Construct: ORF TRCN0000468273
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001636.1_s317c1
- Derived from:
- ccsbBroadEn_05878
- DNA Barcode:
- GAGCCCACTTCAGATTCCTACTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BBS4 (585)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468273
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 585 | BBS4 | Bardet-Biedl syndrome 4 | NM_033028.5 | 99.6% | 99.4% | (many diffs) |
| 2 | human | 585 | BBS4 | Bardet-Biedl syndrome 4 | NM_001320665.1 | 95.3% | 94.9% | (many diffs) |
| 3 | human | 585 | BBS4 | Bardet-Biedl syndrome 4 | XM_017022450.1 | 93.3% | 92.2% | (many diffs) |
| 4 | human | 585 | BBS4 | Bardet-Biedl syndrome 4 | NM_001252678.1 | 66.6% | 66.2% | (many diffs) |
| 5 | human | 585 | BBS4 | Bardet-Biedl syndrome 4 | XM_011521848.1 | 66.6% | 66.2% | (many diffs) |
| 6 | human | 585 | BBS4 | Bardet-Biedl syndrome 4 | XM_011521849.1 | 66.6% | 66.2% | (many diffs) |
| 7 | human | 585 | BBS4 | Bardet-Biedl syndrome 4 | XM_017022452.1 | 66.6% | 66.2% | (many diffs) |
| 8 | human | 585 | BBS4 | Bardet-Biedl syndrome 4 | XM_017022453.1 | 66.6% | 66.2% | (many diffs) |
| 9 | human | 585 | BBS4 | Bardet-Biedl syndrome 4 | XM_017022454.1 | 66.6% | 66.2% | (many diffs) |
| 10 | human | 585 | BBS4 | Bardet-Biedl syndrome 4 | NR_045565.1 | 60.3% | (many diffs) | |
| 11 | human | 585 | BBS4 | Bardet-Biedl syndrome 4 | NR_045566.1 | 54.7% | (many diffs) | |
| 12 | human | 585 | BBS4 | Bardet-Biedl syndrome 4 | XM_011521851.1 | 52.7% | 52.4% | (many diffs) |
| 13 | mouse | 102774 | Bbs4 | Bardet-Biedl syndrome 4 (hu... | NM_175325.3 | 88.1% | 89.2% | (many diffs) |
| 14 | mouse | 102774 | Bbs4 | Bardet-Biedl syndrome 4 (hu... | XM_006510755.1 | 78.2% | 78.5% | (many diffs) |
| 15 | mouse | 102774 | Bbs4 | Bardet-Biedl syndrome 4 (hu... | XM_017313095.1 | 59.3% | 59.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1623
- ORF length:
- 1557
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggaggagaga gtcgcgacga gaactcaatt tcctgtatct actgagtctc 121 aaaaaccccg gcagaaaaaa gctccagagt ttcctatttt ggagaagcag aactggttga 181 ttcatcttca ttatatccgg aaagattatg aagcctgcaa ggctgttatc aaagaacagc 241 ttcaagagac tcagggattg tgtgaatatg ctatctatgt ccaagcattg atatttcgcc 301 tagaaggaaa tatccaagaa tccctagaac tcttccagac atgtgcagtt cttagtcctc 361 agagtgctga taacctcaag caggtggcca gatctttatt tcttttggga aaacataaag 421 ctgccattga agtatataat gaagcagcta aactcaacca gaaagattgg gagatcagcc 481 ataacctagg agtttgctac atatacctga agcagttcaa caaggcacaa gaccagttgc 541 acaatgccct gaatcttaat aggcacgatc tgacttatat aatgctgggg aagatccact 601 tgctggaggg agacttggac aaggccattg aagtctacaa gaaagcagtg gagttctcac 661 cagaaaatac agagcttctt acaactttag gattactcta cttacagctc ggcatttacc 721 agaaggcatt tgaacatctt ggcaatgcac tgacttatga ccctaccaac tacaaggcca 781 tcttggcagc aggcagcatg atgcagaccc acggggactt tgatgttgcc ctcaccaaat 841 acagagttgt ggcttgtgct gttccagaaa gtcctccact ctggaataac attggaatgt 901 gtttctttgg caagaagaaa tatgtggcgg ccatcagctg cctgaaacga gccaactact 961 tggcaccctt cgattggaag attctgtata atttgggcct tgtccatttg accatgcagc 1021 agtatgcatc agcttttcat tttctcagtg cggccatcaa cttccagcca aagatggggg 1081 agctctacat gctcttggca gtggctctga ccaatctgga agatacagaa aatgccaaga 1141 gagcctacgc agaagcagtc cacctggata agtgtaaccc tttagtaaac cTGAACTATG 1201 CTGTGCTGCT GTACAACCAG GGCGAGAAGA AGAACGCCCT GGTCCAATAT CAGGAGATGG 1261 AGAAGAAAGT CAGCCTACTC AAGGACAATA GCTCTCTGGA ATTTGACTCT GAGATGGTGG 1321 AGATGGCTCA GAAGTTGGGA GCTGCTCTCC AGGTTGGGGA GGCACTGGTC TGGACCAAAC 1381 CAGTTAAAGA TCCCAAATCA AAGCACCAGA CCACTTCAAC CAGCAAACCT GCCAGTTTCC 1441 AGCAGCCTCT GGGCTCTAAT CAAGCTCTAG GACAGGCAAT GTCTTCAGCA GCTGCATACA 1501 GGACGCTCCC CTCAGGTGCT GGAGGAACAT CCCAGTTCAC AAAGCCCCCA TCTCTTCCTC 1561 TGGAGCCAGA GCCTGCGGTG GAATCAAGTC CAACTGAAAC ATCAGAACAA TTAAGAGAGA 1621 AATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1681 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1741 GGCTTTATAT ATCTTGTGGA AAGGACGAGA GCCCACTTCA GATTCCTACT GTACGCGTTA 1801 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt