Construct: ORF TRCN0000468458

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018864.3_s317c1
Derived from:
ccsbBroadEn_14583
DNA Barcode:
GCTCCATCGTAGTTTCAGGTTTAT
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
CHKA (1119)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468458
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1119 CHKA choline kinase alpha XM_017017148.2 77.4% 6.9% (many diffs)
2 human 1119 CHKA choline kinase alpha NM_001277.3 76.9% 20.9% (many diffs)
3 human 1119 CHKA choline kinase alpha NM_212469.1 73% 17% (many diffs)
4 human 1119 CHKA choline kinase alpha XM_017017147.1 68.8% 18.5% (many diffs)
5 human 1119 CHKA choline kinase alpha XR_428904.3 53.2% (many diffs)
6 human 1119 CHKA choline kinase alpha XR_949772.2 34.3% (many diffs)
7 human 1119 CHKA choline kinase alpha XR_949773.2 32.1% (many diffs)
8 human 1119 CHKA choline kinase alpha XR_428905.3 11.8% (many diffs)
9 mouse 12660 Chka choline kinase alpha NM_001271496.1 68% 20.9% (many diffs)
10 mouse 12660 Chka choline kinase alpha XM_006531648.2 67% 6.6% (many diffs)
11 mouse 12660 Chka choline kinase alpha XM_006531649.2 67% 6.6% (many diffs)
12 mouse 12660 Chka choline kinase alpha XM_006531650.2 67% 6.6% (many diffs)
13 mouse 12660 Chka choline kinase alpha XM_006531651.3 67% 6.6% (many diffs)
14 mouse 12660 Chka choline kinase alpha XM_011248599.2 67% 6.6% (many diffs)
15 mouse 12660 Chka choline kinase alpha XM_011248600.2 67% 6.6% (many diffs)
16 mouse 12660 Chka choline kinase alpha XM_017318048.1 67% 6.6% (many diffs)
17 mouse 12660 Chka choline kinase alpha XM_017318049.1 67% 6.6% (many diffs)
18 mouse 12660 Chka choline kinase alpha XM_017318050.1 67% 6.6% (many diffs)
19 mouse 12660 Chka choline kinase alpha XM_006531646.1 66.5% 20.4% (many diffs)
20 mouse 12660 Chka choline kinase alpha NM_013490.4 64.1% 17.1% (many diffs)
21 mouse 12660 Chka choline kinase alpha XR_388248.2 40.4% (many diffs)
22 mouse 12660 Chka choline kinase alpha NR_073190.1 34.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
417
ORF length:
348
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaaaaccaaa ttctgcaccg ggggcgagga cgagttccac atcagtgtca
121tcagaggcgg ccttagcaac atgctgttcc agtgctccct acctgacacc acagccaccc
181ttggtgatga gcctcggaaa gtgctcctgc ggctgtatgg agcgattttg cagatgaggt
241cctgtaataa agagggatcc gaacaagctc agaaagaaaa tgaatttcaa ggggctgagg
301ccatggttct ggagagcgtt atgtttgcca ttctcgcaga gaggtcactt ggnccaaaac
361tntatgggca tctttcccca agggccgact ggagcagttc atcccgagcc ggcgattaga
421tactgannaa ttaagtttgc cagatatttc tgcagaaatc gccgagaaaa tggctacatt
481tcatggtatg aaaatgccat tcaataaggn accaaaatgg ctttttggca caatggaaaa
541gtatctaaag gaagtgctga gaattaaatt tactgaggaa tccagaatta aaaagctcca
601caaattgctc agttacaatc tgcccttgga actggaaaac ctgagatcat tgcttgaatc
661tactccatct ccagttgtat tttgtcataa tgactgtcaa gaaggtaata tcttgttgct
721ggaangccga gagaattctg aaaaacagaa actgatgctc attgatttcg aatacagcag
781ttacanttac agggGATTCG ACATTGGAAA TCACTTCTGT GAGTGGATGT ATGATTATAG
841CTATGAAAAA TACCCTTTTT TCAGAGCAAA CATCCGGAAG TATCCCACCA AGAAACAACA
901GCTCCATTTT ATTTCCAGTT ACTTGCCTGC ATTCCAAAAT GACTTTGAAA ACCTCAGTAC
961TGAAGAAAAA TCCATTATAA AAGAAGAAAT GTTGCTTGAA GTTAATAGGT TTGCCCTTGC
1021ATCTCATTTC CTCTGGGGAC TGTGGTCCAT TGTACAAGCC AAGATTTCAT CTATTGAATT
1081TGGGTACATG GACTACGCCC AAGCAAGGTT TGATGCCTAT TTCCACCAGA AGAGGAAGCT
1141TGGGGTGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC
1201TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT
1261TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAGCTCCAT CGTAGTTTCA GGTTTATACG
1321CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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