCAA pLKO.1 1990 CDS 100% 2.640 1.848 N PHKA1 n/a
26 TRCN0000052611 CCATGCAAAGTACAACACCAA pLKO.1 762 CDS 100% 2.640 1.848 N PHKA1P1 n/a
27 TRCN0000006191 GCCTGATGAATCTCAGTCCTT pLKO.1 3260 CDS 100% 2.640 1.848 N PHKA1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_002637.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14751 pDONR223 44.5% 98.5% 20.7% None (many diffs) n/a
2 ccsbBroad304_14751 pLX_304 0% 98.5% 20.7% V5 (not translated due to prior stop codon) (many diffs) n/a
3 TRCN0000468045 TGTTCTGACCCCTTACGGAGCCAC pLX_317 8.8% 98.5% 20.7% V5 (not translated due to prior stop codon) (many diffs) n/a
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