Construct: ORF TRCN0000468254
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000530.1_s317c1
- Derived from:
- ccsbBroadEn_11247
- DNA Barcode:
- TCGACGGACATGGGAAAGATCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- REEP5 (7905)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468254
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7905 | REEP5 | receptor accessory protein 5 | NM_005669.5 | 97.8% | 97.8% | 1_12del |
| 2 | human | 7905 | REEP5 | receptor accessory protein 5 | XM_017009844.2 | 70.3% | 65.3% | (many diffs) |
| 3 | mouse | 13476 | Reep5 | receptor accessory protein 5 | NM_007874.3 | 88.7% | 91.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 621
- ORF length:
- 555
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag ggagaggttc gaccggttcc tgcacgagaa gaactgcatg actgaccttc 121 tggccaagct cgaggccaaa accggcgtga acaggagctt catcgctctt ggtgtcatcg 181 gactggtggc cttgtacctg gtgttcggtt atggagcctc tctcctctgc aacctgatag 241 gatttGGCTA CCCAGCCTAC ATCTCAATTA AAGCTATAGA GAGTCCCAAC AAAGAAGATG 301 ATACCCAGTG GCTGACCTAC TGGGTAGTGT ATGGTGTGTT CAGCATTGCT GAATTCTTCT 361 CTGATATCTT CCTGTCATGG TTCCCCTTCT ACTACATGCT GAAGTGTGGC TTCCTGTTGT 421 GGTGCATGGC CCCGAGCCCT TCTAATGGGG CTGAACTGCT CTACAAGCGC ATCATCCGTC 481 CTTTCTTCCT GAAGCACGAG TCCCAGATGG ACAGTGTGGT CAAGGACCTT AAAGACAAGG 541 CCAAAGAGAC TGCAGATGCC ATCACTAAAG AAGCGAAGAA AGCTACCGTG AATTTACTGG 601 GTGAAGAAAA GAAGAGCACC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 661 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 721 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATCGA CGGACATGGG 781 AAAGATCAAC ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt