Construct: ORF TRCN0000468286
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012548.1_s317c1
- Derived from:
- ccsbBroadEn_06741
- DNA Barcode:
- TGATGCTGGTGCTTGCACTTAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLSCR1 (5359)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468286
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5359 | PLSCR1 | phospholipid scramblase 1 | NM_021105.3 | 99.8% | 99.6% | 947G>T |
| 2 | human | 5359 | PLSCR1 | phospholipid scramblase 1 | XM_011512904.1 | 99.8% | 99.6% | 947G>T |
| 3 | human | 5359 | PLSCR1 | phospholipid scramblase 1 | XM_017006629.1 | 99.8% | 99.6% | 947G>T |
| 4 | human | 5359 | PLSCR1 | phospholipid scramblase 1 | NM_001363872.1 | 97.6% | 97.4% | 0_1ins21;926G>T |
| 5 | human | 5359 | PLSCR1 | phospholipid scramblase 1 | XM_017006628.1 | 94.9% | 91.4% | (many diffs) |
| 6 | human | 5359 | PLSCR1 | phospholipid scramblase 1 | NM_001363874.1 | 74.4% | 66.1% | 0_1ins25;67_68ins218;704G>T |
| 7 | human | 5359 | PLSCR1 | phospholipid scramblase 1 | XM_005247538.3 | 74.4% | 66.1% | 0_1ins25;67_68ins218;704G>T |
| 8 | human | 5359 | PLSCR1 | phospholipid scramblase 1 | XM_011512906.2 | 74.4% | 66.1% | 0_1ins25;67_68ins218;704G>T |
| 9 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | NM_001199978.1 | 73.3% | 66.4% | (many diffs) |
| 10 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_024453670.1 | 73.3% | 66.4% | (many diffs) |
| 11 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_024453671.1 | 73.3% | 66.4% | (many diffs) |
| 12 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_024453672.1 | 73.3% | 66.4% | (many diffs) |
| 13 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_024453673.1 | 73.3% | 66.4% | (many diffs) |
| 14 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_024453674.1 | 73.3% | 66.4% | (many diffs) |
| 15 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | NM_001199979.1 | 72.4% | 62.9% | (many diffs) |
| 16 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_011513022.3 | 71.5% | 61.7% | (many diffs) |
| 17 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_011513023.3 | 71.5% | 61.7% | (many diffs) |
| 18 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_017006903.2 | 71.5% | 61.7% | (many diffs) |
| 19 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_017006904.2 | 71.5% | 61.7% | (many diffs) |
| 20 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | NM_020359.2 | 62.9% | 59.4% | (many diffs) |
| 21 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_017006910.2 | 62.9% | 59.4% | (many diffs) |
| 22 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_017006911.1 | 62.9% | 59.4% | (many diffs) |
| 23 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_024453675.1 | 62.9% | 59.4% | (many diffs) |
| 24 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_024453676.1 | 62.9% | 59.4% | (many diffs) |
| 25 | human | 57047 | PLSCR2 | phospholipid scramblase 2 | XM_024453677.1 | 61.8% | 52.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1020
- ORF length:
- 954
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga caaacaaaac tcacagatga atgcttctca cccggaaaca aacttgccag 121 ttgggtatcc tcctcagtat ccaccgacag cattccaagg acctccagga tatagtggct 181 accctgggcc ccaggtcagc tacccacccc caccagccgg ccattcaggt cctggcccag 241 ctggctttcc tgtcccaaat cagccagtgt ataatcagcc agtatataat cagccagttg 301 gagctgcagg ggtaccatgg atgccagcgc cacagcctcc attaaactgt ccacctggat 361 tagaatattt aagtcagata gatcagatac tgattcatca gcaaattgaa cttctggaag 421 ttttaacagg ttttgaaact aataacaaat atgaaattaa gaacagcttt ggacagaggg 481 tttactttgc agcggaagat actgattgct gtacccgaaa ttgctgtggg ccatctagac 541 cttttacctt gaggattatt gataatatgg gtcaagaagt cataactctg gagagaccac 601 taagatgtag cagctgttgt tgtccctgct gccttcagga gatagaaatc CAAGCTCCTC 661 CTGGTGTACC AATAGGTTAT GTTATTCAGA CTTGGCACCC ATGTCTACCA AAGTTTACAA 721 TTCAAAATGA GAAAAGAGAG GATGTACTAA AAATAAGTGG TCCATGTGTT GTGTGCAGCT 781 GTTGTGGAGA TGTTGATTTT GAGATTAAAT CTCTTGATGA ACAGTGTGTG GTTGGCAAAA 841 TTTCCAAGCA CTGGACTGGA ATTTTGAGAG AGGCATTTAC AGACGCTGAT AACTTTGGAA 901 TCCAGTTCCC TTTAGACCTT GATGTTAAAA TGAAAGCTGT AATGATTGGT GCCTGTTTCC 961 TCATTGACTT CATGTTTTTT GAAAGCACTG GCAGCCAGGA ACAAAAATCA GTAGTGTGGT 1021 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1081 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1141 TTTATATATC TTGTGGAAAG GACGATGATG CTGGTGCTTG CACTTAGCCA CGCGTTAAGT 1201 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt