Construct: ORF TRCN0000468451
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013541.1_s317c1
- Derived from:
- ccsbBroadEn_00566
- DNA Barcode:
- GGAAATTAGAAAAGTCTCATCAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FHIT (2272)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468451
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2272 | FHIT | fragile histidine triad dia... | NM_001166243.3 | 100% | 100% | |
| 2 | human | 2272 | FHIT | fragile histidine triad dia... | NM_001320899.2 | 100% | 100% | |
| 3 | human | 2272 | FHIT | fragile histidine triad dia... | NM_001320900.2 | 100% | 100% | |
| 4 | human | 2272 | FHIT | fragile histidine triad dia... | NM_002012.4 | 100% | 100% | |
| 5 | human | 2272 | FHIT | fragile histidine triad dia... | NM_001354589.2 | 84.3% | 84.3% | 277_278ins69 |
| 6 | human | 2272 | FHIT | fragile histidine triad dia... | NM_001354590.2 | 84.3% | 84.3% | 277_278ins69 |
| 7 | human | 2272 | FHIT | fragile histidine triad dia... | NM_001320901.2 | 61.4% | 59.1% | (many diffs) |
| 8 | human | 2272 | FHIT | fragile histidine triad dia... | NR_135491.2 | 6.9% | (many diffs) | |
| 9 | mouse | 14198 | Fhit | fragile histidine triad gene | NM_001308286.1 | 85.5% | 89.3% | (many diffs) |
| 10 | mouse | 14198 | Fhit | fragile histidine triad gene | NM_010210.3 | 85.5% | 89.3% | (many diffs) |
| 11 | mouse | 14198 | Fhit | fragile histidine triad gene | XM_006517927.3 | 85.5% | 89.3% | (many diffs) |
| 12 | mouse | 14198 | Fhit | fragile histidine triad gene | XM_011244732.2 | 85.5% | 89.3% | (many diffs) |
| 13 | mouse | 14198 | Fhit | fragile histidine triad gene | XM_017315847.1 | 85.5% | 89.3% | (many diffs) |
| 14 | mouse | 14198 | Fhit | fragile histidine triad gene | XM_006517924.1 | 69.3% | 72.4% | (many diffs) |
| 15 | mouse | 14198 | Fhit | fragile histidine triad gene | NM_001308285.1 | 60.2% | 62.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc gttcagattt ggccaacatc tcatcaagcc ctctgtagtg tttctcaaaa 121 cagaactgtc cTTCGCTCTT GTGAATAGGA AACCTGTGGT ACCAGGACAT GTCCTTGTGT 181 GCCCGCTGCG GCCAGTGGAG CGCTTCCATG ACCTGCGTCC TGATGAAGTG GCCGATTTGT 241 TTCAGACGAC CCAGAGAGTC GGGACAGTGG TGGAAAAACA TTTCCATGGG ACCTCTCTCA 301 CCTTTTCCAT GCAGGATGGC CCCGAAGCCG GACAGACTGT GAAGCACGTT CACGTCCATG 361 TTCTTCCCAG GAAGGCTGGA GACTTTCACA GGAATGACAG CATCTATGAG GAGCTCCAGA 421 AACATGACAA GGAGGACTTT CCTGCCTCTT GGAGATCAGA GGAGGAAATG GCAGCAGAAG 481 CCGCAGCTCT GCGGGTCTAC TTTCAGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGGAAATTA 661 GAAAAGTCTC ATCAGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 721 aagatt