Construct: ORF TRCN0000468238
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012259.1_s317c1
- Derived from:
- ccsbBroadEn_02244
- DNA Barcode:
- GCACTTCACATACGTATCAACTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTDSS1 (9791)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468238
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9791 | PTDSS1 | phosphatidylserine synthase 1 | NM_014754.3 | 100% | 100% | |
| 2 | human | 9791 | PTDSS1 | phosphatidylserine synthase 1 | NM_001290225.2 | 69.1% | 69.1% | 0_1ins438 |
| 3 | mouse | 19210 | Ptdss1 | phosphatidylserine synthase 1 | NM_008959.3 | 90.6% | 97.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1485
- ORF length:
- 1419
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc gtcctgcgtg gggagccgga ccctaagcaa ggatgatgtg aactacaaaa 121 tgcatttccg gatgatcaac gagcagcaag tggaggacat caccattgac ttcttctacc 181 ggccgcatac catcaccctg ctcagcttca ccatcgtcag cctcatgtac ttcgccttta 241 ccagggatga ctctgttcca gaagacaaca tctggagagg catcctctct gttattttct 301 tctttcttat catcagtgtg ttagctttcc ccaatggtcc gttcactcga cctcatccag 361 ccttatggcg aatggttttt ggactcagtg tgctctactt cctgttcctg gtattcctac 421 tcttcctgaa tttcgagcag gttaaatctc taatgtattg gctagatcca aatcttcgat 481 acgccacaag ggaagcagat gtcatggagt atgctgtgaa ctgccatgtg atcacctggg 541 agaggattat cagccacttt gatatttttg catttggaca tttctggggc tgggccatga 601 aggccttgct gatccgtagt tacggtctct gctggacaat cagtattacc tgggagctga 661 ctgagctctt cttcatgcat ctcctcccca attttgccga gtgctggtgg gatcaagtca 721 ttctggacat cctgttgtgc aatggcggtg gcatttggct gggcatggtc gtttgccggt 781 ttttagagat gaggacttac cactgggcaa gcttcaagga cattcatacc accaccggga 841 agatcaagag agctgttctg cagttcactc ctgctagctg gacctatgtt cgatggtttg 901 accccaaatc ttcttttcag agagtagctg gagtgtacct tttcatgatc atctggcagc 961 tgactgagtt gaataccttc ttcttgaagc atatctttgt gttccaagcc agtcatccat 1021 taagttgggG TAGAATTCTC TTTATTGGTG GCATCACAGC TCCCACAGTG AGACAGTACT 1081 ACGCTTACCT CACCGACACA CAGTGCAAGC GCGTAGGAAC ACAATGCTGG GTGTTTGGGG 1141 TCATTGGTTT CCTGGAGGCC ATTGTTTGCA TAAAATTTGG ACAAGATCTC TTCTCTAAGA 1201 CCCAAATACT CTATGTTGTG CTTTGGCTTC TTTGCGTGGC TTTCACCACT TTCCTCTGTC 1261 TGTACGGCAT GATTTGGTAT GCAGAACACT ATGGTCACCG AGAAAAGACC TACTCGGAGT 1321 GTGAAGATGG CACCTACAGT CCAGAGATCT CCTGGCATCA CAGGAAAGGG ACAAAAGGTT 1381 CTGAAGACAG CCCACCCAAG CATGCAGGCA ACAACGAAAG CCATTCTTCC AGGAGAAGGA 1441 ATCGGCATTC CAAGTCAAAA GTCACCAATG GCGTTGGAAA GAAATGCCCA ACTTTCTTGT 1501 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1561 AGTAATGAAC TAGTACGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1621 GAAAGGACGA GCACTTCACA TACGTATCAA CTTCACGCGT TAAGTCgaca atcaacctct 1681 ggattacaaa atttgtgaaa gatt