Construct: ORF TRCN0000468208
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012342.1_s317c1
- Derived from:
- ccsbBroadEn_04241
- DNA Barcode:
- CGTGACCCGGCGCGCTCCCCGACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDRG1 (81572)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468208
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81572 | PDRG1 | p53 and DNA damage regulated 1 | NM_030815.3 | 100% | 100% | |
| 2 | mouse | 68559 | Pdrg1 | p53 and DNA damage regulated 1 | NM_178939.2 | 88.2% | 91.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 489
- ORF length:
- 399
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCTATCACC CGAGGCAGAG CGAGTGCTGC 121 GGTACCTTGT AGAAGTGGAG GAGCTCGCCG AGGAGGTGCT GGCGGACAAG CGGCAGATTG 181 TGGACCTGGA CACTAAAAGG AATCAGAATC GAGAGGGCCT GAGGGCCCTG CAGAAGGATC 241 TCAGCCTCTC TGAAGATGTG ATGGTTTGCT TCGGGAACAT GTTTATCAAG ATGCCTCACC 301 CTGAGACAAA GGAAATGATT GAAAAAGATC AAGATCATCT GGATAAAGAA ATAGAAAAAC 361 TGCGGAAGCA ACTTAAAGTG AAGGTCAACC GCCTTTTTGA GGCCCAAGGC AAACCGGAGC 421 TGAAGGGTTT TAACTTGAAC CCCCTCAACC AGGATGAGCT TAAAGCTCTC AAGGTCATCT 481 TGAAAGGATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 541 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 601 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACGTGAC CCGGCGCGCT CCCCGACTAC 661 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt