Construct: ORF TRCN0000468429
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015949.1_s317c1
- Derived from:
- ccsbBroadEn_07781
- DNA Barcode:
- TCTCCGTCTACCTCTTCTCTCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HPS5 (11234)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468429
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | NM_007216.4 | 99.9% | 99.9% | 480C>A;2704G>A |
| 2 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | NM_181508.1 | 99.9% | 99.9% | 480C>A;2704G>A |
| 3 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XM_017017154.1 | 99.9% | 99.9% | 480C>A;2704G>A |
| 4 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XM_011519866.1 | 96.8% | 95% | (many diffs) |
| 5 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XM_011519867.1 | 96.8% | 95% | (many diffs) |
| 6 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XM_011519868.3 | 96.8% | 95% | (many diffs) |
| 7 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XM_017017152.1 | 92.8% | 92.8% | 1_231del;711C>A;2935G>A |
| 8 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XM_017017153.2 | 92.8% | 92.8% | 1_231del;711C>A;2935G>A |
| 9 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XM_011519865.1 | 90.1% | 88.5% | (many diffs) |
| 10 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XM_017017151.2 | 90.1% | 88.5% | (many diffs) |
| 11 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | NM_181507.2 | 89.8% | 89.8% | 1_342del;822C>A;3046G>A |
| 12 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XM_017017149.1 | 89.8% | 89.8% | 1_342del;822C>A;3046G>A |
| 13 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XM_017017150.1 | 89.8% | 89.8% | 1_342del;822C>A;3046G>A |
| 14 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XM_011519862.1 | 87.2% | 85.7% | (many diffs) |
| 15 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XM_011519863.1 | 87.2% | 85.7% | (many diffs) |
| 16 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XM_011519864.1 | 87.2% | 85.7% | (many diffs) |
| 17 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XR_001747753.1 | 61.9% | (many diffs) | |
| 18 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XR_001747751.1 | 61.7% | (many diffs) | |
| 19 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XR_001747752.1 | 61.7% | (many diffs) | |
| 20 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XR_001747750.1 | 60.3% | (many diffs) | |
| 21 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XR_001747754.2 | 50.9% | (many diffs) | |
| 22 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XR_001747755.2 | 49.3% | (many diffs) | |
| 23 | human | 11234 | HPS5 | HPS5 biogenesis of lysosoma... | XR_001747756.2 | 48.5% | (many diffs) | |
| 24 | mouse | 246694 | Hps5 | HPS5, biogenesis of lysosom... | XM_006540907.3 | 36.1% | 35.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3111
- ORF length:
- 3045
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta tgtgtcttca gaacacaaag gccgaagagt cacagctctc tgctgggata 121 cagctattct tagagttttt gtaggtgatc atgctgggaa ggtttctgct atcaaactca 181 atacttctaa acaagcaaag gcagctgctg cttttgtgat gtttcctgtt cagacaatca 241 caactgttga ctcctgtgtt gtacagttag attatttgga tggaaggcta cttatatctt 301 cacttactcg atccttcttg tgtgacactg agagagaaaa gttttggaaa attggaaaca 361 aggaaagaga tggagaatat ggagcttgtt tctttcctgg aagatgttct gggggccagc 421 aacctctgat atattgtgct cgcccaggct ctaggatgtg ggaagtgaac tttgatggag 481 aagttataag tacacatcag ttcaagaaac tcctctcgtt gccacctctc cctgtgatta 541 ctctaagatc agaacctcag tatgatcata cagctggatc ctcccagtct ttgtctttcc 601 ccaaactctt acatcttagt gagcattgtg tgctgacttg gacagaaaga ggaatttata 661 ttttcattcc tcagaatgtt caagttcttc tttggagtga agtcaaagat attcaggatg 721 tggctgtctg taggaatgaa ttgttctgtt tgcacctaaa tgggaaagtc tcacatctct 781 ccctgatatc tgtggagcgc tgtgtggaac gcctgctaag aagaggccta tggaacttgg 841 ctgctcgtac atgctgtctt ttccaaaatt ctgtcattgc cagcagagca agaaaaactt 901 tgactgcaga taaattggag catttgaaat ctcagctgga ccatggcacc tacaatgatc 961 taatttctca actggaagaa ttgatcttaa aatttgaacc tttggattca gcttgtagca 1021 gtagaagaag ctccatttca tcacatgaaa gtttcagcat cttggactct ggtatttatc 1081 gtatcattag tagtagaaga ggcagtcagt cagatgaaga ctcttgctcc cttcacagcc 1141 aaaccctctc agaagatgag agatttaaag aattcacctc acagcaggaa gaggacctgc 1201 cagatcagtg ttgtggctca cacggaaatg aagacaatgt ttctcatgct ccagtgatgt 1261 ttgagacaga taagaatgaa acttttctcc cgttcggcat tccattacca tttcgttctc 1321 catctcctct tgtgtctctt caggctgtca aagaaagtgt ttctagcttt gtgcgtaaaa 1381 ctactgagaa gattggcacc cttcacacga gccctgatct gaaagtgaga ccagagctca 1441 ggggtgatga gcaatcatgt gaagaggatg tgagttcaga tacctgccca aaggaggaag 1501 acactgagga ggaaaaagag gtaactagtc cacctccaga agaagacagg ttccaggagc 1561 ttaaagtagc aacagcagaa gcaatgacca agctacagga ccctctggtt ttatttgaat 1621 ccgagtctct gagaatggtt ttacaggagt ggctttcaca tttagaaaaa acatttgcca 1681 tgaaggactt ttcaggtgtt tcagatactg acaactcatc catgaaattg aaccaggatg 1741 tgctattagt taatgaatca aaaaagggaa tattagatga agataatgaa aaagaaaaaa 1801 gggactcttt aggcaatgaa gaatctgttg ataaaacagc atgtgaatgt gtaaggagtc 1861 caagggagtc tttggatgac ctgtttcaaa tatgttctcc atgcgccatt gcaagtggtc 1921 ttcggaacga cctggctgaa ttgacaacat tatgtttgga gttgaatgta ttgaattcta 1981 agatcaaaag caccagtgga catgtggacc acactttgca acagtactct cctgaaattc 2041 tggcttgcca gttcctgaag aagtactttt ttctcctgaa cttgaaaaga gcgaaggaga 2101 gtatcaagct tagttacagt aatagccctt ctgtttggga tacttttatt gaaggattga 2161 aagaaatggc aagttccaat cctgtgtata tggagatgga aaaaggagat ctaccaacaa 2221 ggttaaagtt actagatgac gaggttcctt ttgatagtcc gttgttggtt gtttatgcta 2281 cccggttgta tgaaaagttt ggggagtctg ctcttcgatc cttaatcaag ttctttccat 2341 ccattttgcc atcggatatc atacaacttt gtcatcatca tcctgctgag tttttggcct 2401 atttagacag tctggtgaaa tcaaggcctg aagatcagcg gtcatctttt cttgagtccc 2461 ttctgcaacc agagtcttta aggttggatt ggctgctttt ggcagtgtcc cttgatgctc 2521 caccaagcac cagcacaatg gatgatgaag gttatcccag gcctcattca cacttgcttt 2581 cctggggtta cagtcagctg atccttcatc taattaaact tcctgcagat tttataacca 2641 aagagaaaat gacagacatc tgcaggtctt gtggtttctg gcctggatat ctaattctct 2701 gtttGGAGCT GGAGAGAAGA AGAGAGGCCT TCACCAATAT TGTGTATCTG AATGATATGA 2761 GCCTGATGAA AGGGGACAAT GGTTGGATCC CAGAGACCGT GGAGGAATGG AAGCTTCTCC 2821 TTCATCTCAT ACAGAGCAAG AGCACGAGGC CAGCCCCCCA GGAGTCACTA AATGGGAGCC 2881 TCAGTGATGG GCCTTCCCCC ATCAATGTGG AGAATGTGGC ACTTCTGTTA GCTAAGGCCA 2941 TGGGCCCAGA TCGGGCTTGG TCACTGCTAC AGGAATGTGG TCTGGCCCTT GAGTTGTCAG 3001 AGAAGTTTAC CAGAACCTGC GATATCCTGA GGATTGCTGA GAAAAGGCAG AGGGCCTTGA 3061 TACAAAGCAT GCTTGAAAAA TGCGATCGGT TTCTCTGGTC CCAGCAGGCC TACCCAACTT 3121 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 3181 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 3241 CTTGTGGAAA GGACGATCTC CGTCTACCTC TTCTCTCCCC ACGCGTTAAG TCgacaatca 3301 acctctggat tacaaaattt gtgaaagatt