Construct: ORF TRCN0000468359
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002342.1_s317c1
- Derived from:
- ccsbBroadEn_15778
- DNA Barcode:
- GTTTGGGACACTTAATAAGGCAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PNKD (25953)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468359
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25953 | PNKD | PNKD metallo-beta-lactamase... | NM_001077399.3 | 100% | 100% | |
| 2 | human | 25953 | PNKD | PNKD metallo-beta-lactamase... | XM_017003771.1 | 33.8% | 22.8% | (many diffs) |
| 3 | human | 25953 | PNKD | PNKD metallo-beta-lactamase... | NM_015488.5 | 31.3% | 21.1% | (many diffs) |
| 4 | mouse | 56695 | Pnkd | paroxysmal nonkinesiogenic ... | NM_025580.2 | 90.3% | 90.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 492
- ORF length:
- 426
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcggtggta gctgctacgg cgctgaaggg ccggggggcg agaaatgccc 121 gcgtccTCCG GGGGATTCTC GCAGGAGCCA CAGCTAACAA GGCTTCTCAT AACAGGACCC 181 GGGCCCTGCA AAGCCACAGC TCCCCAGAGG GCAAGGAGGA ACCTGAACCC CTATCCCCGG 241 AGCTGGAATA CATTCCCAGA AAGAGGGGCA AGAACCCCAT GAAAGCTGTG GGACTGGCCT 301 GGGCCATCGG CTTCCCTTGT GGTATCCTCC TCTTCATCCT CACCAAGCGG GAAGTGGACA 361 AGGACCGTGT GAAGCAGATG AAGGCTCGGC AGAACATGCG GTTGTCCAAC ACGGGCGAGT 421 ATGAGAGCCA GAGGTTCAGG GCTTCCTCCC AGAGTGCCCC GTCCCCTGAT GTTGGGTCTG 481 GGGTGCAGAC CTGCCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 541 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 601 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTT TGGGACACTT AATAAGGCAG 661 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t