Construct: ORF TRCN0000468133
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003957.1_s317c1
- Derived from:
- ccsbBroadEn_00470
- DNA Barcode:
- AAGAGGTCGAGTGTGCGTATGTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DVL2 (1856)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468133
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1856 | DVL2 | dishevelled segment polarit... | NM_004422.3 | 100% | 100% | |
| 2 | human | 1856 | DVL2 | dishevelled segment polarit... | XM_005256502.2 | 99.4% | 99.3% | 508_509insGTGAGCATGGCG |
| 3 | human | 1856 | DVL2 | dishevelled segment polarit... | XR_001752443.2 | 78.9% | (many diffs) | |
| 4 | human | 1856 | DVL2 | dishevelled segment polarit... | XR_001752442.2 | 71.6% | 1_282del;1826_1891del;2557_3083del | |
| 5 | human | 1856 | DVL2 | dishevelled segment polarit... | XR_001752441.2 | 63.6% | (many diffs) | |
| 6 | mouse | 13543 | Dvl2 | dishevelled segment polarit... | NM_007888.3 | 88.1% | 96.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2274
- ORF length:
- 2208
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gggtagcagc actgggggcg gtggggttgg ggagacgaag gtgatttacc 121 acctggatga ggaagagact ccctacctgg tgaagatccc tgtccccgcc gagcgcatca 181 ccctcggcga tttcaagagc gtcctgcagc ggcccgcggg cgccaagtac tttttcaagt 241 ctatggatca ggatttcggg gtggtgaagg aagaaatttc agatgacaac gcccgcctcc 301 cctgcttcaa cggaagggtg gtatcctggc tggtgtcctc agataatccc caacccgaga 361 tggcccctcc agtccatgag cctcgggcag aactggcgcc tccagcccca cctttacctc 421 ctttgccacc cgagaggacc agcggcattg gggactcaag gcctccatcc ttccacccta 481 atgtgtccag cagccatgag aatctggagc ctgagacaga aaccgagtca gtagtgtcac 541 tgaggcggga gcggcctcgc aggagagaca gcagtgagca tggcgctggg ggccacagga 601 ctggtggccc ctcaaggctg gagcgccacc tggccggata cgagagctcc tctaccctca 661 tgaccagcga gctggagagt accagcctgg gggactcgga cgaggaggac accatgagca 721 ggttcagcag ctccacggag cagagcagtg cctcccgcct ccttaagcgc caccggcggc 781 gaaggaagca gaggccaccc cgcctggaga ggacgtcatc cttcagcagc gtcacagatt 841 ccacaatgtc tctcaatatc atcacagtca cgctaaacat ggagaagtac aacttcctgg 901 gtatctccat tgttggccag agcaatgagc ggggagacgg aggcatctac attggctcca 961 tcatgaaggg tggggctgtg gcggccgacg ggcgcattga gccaggggac atgcttttgc 1021 aggtgaatga catgaacttt gagaacatga gcaacgatga cgctgtgcgg gtgctgaggg 1081 acattgtgca caagcctggc cccattgtgc tgactgtggc caagtgctgg gatccctctc 1141 ctcaggccta tttcactctc ccccgaaatg agcccatcca gccaattgac cctgctgcct 1201 gggtgtccca ttccgcggct ctgactggca ccttcccagc ctatccaggt tcctcctcca 1261 tgagcaccat tacatctgga tcgtctttgc ctgatggctg tgaaggccgg ggtctctccg 1321 tccatacgga catggcatcg gtgaccaagg ccatggcagc tccagagtct ggactggaag 1381 tccgggaccg catgtggctc aagatcacca tccctaatgc ctttctgggc tcggatgtgg 1441 ttgactggct ctaccatcac gtggagggct ttcctgagcg gcgggaggcc cgcaagtatg 1501 ccagcgggct gctcaaagca ggcctgatcc gacacaccgt caacaagatc accttctctg 1561 agcagtgcta ttacgtcttc ggagacctca gtggtggctg tgagagctac ctagtcaacc 1621 tgtctctcaa tgacaacgat ggctccagtg gggcttcaga ccaggatacc ctggctcctc 1681 tgcctggggc caccccctgg cccctgctgc ccactttctc ctaccaatac cctgccccac 1741 acccctacag cccgcagcct ccaccctacc atgagctttc atcttacacc tatggtgggg 1801 gcagtgccag cagccagcat agtgagggca gccggagcag tgggtcgaca cggagtgatg 1861 ggggggcagg gcgcacgggG AGGCCCGAGG AGCGGGCCCC CGAGTCCAAG TCCGGCAGTG 1921 GCAGTGAGTC TGAGCCCTCC AGCCGAGGGG GCAGCCTTCG GCGGGGTGGG GAAGCAAGTG 1981 GGACTAGCGA TGGGGGCCCT CCTCCATCCA GAGGCTCAAC TGGGGGTGCC CCTAATCTCC 2041 GAGCCCACCC AGGGCTCCAT CCCTATGGAC CGCCCCCTGG CATGGCCCTC CCCTACAACC 2101 CCATGATGGT GGTCATGATG CCCCCACCTC CACCTCCAGT CCCTCCAGCA GTGCAGCCTC 2161 CGGGGGCCCC TCCAGTCAGA GACCTGGGCT CTGTGCCCCC AGAACTGACA GCCAGCCGCC 2221 AAAGCTTCCA CATGGCCATG GGCAATCCCA GCGAGTTCTT TGTGGATGTT ATGTACCCAA 2281 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2341 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2401 TATCTTGTGG AAAGGACGAA AGAGGTCGAG TGTGCGTATG TTCACGCGTT AAGTCgacaa 2461 tcaacctctg gattacaaaa tttgtgaaag att