Construct: ORF TRCN0000468284
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002570.1_s317c1
- Derived from:
- ccsbBroadEn_01265
- DNA Barcode:
- GACTCTCTTGCGACAAAGACAAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTPA (5524)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468284
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5524 | PTPA | protein phosphatase 2 phosp... | NM_021131.5 | 100% | 100% | |
| 2 | human | 5524 | PTPA | protein phosphatase 2 phosp... | NM_178000.3 | 100% | 100% | |
| 3 | human | 5524 | PTPA | protein phosphatase 2 phosp... | NM_001271832.1 | 91% | 91% | 127_128ins87 |
| 4 | human | 5524 | PTPA | protein phosphatase 2 phosp... | NM_178001.2 | 90.2% | 90.2% | 214_318del |
| 5 | human | 5524 | PTPA | protein phosphatase 2 phosp... | NM_001193397.1 | 89.1% | 89.1% | 0_1ins105 |
| 6 | human | 5524 | PTPA | protein phosphatase 2 phosp... | XM_017014888.2 | 88.6% | 88.8% | (many diffs) |
| 7 | human | 5524 | PTPA | protein phosphatase 2 phosp... | NM_178003.3 | 86.9% | 86.9% | 213_214ins126 |
| 8 | human | 5524 | PTPA | protein phosphatase 2 phosp... | XM_011518837.2 | 80.6% | 80.6% | (many diffs) |
| 9 | human | 5524 | PTPA | protein phosphatase 2 phosp... | XM_011518838.2 | 78.8% | 78.8% | 1_150del;277_278ins87 |
| 10 | human | 5524 | PTPA | protein phosphatase 2 phosp... | XM_011518835.2 | 70.5% | 67.4% | (many diffs) |
| 11 | human | 5524 | PTPA | protein phosphatase 2 phosp... | XM_011518834.2 | 68.2% | 65.2% | (many diffs) |
| 12 | human | 5524 | PTPA | protein phosphatase 2 phosp... | XM_011518836.2 | 62% | 59% | (many diffs) |
| 13 | mouse | 110854 | Ptpa | protein phosphatase 2 prote... | NM_138748.5 | 89.8% | 96.2% | (many diffs) |
| 14 | mouse | 110854 | Ptpa | protein phosphatase 2 prote... | XM_006497608.2 | 69.8% | 68.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1035
- ORF length:
- 969
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgagggcgag cggcagccgc cgccagattc ttcagaggag gcccctccag 121 ccactcagaa cttcatcatt ccaaaaaagg agatccacac agttccagac atgggcaaat 181 ggaagcgttc tcaggcatac gctgactaca tcggattcat ccttaccctc aacgaaggtg 241 tgaaggggaa gaagctgacc ttcgagtaca gagtctccga ggccattgag aaactagtcg 301 ctcttctcaa cacgctggac aggtggattg atgagactcc tccagtggac cagccctctc 361 ggtttgggaa taaggcatac aggacctggt atgccaaact tgatgaggaa gcagaaaact 421 tggtggccac agtggtccct acccatctgg cagctgctgt gcctgaggtg gctgtttacc 481 taaaggagtc agtggggaac tccacgcgca ttgactacgg cacagggcat gaggcagcct 541 tcgctgcttt cctctgctgt ctctgcaaga ttggggtgct ccgggtggat gaccaaatag 601 ctattgtctt caaggtgttc aatcggtacc ttgaggttat gcggaaactc cagaaaacat 661 acaggatgga gccagccggc agccaggGAG TGTGGGGTCT GGATGACTTC CAGTTTCTGC 721 CCTTCATCTG GGGCAGTTCG CAGCTGATAG ACCACCCATA CCTGGAGCCC AGACACTTTG 781 TGGATGAGAA GGCCGTGAAT GAGAACCACA AGGACTACAT GTTCCTGGAG TGTATCCTGT 841 TTATTACCGA GATGAAGACT GGCCCATTTG CAGAGCACTC CAACCAGCTG TGGAACATCA 901 GCGCCGTCCC TTCCTGGTCC AAAGTGAACC AGGGTCTCAT CCGCATGTAT AAGGCCGAGT 961 GCCTGGAGAA GTTCCCTGTG ATCCAGCACT TCAAGTTCGG GAGCCTGCTG CCCATCCATC 1021 CTGTCACGTC GGGCTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1081 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1141 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA GACTCTCTTG CGACAAAGAC 1201 AAATACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt