Construct: ORF TRCN0000468155
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003103.1_s317c1
- Derived from:
- ccsbBroadEn_06985
- DNA Barcode:
- TTTTCCTCTAAATTGCCGAATAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SORD (6652)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468155
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6652 | SORD | sorbitol dehydrogenase | NM_003104.6 | 99.9% | 99.7% | 806A>C |
| 2 | human | 653381 | SORD2P | sorbitol dehydrogenase 2, p... | NR_146394.1 | 41.5% | (many diffs) | |
| 3 | human | 653381 | SORD2P | sorbitol dehydrogenase 2, p... | NR_146393.1 | 38% | (many diffs) | |
| 4 | human | 6652 | SORD | sorbitol dehydrogenase | NR_034039.2 | 21.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1137
- ORF length:
- 1071
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggcggcc aagcccaaca acctttccct ggtggtgcac ggaccggggg 121 acttgcgcct ggagaactat cctatccctg aaccaggccc aaatgaggtc ttgctgagga 181 tgcattctgt tggaatctgt ggctcagatg tccactactg ggagtatggt cgaattggga 241 attttattgt gaaaaagccc atggtgctgg gacatgaagc ttcgggaaca gtcgaaaaag 301 tgggatcatc ggtaaagcac ctaaaaccag gtgatcgtgt tgccatcgag cctggtgctc 361 cccgagaaaa tgatgaattc tgcaagatgg gccgatacaa tctgtcacct tccatcttct 421 tctgtgccac gccccccgat gacgggaacc tctgccggtt ctataagcac aatgcagcct 481 tttgttacaa gcttcctgac aatgtcacct ttgaggaagg cgccctgatc gagccacttt 541 ctgtggggat ccatgcctgc aggagaggcg gagttaccct gggacacaag gtccttgtgt 601 gtggagctgg gccaatcggg atggtcactt tgctcgtggc caaagcaatg ggagcagctc 661 aagtagtggt gactgatctg tctgctaccc gattgtccaa agccaaggag attggggctg 721 atttagtcct ccagatctcc aaggagagcc ctcaggaaat cgccaggaaa gtagaaggtc 781 agctggggtg caagccggaa gtcaccatcg agtgcacggg ggcagaggcc tccatccagg 841 cgggcatcta cgccactcgc tctggtggga ccctcgtgct tgtggggctg ggctctgaga 901 tgaccaccgt acccctactg catgcagcca tccgggaggt ggatatCAAG GGCGTGTTTC 961 GATACTGCAA CACGTGGCCA GTGGCGATTT CGATGCTTGC GTCCAAGTCT GTGAATGTAA 1021 AACCCCTCGT CACCCATAGG TTTCCTCTGG AGAAAGCTCT GGAGGCCTTT GAAACATTTA 1081 AAAAGGGATT GGGGTTGAAA ATCATGCTCA AGTGTGACCC CAGTGACCAG AATCCCTGCC 1141 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GATTTTCCTC TAAATTGCCG AATAATACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt