Construct: ORF TRCN0000468342
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012308.1_s317c1
- Derived from:
- ccsbBroadEn_12955
- DNA Barcode:
- AAGCGGATAATGTGGCTTCTCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM67 (91147)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468342
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 91147 | TMEM67 | transmembrane protein 67 | NM_153704.6 | 98.9% | 98.9% | 1_30del |
| 2 | human | 91147 | TMEM67 | transmembrane protein 67 | XM_006716686.4 | 90.7% | 90.6% | 1_1delAins267;6_7insA;8_9insAAACAT |
| 3 | human | 91147 | TMEM67 | transmembrane protein 67 | NM_001142301.1 | 90.1% | 88.6% | (many diffs) |
| 4 | human | 91147 | TMEM67 | transmembrane protein 67 | NR_024522.2 | 88.9% | 1_51del;3007_3321del | |
| 5 | human | 91147 | TMEM67 | transmembrane protein 67 | XR_428387.2 | 86% | 1_71del;3027_3434del | |
| 6 | human | 91147 | TMEM67 | transmembrane protein 67 | XM_024447326.1 | 78.8% | 78.8% | 0_1ins624 |
| 7 | human | 91147 | TMEM67 | transmembrane protein 67 | XM_011517363.3 | 69.4% | 69.4% | 1_30del;404_405ins882 |
| 8 | human | 91147 | TMEM67 | transmembrane protein 67 | XR_928360.3 | 66.1% | 1_71del;3027_4465del | |
| 9 | human | 91147 | TMEM67 | transmembrane protein 67 | XR_001745619.2 | 66% | 1_71del;3027_4471del | |
| 10 | human | 91147 | TMEM67 | transmembrane protein 67 | XR_928362.3 | 61.2% | 1_71del;2805_2998del;3221_4828del | |
| 11 | mouse | 329795 | Tmem67 | transmembrane protein 67 | NM_177861.4 | 83.9% | 84.4% | (many diffs) |
| 12 | mouse | 329795 | Tmem67 | transmembrane protein 67 | XM_006538007.3 | 78.7% | 79.2% | (many diffs) |
| 13 | mouse | 329795 | Tmem67 | transmembrane protein 67 | XM_006538008.2 | 70.6% | 71.3% | (many diffs) |
| 14 | mouse | 329795 | Tmem67 | transmembrane protein 67 | NR_110955.1 | 69.3% | (many diffs) | |
| 15 | mouse | 329795 | Tmem67 | transmembrane protein 67 | XR_001784156.1 | 57.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3021
- ORF length:
- 2955
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggtttggtcc ctcttatccg cccgggccgt gaccgcgttc cttctgttgt 121 tcctccctcg cttcttacag gcccagacct tctctttccc tttccagcag ccggagaagt 181 gcgacaacaa ccagtacttt gatatctccg ccctctcgtg tgttccttgt ggagctaacc 241 agaggcaaga tgcccgagga acttcatgtg tatgtctacc aggatttcag atgatctcta 301 ataatggagg acctgctatt atttgtaaaa agtgcccaga aaacatgaaa ggtgttacag 361 aagatggctg gaactgcatt tcttgcccta gtgacttaac tgccgaagga aaatgtcact 421 gtcccattgg ccatatttta gtggaaagag acattaatgg aacattgttg tctcaagcaa 481 cttgtgagct ctgtgatgga aatgaaaact cttttatggt agtaaatgct ttaggagaca 541 ggtgcgtccg atgtgagcca acatttgtta ataccagcag gtcctgtgca tgttcagaac 601 ctaacatttt aacaggggga ttatgtttca gcagcacagg gaattttcct ctacgtagaa 661 tttcagctgc acgttatgga gaagttggca tgtctttaac ttcagaatgg tttgcaaagt 721 atttgcaatc atcagcagct gcatgttggg tatatgccaa tctaacatct tgtcaagctc 781 ttggaaatat gtgtgtgatg aacatgaatt cttacgactt tgccacattt gatgcatgtg 841 gactatttca gtttatcttt gaaaatactg ctggactgag cactgttcat tctatttcat 901 tttggagaca gaatcttcct tggctgtttt atggagacca gttaggatta gcacctcaag 961 tgctcagttc tacctctctt cctacaaatt tcagttttaa aggagaaaac cagaatacaa 1021 aactgaagtt tgttgctgct tcctatgata taagaggaaa ttttctcaag tggcaaactt 1081 tagaaggagg tgttttacag ctttgtccag acacagagac aaggctaaat gctgcttatt 1141 catttggaac aacctaccaa caaaattgtg agattcctat ctctaagatc ttaattgact 1201 ttcccactcc tatattttat gatgtgtacc ttgaatatac tgatgaaaat caacatcaat 1261 atattttggc tgtgcctgtg ttaaacctaa atcttcaaca taataagata tttgtgaacc 1321 aagacagcaa ctctggaaag tggcttctaa ctcggcgcat tttcttagtg gatgcagtaa 1381 gtggacgaga aaatgactta ggaactcagc caagagtaat tcgagttgct actcaaatat 1441 cactgagtgt ccaccttgta cccaacacaa taaatggaaa catctaccct cccttaatca 1501 ccattgccta cagtgacatt gatatcaaag atgccaacag ccagtctgtg aaggtttctt 1561 tctcagtcac atatgaaatg gatcatggag aagcacatgt ccagacagat attgctttgg 1621 gtgtattggg tgggctagct gttttagcat ctcttttgaa gacagcagga tggaagaggc 1681 gcattgggag tcccatgatt gatttacaga cagttgtgaa attcttggtg tactatgctg 1741 gtgatctggc caatgttttc tttatcatca cagtgggaac aggtctttac tggcttattt 1801 tcttcaaagc acagaagtct gtgtctgttt tgctgccaat gccaattcag gaagaacgtt 1861 ttgtcactta tgttggatgt gcctttgctc tgaaggcact acaatttttg cataagctca 1921 tatcccagat tacaatagat gtattcttta ttgattggga gcgacctaaa ggaaaggttc 1981 ttaaagctgt tgaaggtgag ggtggtgtac gaagtgccac tgttcctgta agcatatgga 2041 gaacatattt tgtagcaaat gaatggaatg aaattcagac tgtgagaaaa attaattcac 2101 tctttcaagt acttactgtc ctcttctttt tggaggttgt gggattcaag aacttagcat 2161 taatggactc atcttctagt ctttctagaa acccacctag ctacatagct ccttatagct 2221 gcattttgag atatgcagtg tctgctgctc tttggctagc cattggaatt atacaggtcg 2281 tgttctttgc tgtcttttat gagagattta tagaagataa aattcgacag ttcgttgatt 2341 tatgctctat gagtaatata tcagtgtttc tgttatccca caaatgtttt ggatattaca 2401 ttcatggtag atcagtacat gggcatgcag atactaatat ggaagaaatg aatatgaacc 2461 ttaaaagaga agcggaaaat ttgtgtagcc agagaggttt ggtacccaac acagatggtc 2521 agacttttga gattgcaatt tctaaccaga tgagacaaca ttatgacaga attcatgaga 2581 cactaataag gaaaaatggt cctgctagac tactgagttc atcagcaagt acttttgagc 2641 agagtataaa agcatatcat atgatgaata aatttcttgg ctccttcatt gaccatgttc 2701 ataaggaaat ggattacttt ataaaagata agttgcttct tgaaagaatt cttggaatgg 2761 aattcatgga accaatggaa aaaagcatct tttacaatga tgaaggttat tctttcagca 2821 gtgtcctgta ttatggaaat gaagctactc ttcttatttt tgatctgctg ttcttctgtg 2881 ttgtggattt ggcttgccaa aattttattt tagcatcctt ccttacatat cTACAACAAG 2941 AGATTTTTAG ATATATCCGT AATACAGTAG GACAAAAGAA TTTGGCATCC AAAACATTGG 3001 TGGATCAAAG ATTTTTGATT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 3061 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 3121 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAAAGC GGATAATGTG 3181 GCTTCTCGGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt