Construct: ORF TRCN0000468273

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001636.1_s317c1
Derived from:
ccsbBroadEn_05878
DNA Barcode:
GAGCCCACTTCAGATTCCTACTGT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BBS4 (585)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468273
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 585 BBS4 Bardet-Biedl syndrome 4 NM_033028.5 99.6% 99.4% (many diffs)
2 human 585 BBS4 Bardet-Biedl syndrome 4 NM_001320665.1 95.3% 94.9% (many diffs)
3 human 585 BBS4 Bardet-Biedl syndrome 4 XM_017022450.1 93.3% 92.2% (many diffs)
4 human 585 BBS4 Bardet-Biedl syndrome 4 NM_001252678.1 66.6% 66.2% (many diffs)
5 human 585 BBS4 Bardet-Biedl syndrome 4 XM_011521848.1 66.6% 66.2% (many diffs)
6 human 585 BBS4 Bardet-Biedl syndrome 4 XM_011521849.1 66.6% 66.2% (many diffs)
7 human 585 BBS4 Bardet-Biedl syndrome 4 XM_017022452.1 66.6% 66.2% (many diffs)
8 human 585 BBS4 Bardet-Biedl syndrome 4 XM_017022453.1 66.6% 66.2% (many diffs)
9 human 585 BBS4 Bardet-Biedl syndrome 4 XM_017022454.1 66.6% 66.2% (many diffs)
10 human 585 BBS4 Bardet-Biedl syndrome 4 NR_045565.1 60.3% (many diffs)
11 human 585 BBS4 Bardet-Biedl syndrome 4 NR_045566.1 54.7% (many diffs)
12 human 585 BBS4 Bardet-Biedl syndrome 4 XM_011521851.1 52.7% 52.4% (many diffs)
13 mouse 102774 Bbs4 Bardet-Biedl syndrome 4 (hu... NM_175325.3 88.1% 89.2% (many diffs)
14 mouse 102774 Bbs4 Bardet-Biedl syndrome 4 (hu... XM_006510755.1 78.2% 78.5% (many diffs)
15 mouse 102774 Bbs4 Bardet-Biedl syndrome 4 (hu... XM_017313095.1 59.3% 59.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1623
ORF length:
1557
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc ggaggagaga gtcgcgacga gaactcaatt tcctgtatct actgagtctc
121aaaaaccccg gcagaaaaaa gctccagagt ttcctatttt ggagaagcag aactggttga
181ttcatcttca ttatatccgg aaagattatg aagcctgcaa ggctgttatc aaagaacagc
241ttcaagagac tcagggattg tgtgaatatg ctatctatgt ccaagcattg atatttcgcc
301tagaaggaaa tatccaagaa tccctagaac tcttccagac atgtgcagtt cttagtcctc
361agagtgctga taacctcaag caggtggcca gatctttatt tcttttggga aaacataaag
421ctgccattga agtatataat gaagcagcta aactcaacca gaaagattgg gagatcagcc
481ataacctagg agtttgctac atatacctga agcagttcaa caaggcacaa gaccagttgc
541acaatgccct gaatcttaat aggcacgatc tgacttatat aatgctgggg aagatccact
601tgctggaggg agacttggac aaggccattg aagtctacaa gaaagcagtg gagttctcac
661cagaaaatac agagcttctt acaactttag gattactcta cttacagctc ggcatttacc
721agaaggcatt tgaacatctt ggcaatgcac tgacttatga ccctaccaac tacaaggcca
781tcttggcagc aggcagcatg atgcagaccc acggggactt tgatgttgcc ctcaccaaat
841acagagttgt ggcttgtgct gttccagaaa gtcctccact ctggaataac attggaatgt
901gtttctttgg caagaagaaa tatgtggcgg ccatcagctg cctgaaacga gccaactact
961tggcaccctt cgattggaag attctgtata atttgggcct tgtccatttg accatgcagc
1021agtatgcatc agcttttcat tttctcagtg cggccatcaa cttccagcca aagatggggg
1081agctctacat gctcttggca gtggctctga ccaatctgga agatacagaa aatgccaaga
1141gagcctacgc agaagcagtc cacctggata agtgtaaccc tttagtaaac cTGAACTATG
1201CTGTGCTGCT GTACAACCAG GGCGAGAAGA AGAACGCCCT GGTCCAATAT CAGGAGATGG
1261AGAAGAAAGT CAGCCTACTC AAGGACAATA GCTCTCTGGA ATTTGACTCT GAGATGGTGG
1321AGATGGCTCA GAAGTTGGGA GCTGCTCTCC AGGTTGGGGA GGCACTGGTC TGGACCAAAC
1381CAGTTAAAGA TCCCAAATCA AAGCACCAGA CCACTTCAAC CAGCAAACCT GCCAGTTTCC
1441AGCAGCCTCT GGGCTCTAAT CAAGCTCTAG GACAGGCAAT GTCTTCAGCA GCTGCATACA
1501GGACGCTCCC CTCAGGTGCT GGAGGAACAT CCCAGTTCAC AAAGCCCCCA TCTCTTCCTC
1561TGGAGCCAGA GCCTGCGGTG GAATCAAGTC CAACTGAAAC ATCAGAACAA TTAAGAGAGA
1621AATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1681TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1741GGCTTTATAT ATCTTGTGGA AAGGACGAGA GCCCACTTCA GATTCCTACT GTACGCGTTA
1801AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)