Construct: ORF TRCN0000468062
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018212.1_s317c1
- Derived from:
- ccsbBroadEn_11024
- DNA Barcode:
- GAATTGTACCGACACCTAGAGGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDGFRA (5156)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468062
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347827.2 | 26.4% | 26% | (many diffs) |
| 2 | human | 5156 | PDGFRA | platelet derived growth fac... | XM_017008281.1 | 25.9% | 25.6% | (many diffs) |
| 3 | human | 5156 | PDGFRA | platelet derived growth fac... | XM_006714041.3 | 25.6% | 25.2% | (many diffs) |
| 4 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347829.2 | 19.5% | 19.2% | (many diffs) |
| 5 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_006206.6 | 19.5% | 19.2% | (many diffs) |
| 6 | human | 5156 | PDGFRA | platelet derived growth fac... | XM_005265743.1 | 19.5% | 19.2% | (many diffs) |
| 7 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347830.1 | 19.3% | 19% | (many diffs) |
| 8 | human | 5156 | PDGFRA | platelet derived growth fac... | NM_001347828.2 | 19.1% | 18.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg gacttcccat ccggcgttcc tggtcttagg ctgtcttctc acagggctga 121 gcctaatcct ctgccagctt tcattaccct ctatccttcc aaatgaaaat gaaaaggttg 181 tgcagctgaa ttcatccttt tctctgagat gctttgggga gagtgaagtg agctggcagt 241 accccatgtc tgaagaagag agctccgatg tggaaatcag aaatgaagaa aacaacagcg 301 gcctttttgt gacggtcttg gaagtgagca gtgccTCGGC GGCCCACACA GGGTTGTACA 361 CTTGCTATTA CAACCACACT CAGACAGAAG AGAATGAGCT TGAAGGCAGG CACATTTACA 421 TCTATGTGCC AGACCCAGAT GTAGCCTTTG TACCTCTAGG AATGACGGAT TATTTAGTCA 481 TCGTGGAGGA TGATGATTCT GCCATTATAC CTTGTCGCAC AACTGATCCC GAGACTCCTG 541 TAACCTTACA CAACAGTGAG GGGGTGGTAC CTGCCTCCTA CGACAGCAGA CAGGGCTTTA 601 ATGGGACCTT CACTGTAGGG CCCTATATCT GTGAGGCCAC CGTCAAAGGA AAGAAGTTCC 661 AGACCATCCC ATTTAATGTT TATGCTTTAA AAGGTACTTG TATCATCTCC TTCCTTCTTT 721 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 841 TTTATATATC TTGTGGAAAG GACGAGAATT GTACCGACAC CTAGAGGACA CGCGTTAAGT 901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt