Construct: ORF TRCN0000468331
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005243.1_s317c1
- Derived from:
- ccsbBroadEn_01048
- DNA Barcode:
- TCTTCATGACGGATTGAACCCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MYO1F (4542)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468331
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4542 | MYO1F | myosin IF | NM_012335.4 | 100% | 100% | |
| 2 | human | 4542 | MYO1F | myosin IF | NM_001348355.2 | 99.6% | 99.6% | 770_771insGAGTGCTATGCA |
| 3 | human | 4542 | MYO1F | myosin IF | XM_011528024.2 | 98.1% | 95.3% | 1797_1845del;1946_1959del |
| 4 | human | 4542 | MYO1F | myosin IF | XM_011528025.2 | 97.7% | 95% | 770_771insGAGTGCTATGCA;1785_1833del;1934_1947del |
| 5 | human | 4542 | MYO1F | myosin IF | XM_011528027.2 | 96.9% | 87.7% | 1524_1525ins86;1811_1824del |
| 6 | human | 4542 | MYO1F | myosin IF | XM_011528026.2 | 95.5% | 92.8% | 1268_1269ins87;1710_1758del;1859_1872del |
| 7 | human | 4542 | MYO1F | myosin IF | XR_001753692.2 | 75.3% | 1_133del;1930_1978del;2802_2803ins674 | |
| 8 | human | 4542 | MYO1F | myosin IF | XR_936180.3 | 66.4% | 1_133del;2030_2043del;2434_2435ins1007 | |
| 9 | human | 4542 | MYO1F | myosin IF | XR_936181.2 | 61.2% | (many diffs) | |
| 10 | human | 4542 | MYO1F | myosin IF | XM_024451522.1 | 61.1% | 57.7% | (many diffs) |
| 11 | human | 4542 | MYO1F | myosin IF | XR_936182.3 | 55% | 1_133del;1734_1735insCAGTGAG;2018_2019ins1402 | |
| 12 | human | 4542 | MYO1F | myosin IF | XM_011528028.3 | 52.1% | 45.6% | (many diffs) |
| 13 | mouse | 17916 | Myo1f | myosin IF | NM_053214.2 | 86.1% | 93.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3360
- ORF length:
- 3294
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg cagcaaggag cgcttccact ggcagagcca caacgtgaag cagagcggcg 121 tggatgacat ggtgcttctt ccccagatca ccgaagacgc cattgccgcc aacctccgga 181 agcgcttcat ggacgactac atcttcacct acatcggctc tgtgctcatc tctgtaaacc 241 ccttcaagca gatgccctac ttcaccgacc gtgagatcga cctctatcag ggcgcggccc 301 agtatgagaa tcccccgcac atctacgccc tcacggacaa catgtaccgg aacatgctta 361 tcgactgtga gaaccagtgt gtcatcatta gtggagagag tggagctggg aagacagtgg 421 cagccaaata tatcatgggc tacatctcca aggtgtctgg cggaggcgag aaggtccagc 481 acgtcaaaga tatcatcctg cagtccaacc cgctgctcga ggccttcggc aacgccaaga 541 ctgtgcgcaa caacaattcc agccgctttg gcaagtactt tgagatccag ttcagccgag 601 gtggggagcc agatgggggc aagatctcca acttcttgct ggagaagtcc cgcgtggtca 661 tgcaaaatga aaatgagagg aacttccaca tctactacca gctgctggaa ggggcctccc 721 aggagcaaag gcagaacctg ggcctcatga caccggacta ctattactac ctcaaccaat 781 cggacaccta ccaggtggac ggcacggacg acagaagcga ctttggtgag actctgagtg 841 ctatgcaggt tattgggatc ccgcccagca tccagcagct ggtcctgcag ctcgtggcgg 901 ggatcttgca cctggggaac atcagtttct gtgaagacgg gaattacgcc cgagtggaga 961 gtgtggacct cctggccttt cccgcctacc tgctgggcat tgacagcggg cgactgcagg 1021 agaagctgac cagccgcaag atggacagcc gctggggcgg gcgcagcgag tccatcaatg 1081 tgaccctcaa