Construct: ORF TRCN0000468440
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001413.1_s317c1
- Derived from:
- ccsbBroadEn_15512
- DNA Barcode:
- CTTAAATGGTTTCAAGAATTCAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPM1 (4869)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468440
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4869 | NPM1 | nucleophosmin 1 | NM_001355007.1 | 93% | 93% | 1_48del |
| 2 | human | 4869 | NPM1 | nucleophosmin 1 | NM_001355006.1 | 72.7% | 72.7% | 1_240del |
| 3 | human | 4869 | NPM1 | nucleophosmin 1 | NM_002520.6 | 72.7% | 72.7% | 1_240del |
| 4 | human | 4869 | NPM1 | nucleophosmin 1 | NM_001355010.1 | 72.4% | 69% | (many diffs) |
| 5 | human | 4869 | NPM1 | nucleophosmin 1 | NM_199185.3 | 62.9% | 62.9% | 1_240del;579_580ins87 |
| 6 | human | 4869 | NPM1 | nucleophosmin 1 | NM_001037738.3 | 60.6% | 60.2% | (many diffs) |
| 7 | human | 4869 | NPM1 | nucleophosmin 1 | NM_001355009.2 | 50.7% | 50.3% | (many diffs) |
| 8 | human | 4869 | NPM1 | nucleophosmin 1 | NR_149149.1 | 35.4% | 1_485del;641_642ins128;1000_1321del | |
| 9 | human | 29121 | CLEC2D | C-type lectin domain family... | NR_036693.3 | 11.1% | (many diffs) | |
| 10 | mouse | 18148 | Npm1 | nucleophosmin 1 | NM_001252260.1 | 68.9% | 70.9% | (many diffs) |
| 11 | mouse | 18148 | Npm1 | nucleophosmin 1 | NM_008722.3 | 65.8% | 67.7% | (many diffs) |
| 12 | mouse | 18148 | Npm1 | nucleophosmin 1 | NM_001252261.1 | 54.4% | 55.2% | (many diffs) |
| 13 | mouse | 102639044 | LOC102639044 | nucleophosmin pseudogene | XR_389514.3 | 40% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 708
- ORF length:
- 642
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgtacagcca acggtttccc ttgggggctt tgaaataaca ccaccagtgg 121 tcttaaggtt gaagtgtggt tcagggccag tgcatattag tggacagcac ttagtagctg 181 tggaggaaga tgcagagtca gaagatgaag aggaggagga tgtgaaactc ttaagtatat 241 ctggaaagcg gTCTGCCCCT GGAGGTGGTA GCAAGGTTCC ACAGAAAAAA GTAAAACTTG 301 CTGCTGATGA AGATGATGAC GATGATGATG AAGAGGATGA TGATGAAGAT GATGATGATG 361 ATGATTTTGA TGATGAGGAA GCTGAAGAAA AAGCGCCAGT GAAGAAATCT ATACGAGATA 421 CTCCAGCCAA AAATGCACAA AAGTCAAATC AGAATGGAAA AGACTCAAAA CCATCATCAA 481 CACCAAGATC AAAAGGACAA GAATCCTTCA AGAAACAGGA AAAAACTCCT AAAACACCAA 541 AAGGACCTAG TTCTGTAGAA GACATTAAAG CAAAAATGCA AGCAAGTATA GAAAAAGGTG 601 GTTCTCTTCC CAAAGTGGAA GCCAAATTCA TCAATTATGT GAAGAATTGC TTCCGGATGA 661 CTGACCAAGA GGCTATTCAA GATCTCTGGC AGTGGAGGAA GTCTCTTTAC CCAACTTTCT 721 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 781 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 841 GTGGAAAGGA CGACTTAAAT GGTTTCAAGA ATTCAACACG CGTTAAGTCg acaatcaacc 901 tctggattac aaaatttgtg aaagatt