Construct: ORF TRCN0000468125
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001569.1_s317c1
- Derived from:
- ccsbBroadEn_13714
- DNA Barcode:
- TCGTCTTGCTGTGCCGCTCTGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC554223 (554223)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468125
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 352962 | HLA-V | major histocompatibility co... | NR_132323.1 | 43.7% | 1_102del;670_1295del | |
| 2 | human | 3135 | HLA-G | major histocompatibility co... | XM_017010817.1 | 31.1% | 25.5% | (many diffs) |
| 3 | human | 3135 | HLA-G | major histocompatibility co... | XM_017010818.1 | 31.1% | 25.5% | (many diffs) |
| 4 | human | 3136 | HLA-H | major histocompatibility co... | NR_001434.4 | 25.4% | (many diffs) | |
| 5 | human | 3135 | HLA-G | major histocompatibility co... | NM_001363567.1 | 25.1% | 19.8% | (many diffs) |
| 6 | human | 3135 | HLA-G | major histocompatibility co... | NM_002127.5 | 24.2% | 19.8% | (many diffs) |
| 7 | human | 3135 | HLA-G | major histocompatibility co... | XM_024446420.1 | 24.2% | 19.8% | (many diffs) |
| 8 | human | 3105 | HLA-A | major histocompatibility co... | NM_002116.8 | 22.5% | 19% | (many diffs) |
| 9 | human | 3105 | HLA-A | major histocompatibility co... | NM_001242758.1 | 22.4% | 18.6% | (many diffs) |
| 10 | human | 3106 | HLA-B | major histocompatibility co... | NM_005514.8 | 22.3% | 18.5% | (many diffs) |
| 11 | human | 3133 | HLA-E | major histocompatibility co... | NM_005516.6 | 21.9% | 18.8% | (many diffs) |
| 12 | human | 3133 | HLA-E | major histocompatibility co... | XM_017010809.2 | 21.8% | 18.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 633
- ORF length:
- 567
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ttggggaggc ccagcgctgg gcattcccca tctttgcagg gtttctcttc 121 tccctctccc aacctgtgtc gggtccttct tcctgggtac tcaccgggct gccccagttc 181 tcactcccat tgagtgtcgg gtttctagag aagccaatca atGTAGCCGC GGTCCCGGTT 241 CTAAAGTTCC CACGCACCCA CCGGGACTCC GATTCTTCCC AGTCGCCGAG GATGGTGTCA 301 TGGCGCCCCG AACCCTGCTT CTGCTGCTCT CGGGGGCCCT GGTCCTGACC CAGACCTGGG 361 CAGGCTTCCA CTCCTTGAGG TATTTCCACA CCACCATGTC CCGGCCCGGC CGCGCGGATC 421 CCCGCTTCCT CTCCGTGGGC GACGTGGACG ACACGCAGTG CGTGCGGCTC GACAGCGACG 481 CCACGAGTCC CAGGATGGAG CCGCGGGCGC CGTGGATGGA GCAGGAGGGG CCGGAATATT 541 GGGAAGAGGA GACAGGGACC GCCAAGGCCA AAGCACAGTT TTACCGAGTG AACCTGCGGA 601 CCCTGAGCGG CTACTACAAC CAGAGTGAGG CCTACCCAAC TTTCTTGTAC AAAGTGGTTG 661 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 721 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATC 781 GTCTTGCTGT GCCGCTCTGC CAACGCGTTA AGTCgacaat caacctctgg attacaaaat 841 ttgtgaaaga tt