cgtggagcag gcagcctaca cccgtgatgc cctggccaag gggctctatg 1141 cccgcctctt cgacttcctc gtggaggcca tcaaccgtgc tatgcagaaa ccccaggaag 1201 agtacagcat cggtgtgctg gacatttacg gcttcgagat cttccagaaa aatggcttcg 1261 agcagttttg catcaacttc gtcaatgaga agctgcagca aatctttatc gaacttaccc 1321 tgaaggccga gcaggaggag tatgtgcagg aaggcatccg ctggactcca atccagtact 1381 tcaacaacaa ggtcgtctgt gacctcatcg aaaacaagct gagcccccca ggcatcatga 1441 gcgtcttgga cgacgtgtgc gccaccatgc acgccacggg cgggggagca gaccagacac 1501 tgctgcagaa gctgcaggcg gctgtgggga cccacgagca tttcaacagc tggagcgccg 1561 gcttcgtcat ccaccactac gctggcaagg tctcctacga cgtcagcggc ttctgcgaga 1621 ggaaccgaga cgttctcttc tccgacctca tagagctgat gcagaccagt gagcaggcct 1681 tcctccggat gctcttcccc gagaagctgg atggagacaa gaaggggcgc cccagcaccg 1741 ccggctccaa gatcaagaaa caagccaacg acctggtggc cacactgatg aggtgcacac 1801 cccactacat ccgctgcatc aaacccaacg agaccaagag gccccgagac tgggaggaga 1861 acagagtcaa gcaccaggtg gaatacctgg gcctgaagga gaacatcagg gtgcgcagag 1921 ccggcttcgc ctaccgccgc cagttcgcca aattcctgca gaggtatgcc attctgaccc 1981 ccgagacgtg gccgcggtgg cgtggggacg aacgccaggg cgtccagcac ctgcttcggg 2041 cggtcaacat ggagcccgac cagtaccaga tggggagcac caaggtcttt gtcaagaacc 2101 cagagtcgct tttcctcctg gaggaggtgc gagagcgaaa gttcgatggc tttgcccgaa 2161 ccatccagaa ggcctggcgg cgccacgtgg ctgtccggaa gtacgaggag atgcgggagg 2221 aagcttccaa catcctgctg aacaagaagg agcggaggcg caacagcatc aatcggaact 2281 tcgtcgggga ctacctgggg ctggaggagc ggcccgagct gcgtcagttc ctgggcaaga 2341 gggagcgggt ggacttcgcc gattcggtca ccaagtacga ccgccgcttc aagcccatca 2401 agcgggactt gatcctgacg cccaagtgtg tgtatgtgat tgggcgagag aaagtgaaga 2461 agggacctga gaagggccag gtgtgtgaag tcttgaagaa gaaagtggac atccaggctc 2521 tgcggggagt ctccctcagc acgcgacagg acgacttctt catcctccaa gaggatgccg 2581 ccgacagctt cctggagagc gtcttcaaga ccgagtttgt cagccttctg tgcaagcgct 2641 tcgaggaggc gacgcggagg cccctgcccc tcaccttcag cgacacacta cagtttcggg 2701 tgaagaagga gggctggggc ggtggcggca cccgcagcgt caccttctcc cgcggcttcg 2761 gcgacttggc agtgctcaag gttggcggtc ggaccctcac ggtcagcgtg ggcgatgggc 2821 tgcccaagag ctccaagcct acgcggaagg gaatggccaa gggaaaacct cggaggtcgt 2881 cccaagcccc tacccgggcg gcccctgcgc cccccagagg catggatcgc aatggggtgc 2941 cccccTCTGC CAGAGGGGGC CCCCTGCCCC TGGAGATCAT GTCTGGAGGG GGCACCCACA 3001 GGCCTCCCCG GGGCCCTCCG TCCACATCCC TGGGAGCCAG CAGACGACCC CGGGCACGTC 3061 CGCCCTCAGA GCACAACACA GAATTCCTCA ACGTGCCTGA CCAGGGCATG GCCGGCATGC 3121 AGAGGAAGCG CAGCGTGGGG CAACGGCCAG TGCCTGGTGT GGGCCGACCC AAGCCCCAGC 3181 CTCGGACACA TGGTCCCAGG TGCCGGGCCC TATACCAGTA CGTGGGCCAA GATGTGGACG 3241 AGCTGAGCTT CAACGTGAAC GAGGTCATTG AGATCCTCAT GGAAGATCCC TCGGGCTGGT 3301 GGAAGGGCCG GCTTCACGGC CAGGAGGGCC TTTTCCCAGG AAACTACGTG GAGAAGATCT 3361 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 3421 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 3481 TTTATATATC TTGTGGAAAG GACGATCTTC ATGACGGATT GAACCCCCTA CGCGTTAAGT 3541 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt