Construct: ORF TRCN0000468369
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004425.1_s317c1
- Derived from:
- ccsbBroadEn_14022
- DNA Barcode:
- TGGCCCGAAACGGGTGGCTGAATA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GOSR1 (9527)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468369
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9527 | GOSR1 | golgi SNAP receptor complex... | XM_017025375.2 | 62% | 44.8% | (many diffs) |
| 2 | human | 9527 | GOSR1 | golgi SNAP receptor complex... | XM_017025376.2 | 61.2% | 44.1% | (many diffs) |
| 3 | human | 9527 | GOSR1 | golgi SNAP receptor complex... | NM_004871.3 | 59.9% | 43.2% | (many diffs) |
| 4 | human | 9527 | GOSR1 | golgi SNAP receptor complex... | NM_001007025.2 | 59.1% | 42.5% | (many diffs) |
| 5 | human | 9527 | GOSR1 | golgi SNAP receptor complex... | XM_017025377.1 | 50.3% | 49.3% | (many diffs) |
| 6 | human | 9527 | GOSR1 | golgi SNAP receptor complex... | XM_017025374.2 | 50.2% | 35.7% | (many diffs) |
| 7 | human | 9527 | GOSR1 | golgi SNAP receptor complex... | XM_005258070.5 | 48.8% | 34.6% | (many diffs) |
| 8 | human | 9527 | GOSR1 | golgi SNAP receptor complex... | XM_005258071.5 | 48.2% | 34% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 591
- ORF length:
- 525
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcagggacc agcagttact gggaagatct caggaaacag gctcgacagc 121 tggaaaatga acttgacctg aaactagttt ccttcagcaa actatgtaca agttacagtc 181 atagcagtac ccgagatgga agacgcgaca ggtatagttc TGATACAACA CCCCTTTTAA 241 ATGGATCAAG CCAAGACAGA ATGTTTGAGA CAATGGCGAT TGAGATTGAA CAACTTTTGG 301 CAAGGCTTAC AGGGGTAAAT GATAAAATGG CAGAATATAC CAACAGTGCA GGTGTCCCCT 361 CCTTGAATGC AGCCCTGATG CATACATTAC AGCGGCATAG AGACATATTG CAGGTAATAT 421 ATTGGGCTCG AGATGTTTTC ATTATCACAG GAGTTTGGGT TTTTTTTTTT AATCCCTGCA 481 TTGGATATGT GCACATATAT TTAAAAGGAC AAAGAGAAAA ATCTGAGAAA ATAAATGCCA 541 TGCTAAAGGG ACTTGTTTTA CTTTTTTTTG GGGTCACCCT TATAAAATTC TGCCCAACTT 601 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 661 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 721 CTTGTGGAAA GGACGATGGC CCGAAACGGG TGGCTGAATA ACGCGTTAAG TCgacaatca 781 acctctggat tacaaaattt gtgaaagatt