Construct: ORF TRCN0000468079
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011865.1_s317c1
- Derived from:
- ccsbBroadEn_13224
- DNA Barcode:
- CTAACGTCTCAGGCCCGACTTGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLT1D1 (144423)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468079
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | XM_017018859.1 | 55.6% | 54.4% | (many diffs) |
| 2 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | NM_144669.3 | 49.6% | 43.2% | (many diffs) |
| 3 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | NM_001366886.1 | 43.9% | 32.4% | 323_374del;509_1038del |
| 4 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | NM_001366889.1 | 41.9% | 30% | 321_420del;557_1086del |
| 5 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | XM_017018855.2 | 38.6% | 27.7% | 1_240del;561_660del;797_1179del |
| 6 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | XR_001748588.1 | 34.4% | 1_259del;580_679del;816_1322del | |
| 7 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | XR_001748591.1 | 34.3% | (many diffs) | |
| 8 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | XR_001748590.1 | 32.3% | (many diffs) | |
| 9 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | NM_001366887.1 | 28.9% | 13% | (many diffs) |
| 10 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | NR_133646.2 | 15.3% | 1_104del;561_2971del | |
| 11 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | XR_001748589.1 | 13.9% | (many diffs) | |
| 12 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | XR_001748587.1 | 13.7% | 1_259del;582_633del;768_3311del | |
| 13 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | XR_001748586.1 | 13.5% | 1_259del;580_679del;816_3359del | |
| 14 | human | 144423 | GLT1D1 | glycosyltransferase 1 domai... | NR_159493.1 | 13.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 522
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gctcctgttc ctggcggtgc tgcggccaca caccggcaac gcggtcacgg 121 cccagcgcgt tcgggcccat ctagaggctg cagggcacgt gtgcgttttg aaggatgcct 181 ttgactttga aagccgatct gagattgcaa accTCATCTT GGCTGAGAAC TGCGAGGCTG 241 CCCTGGCTCT TCATCTCTAT AGGGGAGGCA GGCTTTTGCA AGGCCACCGA ATCCCTTTTG 301 GAGTCATCTT TGGTGGAACT GATGTAAATG AAGATGCCAA CCAGGCGGAA AAAAACACAG 361 TCATGGGCAG AGTTCTTGAG GAAGCCAGCC GCATGCTAAG GGTAAGGTCT ATGTCCAGAG 421 TCAAGGAATT GCAACAACAC CAAACGCCGC TTTTAACTGG AATACCTTTC TTCAACGCTC 481 TGAGATTAAC CAAAGTGCTG ATAATTTGCA CATATTTCTT CTGCCCAACT TTCTTGTACA 541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 661 AGGACGACTA ACGTCTCAGG CCCGACTTGT CACGCGTTAA GTCgacaatc aacctctgga 721 ttacaaaatt tgtgaaagat t