Construct: ORF TRCN0000468177
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003844.1_s317c1
- Derived from:
- ccsbBroadEn_04500
- DNA Barcode:
- GCCAGAGGAGTAGCTTTAGACTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLB1L2 (89944)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468177
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89944 | GLB1L2 | galactosidase beta 1 like 2 | NM_001370461.1 | 100% | 100% | |
| 2 | human | 89944 | GLB1L2 | galactosidase beta 1 like 2 | NM_138342.3 | 100% | 100% | |
| 3 | human | 89944 | GLB1L2 | galactosidase beta 1 like 2 | NM_001370460.1 | 92.1% | 92.1% | 732_893del |
| 4 | human | 89944 | GLB1L2 | galactosidase beta 1 like 2 | NM_001370462.1 | 40.8% | 39.3% | (many diffs) |
| 5 | human | 89944 | GLB1L2 | galactosidase beta 1 like 2 | NM_001370463.1 | 24.5% | 23.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1974
- ORF length:
- 1908
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cacgtggagc ctccggcgga ggccggcccg cacgctggga ctcctgctgc 121 tggtcgtctt gggcttcctg gtgctccgca ggctggactg gagcaccctg gtccctctgc 181 ggctccgcca tcgacagctg gggctgcagg ccaagggctg gaacttcatg ctggaggatt 241 ccaccttctg gatcttcggg ggctccatcc actatttccg tgtgcccagg gagtactgga 301 gggaccgcct gctgaagatg aaggcctgtg gcttgaacac cctcaccacc tatgttccgt 361 ggaacctgca tgagccagaa agaggcaaat ttgacttctc tgggaacctg gacctggagg 421 ccttcgtcct gatggccgca gagatcgggc tgtgggtgat tctgcgtcca ggcccctaca 481 tctgcagtga gatggacctc gggggcttgc ccagctggct actccaagac cctggcatga 541 ggctgaggac aacttacaag ggcttcaccg aagcagtgga cctttatttt gaccacctga 601 tgtccagggt ggtgccactc cagtacaagc gtgggggacc tatcattgcc gtgcaggtgg 661 agaatgaata tggttcctat aataaagacc ccgcatacat gccctacgtc aagaaggcac 721 tggaggaccg tggcattgtg gaactgctcc tgacttcaga caacaaggat gggctgagca 781 aggggattgt ccagggagtc ttggccacca tcaacttgca gtcaacacac gagctgcagc 841 tactgaccac ctttctcttc aacgtccagg ggactcagcc caagatggtg atggagtact 901 ggacggggtg gtttgactcg tggggaggcc ctcacaatat cttggattct tctgaggttt 961 tgaaaaccgt gtctgccatt gtggacgccg gctcctccat caacctctac atgttccacg 1021 gaggcaccaa ctttggcttc atgaatggag ccatgcactt ccatgactac aagtcagatg 1081 tcaccagcta tgactatgat gctgtgctga cagaagccgg cgattacacg gccaagtaca 1141 tgaagcttcg agacttcttc ggctccatct caggcatccc tctccctccc ccacctgacc 1201 ttcttcccaa gatgccgtat gagcccttaa cgccagtctt gtacctgtct ctgtgggacg 1261 ccctcaagta cctgggggag ccaatcaagt ctgaaaagcc catcaacatg gagaacctgc 1321 cagtcaatgg gggaaatgga cagtccttcg ggtacattct ctatgagacc agcatcacct 1381 cgtctggcat cctcagtggc cacgtgcatg atcgggggca ggtgtttgtg aacacagtat 1441 ccataggatt cttggactac aagacaacga agattgctgt ccccctgatc cagggttaca 1501 ccgtgctgag gatcttggtg gagaatcgtg ggcgagtcaa ctatggggaG AATATTGATG 1561 ACCAGCGCAA AGGCTTAATT GGAAATCTCT ATCTGAATGA TTCACCCCTG AAAAACTTCA 1621 GAATCTATAG CCTGGATATG AAGAAGAGCT TCTTTCAGAG GTTCGGCCTG GACAAATGGA 1681 GTTCCCTCCC AGAAACACCC ACATTACCTG CTTTCTTCTT GGGTAGCTTG TCCATCAGCT 1741 CCACCCCTTG TGACACCTTT CTGAAGCTGG AGGGCTGGGA GAAGGGGGTT GTATTCATCA 1801 ATGGCCAGAA CCTTGGACGT TACTGGAACA TTGGACCCCA GAAGACGCTT TACCTCCCAG 1861 GTCCCTGGTT GAGCAGCGGA ATCAACCAGG TCATCGTTTT TGAGGAGACG ATGGCGGGCC 1921 CTGCATTACA GTTCACGGAA ACCCCCCACC TGGGCAGGAA CCAGTACATT AAGTGCCCAA 1981 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 2041 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 2101 TATCTTGTGG AAAGGACGAG CCAGAGGAGT AGCTTTAGAC TGAACGCGTT AAGTCgacaa 2161 tcaacctctg gattacaaaa tttgtgaaag att