Construct: ORF TRCN0000468341
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001321.1_s317c1
- Derived from:
- ccsbBroadEn_04297
- DNA Barcode:
- CATGCCGTACGCCCACATCGATGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRTAP4-12 (83755)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468341
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83755 | KRTAP4-12 | keratin associated protein ... | NM_031854.3 | 100% | 100% | |
| 2 | human | 100132386 | KRTAP4-9 | keratin associated protein 4-9 | NM_001146041.1 | 89.6% | 86.6% | (many diffs) |
| 3 | human | 81871 | KRTAP4-6 | keratin associated protein 4-6 | NM_030976.1 | 88.8% | 88.5% | (many diffs) |
| 4 | human | 653240 | KRTAP4-11 | keratin associated protein ... | NM_033059.4 | 88.4% | 83.4% | (many diffs) |
| 5 | human | 728224 | KRTAP4-8 | keratin associated protein 4-8 | NM_001322457.1 | 87.6% | 81.9% | (many diffs) |
| 6 | human | 100996750 | LOC100996750 | keratin-associated protein 4-7 | NM_001365252.1 | 86.3% | 83.2% | (many diffs) |
| 7 | human | 85289 | KRTAP4-5 | keratin associated protein 4-5 | NM_033188.4 | 85.2% | 79.6% | (many diffs) |
| 8 | human | 728224 | KRTAP4-8 | keratin associated protein 4-8 | NM_031960.3 | 83.5% | 79% | (many diffs) |
| 9 | human | 100132476 | KRTAP4-7 | keratin associated protein 4-7 | NM_033061.3 | 70% | 68.2% | (many diffs) |
| 10 | human | 84616 | KRTAP4-4 | keratin associated protein 4-4 | NM_032524.2 | 68.3% | 71.6% | (many diffs) |
| 11 | human | 85291 | KRTAP4-2 | keratin associated protein 4-2 | NM_033062.4 | 63.8% | 62.1% | (many diffs) |
| 12 | mouse | 665992 | Krtap4-8 | keratin associated protein 4-8 | NM_001085547.2 | 76.1% | 71.8% | (many diffs) |
| 13 | mouse | 100040248 | Gm11563 | predicted gene 11563 | NM_001126320.2 | 73.9% | 71.6% | (many diffs) |
| 14 | mouse | 670464 | Gm11596 | predicted gene 11596 | NM_001099311.2 | 72.3% | 65.7% | (many diffs) |
| 15 | mouse | 68673 | Krtap4-2 | keratin associated protein 4-2 | NM_026807.2 | 71% | 65.6% | (many diffs) |
| 16 | mouse | 76444 | Krtap4-7 | keratin associated protein 4-7 | NM_029613.1 | 69.5% | 64.5% | (many diffs) |
| 17 | mouse | 665891 | Krtap4-1 | keratin associated protein 4-1 | NM_001048196.1 | 69.5% | 66.3% | (many diffs) |
| 18 | mouse | 665998 | Krtap4-9 | keratin associated protein 4-9 | NM_001085548.2 | 69.3% | 62.4% | (many diffs) |
| 19 | mouse | 546511 | Gm11555 | predicted gene 11555 | XR_106408.1 | 67.8% | (many diffs) | |
| 20 | mouse | 670496 | Gm11564 | predicted gene 11564 | NM_001100614.1 | 65.9% | 65.1% | (many diffs) |
| 21 | mouse | 69464 | Krtap4-13 | keratin associated protein ... | NM_027087.3 | 65% | 64.5% | (many diffs) |
| 22 | mouse | 100040276 | Gm11595 | predicted gene 11595 | NM_001126322.1 | 59.7% | 55.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 669
- ORF length:
- 603
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt caactcctgt tgtggctctg tgtgctctga ccagggctgt ggcctggaga 121 actgctgccg ccccagctgc tgccagacca cctgctgcag gaccacctgc tgccgcccca 181 gctgctgtgt gtccagctgc tgcaggcccc agtgctgcca gtctgtgtgc tgtcagccca 241 cctgctgccg ccccagctgc tgtcagacca cctgctgtag gaccaccTGC TGCCGCCCCA 301 GCTGCTGTGT GTCCAGCTGC TGCAGACCCC AGTGCTGCCA GTCTGTGTGC TGCCAGCCCA 361 CCTGCTGCCG CCCCAGCTGC TGTCAGACCA CCTGCTGCAG GACCACTTGC TGCCGCCCCA 421 GCTGCTGTGT GTCCAGCTGC TGCAGACCCC AGTGCTGCCA GTCTGTGTGC TGCCAGCCCA 481 CCTGCTGCCG CCCCAGCTGC TGCATCTCCA GCAGCTGCTG CCCCTCTTGC TGTGAATCCA 541 GCTGCTGCCG CCCCTGCTGC TGCCTGCGTC CAGTCTGTGG CCGAGTCTCC TGCCACACCA 601 CTTGCTATCG CCCAACCTGT GTCATCTCCA CCTGCCCCCG CCCCTTGTGC TGTGCCTCCT 661 CTTGCTGCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 721 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 781 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACATGCC GTACGCCCAC ATCGATGCAC 841 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt