Construct: ORF TRCN0000468159
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010320.1_s317c1
- Derived from:
- ccsbBroadEn_06885
- DNA Barcode:
- CACGAACTCGTGCGTGTGCCACAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL28 (6158)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468159
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6158 | RPL28 | ribosomal protein L28 | NM_000991.5 | 99.7% | 99.2% | 251A>G |
| 2 | human | 6158 | RPL28 | ribosomal protein L28 | NM_001363697.1 | 76.1% | 64.5% | (many diffs) |
| 3 | human | 6158 | RPL28 | ribosomal protein L28 | NM_001136134.1 | 75.6% | 65.1% | (many diffs) |
| 4 | human | 6158 | RPL28 | ribosomal protein L28 | NM_001136135.2 | 75.3% | 63.6% | (many diffs) |
| 5 | human | 6158 | RPL28 | ribosomal protein L28 | NM_001136136.1 | 52.6% | 50.3% | (many diffs) |
| 6 | human | 6158 | RPL28 | ribosomal protein L28 | NM_001136137.1 | 50.3% | 49.6% | 205_206insGCC;207_208ins201 |
| 7 | mouse | 19943 | Rpl28 | ribosomal protein L28 | NM_009081.2 | 89% | 97.8% | (many diffs) |
| 8 | mouse | 100039731 | Gm10051 | predicted pseudogene 10051 | XR_868574.1 | 74.1% | (many diffs) | |
| 9 | mouse | 19943 | Rpl28 | ribosomal protein L28 | XM_006539679.1 | 73.9% | 81.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 477
- ORF length:
- 411
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgcgcatctg caatggatgg tcgtgcggaa ctgctccagt ttcctgatca 121 agaggaataa gcagaccTAC AGCACTGAGC CCAATAACTT GAAGGCCCGC AATTCCTTCC 181 GCTACAACGG ACTGATTCAC CGCAAGACTG TGGGCGTGGA GCCGGCAGCC GACGGCAAAG 241 GTGTCGTGGT GGTCATTAAG CGGAGATCCG GCCAGCGGAA GCCTGCCACC TCCTATGTGC 301 GGACCACCAT CAACAGGAAT GCTCGCGCCA CGCTCAGCAG CATCAGACAC ATGATCCGCA 361 AGAACAAGTA CCGCCCCGAC CTGCGCATGG CAGCCATCCG CAGGGCCAGC GCCATCCTGC 421 GCAGCCAGAA GCCTGTGATG GTGAAGAGGA AGCGGACCCG CCCCACCAAG AGCTCCTGCC 481 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 541 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 601 ATATATCTTG TGGAAAGGAC GACACGAACT CGTGCGTGTG CCACAAACGC GTTAAGTCga 661 caatcaacct ctggattaca aaatttgtga aagatt