Construct: ORF TRCN0000468069
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004107.1_s317c1
- Derived from:
- ccsbBroadEn_08609
- DNA Barcode:
- CCTTACAGGTTCCCTTATCCAGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BIN3 (55909)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468069
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55909 | BIN3 | bridging integrator 3 | NM_018688.6 | 99.8% | 100% | 6C>T |
| 2 | human | 55909 | BIN3 | bridging integrator 3 | NM_001363046.1 | 81% | 78.6% | 0_1ins82;16_17ins62 |
| 3 | human | 55909 | BIN3 | bridging integrator 3 | XM_011544586.2 | 78.6% | 78.6% | 0_1ins162 |
| 4 | human | 55909 | BIN3 | bridging integrator 3 | XM_011544587.1 | 53.9% | 47.8% | (many diffs) |
| 5 | human | 55909 | BIN3 | bridging integrator 3 | NR_156436.1 | 43.4% | (many diffs) | |
| 6 | mouse | 57784 | Bin3 | bridging integrator 3 | NM_021328.3 | 87.4% | 94.4% | (many diffs) |
| 7 | mouse | 57784 | Bin3 | bridging integrator 3 | XM_006519330.2 | 76.4% | 73.9% | (many diffs) |
| 8 | mouse | 57784 | Bin3 | bridging integrator 3 | XM_006519331.2 | 72.3% | 77.8% | (many diffs) |
| 9 | mouse | 57784 | Bin3 | bridging integrator 3 | XM_006519332.3 | 72.3% | 77.8% | (many diffs) |
| 10 | mouse | 57784 | Bin3 | bridging integrator 3 | XM_011245124.2 | 45.8% | 49% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 825
- ORF length:
- 759
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ttggattcct tttaagattg ggcagcccaa gaaacagatt gtgcccaaaa 121 cagtggagag agactttgaa agggagtatg gaaaacttca gcagctggaa gagcagaccc 181 ggaggctgca gaaagacatg aagaagagca ccgacgcaga cctggccatg tcaaaatctg 241 ccgtgaagat atccttggac ttactctcca atcccctctg tgagcaagac caggaccttc 301 tgaacatggt gacggccctg gacacggcca tgaagcggat ggatgccttc aatcaggaaa 361 aggtgaacca gatccagaag actgtgatcg agcccttaaa aaagttcggc agtgtcttcc 421 cgagcctcaa catggctgtg aagaggcggg aacaggcctt gcaggactac aggaggctgc 481 aggccaaggt ggagaagtat gaggaaaagg agaagacggg gccagtgctg gccaagctcc 541 accaggcacg agaggagctg cggcctgtgc gggaggactt tgaagccaag aacaggcagc 601 tgctggagga gatgccgcgc ttctacggca gccgcctcga ctacttccag cccagctttg 661 agtccctcat ccgagctcag gttgtgtact actcggaaat gcacaagatc tttggagacc 721 tgtcccatca gcttgaccag ccaggccact ccgatgagca gcgggagcgg gagaacgagg 781 ccaaactcag tgagctccgg gccctctcca ttgtggccga tGACTGCCCA ACTTTCTTGT 841 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 901 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 961 GAAAGGACGA CCTTACAGGT TCCCTTATCC AGAAACGCGT TAAGTCgaca atcaacctct 1021 ggattacaaa atttgtgaaa gatt