Transcript: Human NM_001355010.1

Homo sapiens nucleophosmin 1 (NPM1), transcript variant 6, mRNA.

Source:
NCBI, updated 2019-09-15
Taxon:
Homo sapiens (human)
Gene:
NPM1 (4869)
Length:
1068
CDS:
246..749

Additional Resources:

NCBI RefSeq record:
NM_001355010.1
NBCI Gene record:
NPM1 (4869)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001355010.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000293952 GAACTTTCCCTACCGTGTTTG pLKO_005 844 3UTR 100% 10.800 6.480 N NPM1 n/a
2 TRCN0000062272 CCTAGTTCTGTAGAAGACATT pLKO.1 585 CDS 100% 4.950 2.475 Y NPM1 n/a
3 TRCN0000286484 CCTAGTTCTGTAGAAGACATT pLKO_005 585 CDS 100% 4.950 2.475 Y NPM1 n/a
4 TRCN0000062268 GCCAAGAATGTGTTGTCCAAA pLKO.1 888 3UTR 100% 4.950 2.475 Y NPM1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001355010.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15512 pDONR223 0% 72.4% 69% None (many diffs) n/a
2 ccsbBroad304_15512 pLX_304 0% 72.4% 69% V5 (many diffs) n/a
3 TRCN0000468440 CTTAAATGGTTTCAAGAATTCAAC pLX_317 70.5% 72.4% 69% V5 (many diffs) n/a
4 ccsbBroadEn_01107 pDONR223 100% 63% 63% None 57_58ins294 n/a
5 ccsbBroad304_01107 pLX_304 0% 63% 63% V5 57_58ins294 n/a
6 TRCN0000471859 CTTGTCTTCTCCATATCTTTCTCA pLX_317 46.6% 63% 63% V5 57_58ins294 n/a
7 ccsbBroadEn_15510 pDONR223 0% 56.8% 56.8% None 57_58ins294;285_286ins87 n/a
8 ccsbBroad304_15510 pLX_304 0% 56.8% 56.8% V5 57_58ins294;285_286ins87 n/a
9 ccsbBroadEn_06654 pDONR223 100% 56.6% 56.8% None 57_58ins294;162A>G;285_286ins87 n/a
10 ccsbBroad304_06654 pLX_304 0% 56.6% 56.8% V5 57_58ins294;162A>G;285_286ins87 n/a
11 TRCN0000481449 CGGGGTCGACTAGCAAACTTCTCG pLX_317 50.7% 56.6% 56.8% V5 57_58ins294;162A>G;285_286ins87 n/a
12 ccsbBroadEn_06655 pDONR223 100% 56.6% 56.4% None 57_58ins294;285_286ins87;478C>T n/a
13 ccsbBroad304_06655 pLX_304 0% 56.6% 56.4% V5 (not translated due to frame shift) 57_58ins294;285_286ins87;478C>T n/a
14 TRCN0000469435 GATTGGGAGCTCAAATAACAAACG pLX_317 51.8% 56.6% 56.4% V5 (not translated due to prior stop codon) 57_58ins294;285_286ins87;478C>T n/a
15 ccsbBroadEn_15511 pDONR223 0% 56.5% 56.1% None (many diffs) n/a
16 ccsbBroad304_15511 pLX_304 0% 56.5% 56.1% V5 (many diffs) n/a
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