Construct: ORF TRCN0000468252
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001884.1_s317c1
- Derived from:
- ccsbBroadEn_07715
- DNA Barcode:
- CATCGCAGTGGCCGCTCCATTTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMED2 (10959)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468252
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10959 | TMED2 | transmembrane p24 trafficki... | NM_006815.4 | 99.8% | 100% | 30T>C |
| 2 | human | 10959 | TMED2 | transmembrane p24 trafficki... | NM_001321445.2 | 96.4% | 96.6% | 30T>C;373_393del |
| 3 | mouse | 56334 | Tmed2 | transmembrane p24 trafficki... | NM_019770.2 | 91.5% | 99% | (many diffs) |
| 4 | mouse | 100862175 | Gm10698 | predicted gene 10698 | XM_003945446.4 | 91.5% | 99% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 669
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaaa 61 ttggcatggt gacgcttgct gaactgctgg tgctcctggc cgctctcctg gccacggtct 121 cgggctattt cgttagcatc gacgcccatg ctgaagagtg cttctttgag cgggtcacct 181 cgggcaccaa gatgggcctc atcttcgagg tggcggaggg cggcttcctg gacatcgacg 241 tggagattac aggaccagat aacaaaggaa tttacaaagg agacagagaa tccagtggga 301 aatacacatt TGCTGCTCAC ATGGATGGAA CATACAAATT TTGTTTTAGT AACCGGATGT 361 CCACCATGAC TCCAAAAATA GTGATGTTCA CCATTGATAT TGGGGAGGCT CCAAAAGGAC 421 AAGATATGGA AACAGAAGCT CACCAGAACA AGCTAGAAGA AATGATCAAT GAGCTAGCAG 481 TGGCGATGAC AGCTGTAAAG CACGAACAGG AATACATGGA AGTCCGGGAG AGAATACACA 541 GAGCCATCAA CGACAACACA AACAGCAGAG TGGTCCTTTG GTCCTTCTTT GAAGCTCTTG 601 TTCTAGTTGC CATGACATTG GGACAGATCT ACTACCTGAA GAGATTTTTT GAAGTCCGGA 661 GAGTTGTTTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 721 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 781 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACATCGC AGTGGCCGCT CCATTTTCAC 841 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt