Construct: ORF TRCN0000468046
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018793.2_s317c1
- Derived from:
- ccsbBroadEn_15340
- DNA Barcode:
- ACGTATCCAGTATGTTCTCCACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC653155 (653155)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468046
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NM_003913.5 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 2 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011410.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 3 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011411.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 4 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011413.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 5 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011414.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 6 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011415.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 7 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011416.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 8 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011417.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 9 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011418.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 10 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011420.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 11 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011421.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 12 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011422.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 13 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011423.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 14 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011424.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 15 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011425.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 16 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011426.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 17 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011427.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 18 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011428.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 19 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | XM_017011430.2 | 10.4% | 10.5% | 1_2703del;3000C>T |
| 20 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146784.2 | 8.8% | 1_2829del;3126C>T;3148_3582del | |
| 21 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146783.2 | 8.5% | 1_2829del;3126C>T;3148_3715del | |
| 22 | human | 8899 | PRPF4B | pre-mRNA processing factor 4B | NR_146785.2 | 8.3% | 1_2829del;3126C>T;3148_3792del | |
| 23 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | NM_013830.2 | 9.8% | 10.5% | (many diffs) |
| 24 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315425.1 | 9.8% | 10.5% | (many diffs) |
| 25 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315426.1 | 9.8% | 10.5% | (many diffs) |
| 26 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315427.1 | 9.8% | 10.5% | (many diffs) |
| 27 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315428.1 | 9.8% | 10.5% | (many diffs) |
| 28 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315429.1 | 9.8% | 10.5% | (many diffs) |
| 29 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315430.1 | 9.8% | 10.5% | (many diffs) |
| 30 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315431.1 | 9.8% | 10.5% | (many diffs) |
| 31 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315432.1 | 9.8% | 10.5% | (many diffs) |
| 32 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315433.1 | 9.8% | 10.5% | (many diffs) |
| 33 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315434.1 | 9.8% | 10.5% | (many diffs) |
| 34 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315435.1 | 9.8% | 10.5% | (many diffs) |
| 35 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315436.1 | 9.8% | 10.5% | (many diffs) |
| 36 | mouse | 19134 | Prpf4b | pre-mRNA processing factor 4B | XM_017315437.1 | 9.8% | 10.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 387
- ORF length:
- 318
- Sequence:
-
1 tcttccatTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCACCAT GGATCTCAAA GGAAAGATGC CAAATAAGAT GATTCGAAAA GGTGTGTTCA 121 AAGATCAGCA TTTTGATCAA AATCTCAACT TCATGTACAT AGAAGTTGAT AAAGTAACAG 181 AGAGGGAGAA AGTTACTGTT ATGAGCACCA TTAATCCAAC TAAGGACCTG TTGGCTGACT 241 TGATTGGGTG CCAGAGACTT CCTGAAGACC AACGTAAGAA AGTACACCAG CTAAAGGACT 301 TGTTGGACCA GATTCTGATG TTGGACCCAG CTAAACGAAT TAGCATCAAC CAGGCCCTAC 361 AGCATGCCTT CATCCAGGAA AAAATTTTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 421 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 481 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACGTATCC 541 AGTATGTTCT CCACTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 601 aagatt