Construct: ORF TRCN0000468117
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018341.1_s317c1
- Derived from:
- ccsbBroadEn_02618
- DNA Barcode:
- ATCGTACAAGACCGAACAACTTTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DIDO1 (11083)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468117
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11083 | DIDO1 | death inducer-obliterator 1 | NM_022105.4 | 100% | 100% | |
| 2 | human | 11083 | DIDO1 | death inducer-obliterator 1 | NM_080796.3 | 100% | 100% | |
| 3 | human | 11083 | DIDO1 | death inducer-obliterator 1 | NM_001193370.2 | 46.5% | 44.8% | (many diffs) |
| 4 | human | 11083 | DIDO1 | death inducer-obliterator 1 | NM_080797.4 | 46.5% | 44.8% | (many diffs) |
| 5 | human | 11083 | DIDO1 | death inducer-obliterator 1 | NM_001193369.2 | 24.7% | 23.8% | (many diffs) |
| 6 | human | 11083 | DIDO1 | death inducer-obliterator 1 | NM_033081.2 | 24.7% | 23.8% | (many diffs) |
| 7 | human | 11083 | DIDO1 | death inducer-obliterator 1 | XM_011528509.2 | 24.7% | 23.8% | (many diffs) |
| 8 | human | 11083 | DIDO1 | death inducer-obliterator 1 | XM_011528504.1 | 24.3% | 23.4% | (many diffs) |
| 9 | human | 11083 | DIDO1 | death inducer-obliterator 1 | XM_011528505.2 | 24.3% | 23.4% | (many diffs) |
| 10 | human | 11083 | DIDO1 | death inducer-obliterator 1 | XM_011528507.1 | 24.3% | 23.4% | (many diffs) |
| 11 | human | 11083 | DIDO1 | death inducer-obliterator 1 | XM_011528508.1 | 24.3% | 23.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1752
- ORF length:
- 1686
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgacaaaggc gacccgagca atgaggaggc acctaaggcc atcaaaccca 121 ccagcaaaga gttcaggaaa acatggggtt ttcgaaggac cactatcgcc aagcgagagg 181 gcgcagggga cgcggaggct gacccactgg agccgccacc cccacagcag cagctgggcc 241 tgtccctgcg gcgcagtggg aggcagccca agcgcactga gcgcgtggag cagttcctga 301 ccattgcgcg gcgccgcggc aggaggagca tgcctgtctc cctggaggat tctggtgagc 361 ccacgtcctg ccccgccaca gacgccgaga cagcctccga gggcagcgtg gaaagcgctt 421 ctgagaccag aagcggcccc cagtctgctt ccacagctgt gaaggaacga ccagcctctt 481 ctgaaaaggt gaaaggaggg gatgaccacg atgacacctc cgatagtgac agcgatggcc 541 tgaccttgaa agagcttcag aatcgccttc gcaggaagcg ggaacaggag cccactgaga 601 ggcccctgaa agggatccag agtcgcctgc ggaagaagcg ccgggaggag ggtcccgccg 661 agactgtggg ctccgaggcc agtgacactg tggagggcgt cctgcccagt aagcaggagc 721 ccgagaacga tcagggggtt gtgtcccagg ctgggaaaga tgacagagag agtaagttgg 781 agggaaaggc ggctcaggac atcaaagatg aggagcctgg agacttgggc cgaccgaagc 841 ctgaatgtga gggttacgac cccaacgccc tgtattgcat ttgccgccag cctcacaaca 901 acaggtttat gatttgctgt gaccgctgtg aagaatggtt tcatggcgat tgtgtgggca 961 tttctgaggc tcgagggagg cttttggaaa ggaatgggga agactatatc tgcccaaact 1021 gcaccattct gcaagtgcag gatgagactc attcagaaac ggcagatcag caggaagcta 1081 aatggagacc tggagatgct gatggcaccg attgtacaag tataggaaca atagagcaga 1141 agtctagcga agaccaaggg ataaagggta gaattgagaa agctgcaaat ccaagtggca 1201 agaagaaact caagatcttc cagcctgtga tagaggcgcc tggtgcctca aaatgtattg 1261 gccccgggtg ctgtcacgtg gcgcagcccg actcggtgta ctgcagtaat gactgtatcc 1321 TCAAACACGC CGCAGCGACA ATGAAGTTTC TAAGCTCAGG TAAAGAACAG AAGCCAAAGC 1381 CTAAAGAAAA GATGAAGATG AAGCCAGAGA AGCCCAGTCT TCCGAAATGC GGTGCTCAGG 1441 CAGGTATTAA AATCTCTTCT GTGCACAAGA GACCAGCTCC AGAAAAAAAA GAGACCACAG 1501 TGAAGAAGGC AGTGGTGGTC CCTGCGCGGA GTGAAGCACT CGGGAAGGAA GCAGCTTGTG 1561 AGAGCAGCAC GCCGTCGTGG GCGAGCGATC ACAATTACAA TGCAGTAAAG CCAGAAAAGA 1621 CTGCTGCTCC CTCGCCGTCA CTGTTGTATA AATGTATGTA TCACCTAGGG GTTGGCCTCC 1681 TGGACCCCTC CCGTTCTTTC TGGATAGCCA TCCCCTGGGC CTGTCCAGGA CTGGGAGTTG 1741 CAGCTTTGTG TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1801 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1861 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAATC GTACAAGACC GAACAACTTT 1921 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t