Construct: ORF TRCN0000468144
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018871.1_s317c1
- Derived from:
- ccsbBroadEn_14610
- DNA Barcode:
- TACAGTGCAGAGCATGATATCCCG
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DAPK3 (1613)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468144
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1613 | DAPK3 | death associated protein ki... | NM_001348.3 | 98.6% | 41.4% | (many diffs) |
| 2 | human | 1613 | DAPK3 | death associated protein ki... | XM_005259508.2 | 98.6% | 41.4% | (many diffs) |
| 3 | mouse | 13144 | Dapk3 | death-associated protein ki... | NM_001190473.1 | 80% | 39.5% | (many diffs) |
| 4 | mouse | 13144 | Dapk3 | death-associated protein ki... | NM_007828.2 | 80% | 39.5% | (many diffs) |
| 5 | mouse | 13144 | Dapk3 | death-associated protein ki... | XM_006513195.2 | 80% | 39.5% | (many diffs) |
| 6 | mouse | 13144 | Dapk3 | death-associated protein ki... | NM_001190474.1 | 77.2% | 38% | (many diffs) |
| 7 | mouse | 13144 | Dapk3 | death-associated protein ki... | XM_006513194.2 | 75.6% | 38.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 642
- ORF length:
- 573
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtccacgttc aggcaggagg acgtggagga ccattatgag atgggggagg 121 agctgggcag cggccagttt gcgatcgtgc ggaagtgccg gcagaagggc acgggcaagg 181 agtacgcagc caagttcatc aagaagcgcc gcctgtcatc cagccggcgt ggggtgagcc 241 gggaggagat cgagcgggag gtgaacatcc tgcgggagat ccggcacccc aacatcatca 301 ccctgcacga catcttcgag aacaagacgg acgtggtcct catcctggag ctggtctctg 361 gcggggagct ctttgacttc ctggcggaga aggagtcgct gacggaggac gaggccaccc 421 agttcctcaa gcagatcctg gacggcgttc actacctgca ctctaagcgc atcgcacact 481 ttgacctgaa gccggaaaac atcatgctgc tggacaagaa cgtgcccaac ccacgaatca 541 agctcatcga cttcggcatc gcgcacaaga tcgaggcggg gaacgagttc aagaacatct 601 tcggcacccc ggagtttgtg gccccagaga ntgtgaaact atgagccgnt gggcctggag 661 ggnggacatg tggagcatcg gtgtcatcac ctatnttccc tcctgagcng tgcatccccg 721 ttcctgggcg agaccaagca ggagacgctc accaacatct tcagccgtga actacgactt 781 cgacgaggag tacttcagca acaccagcga gctgnccaan gacttcattc gcnngctgct 841 cgtcaaagat cccaagcgga gaatgaccat tgcncagagc ctggaacatt cctggantaa 901 ggcgatccgn cggcggaacg tgcgtggtga ggacagcggc cgcaagcccg agcggcggcg 961 cctgaagacc acgcgtctga aggagtacac catcaagtcg cactccagct tgccgcccaa 1021 caacagctac gccgacttcg agcgcttcTC CAAGGTGCTG GAGGAGGCGG CGGCCGCCGA 1081 GGAGGGCCTG CGCGAGCTGC AGCGCAGCCG GCGGCTCTGC CACGAGGACG TGGAGGCACT 1141 GGCCGCCATC TACGAGGAGA AGGAGGCCTG GTACCGCGAG GAGAGCGACA GCCTGGGCCA 1201 GGACCTGCGG AGGCTACGGC AGGAGCTGCT CAAGACCGAG GCGCTCAAGC GGCAGGCGCA 1261 GGAGGAGGCC AAGGGCGCGC TGCTGGGGAC CAGCGGCCTC AAGCGCCGCT TCAGCCGCCT 1321 GGAGAACCGC TACGAGGCGC TGGCCAAGCA AGTAGCCTCC GAGATGCGCT TCGTGCAGGA 1381 CCTCGTGCGC GCCCTGGAGC AGGAGAAGCT GCAGGGCGTG GAGTGCGGGC TGCGCTTGCC 1441 AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT 1501 CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA 1561 TATATCTTGT GGAAAGGACG ATACAGTGCA GAGCATGATA TCCCGACGCG TTAAGTCgac 1621 aatcaacctc tggattacaa aatttgtgaa agatt