Construct: ORF TRCN0000468494
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011266.1_s317c1
- Derived from:
- ccsbBroadEn_03441
- DNA Barcode:
- AAATCATCCCCTACTGAATCTAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UGT1A9 (54600)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468494
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54600 | UGT1A9 | UDP glucuronosyltransferase... | NM_021027.3 | 100% | 100% | |
| 2 | human | 54576 | UGT1A8 | UDP glucuronosyltransferase... | NM_019076.4 | 96.4% | 94.9% | (many diffs) |
| 3 | human | 54577 | UGT1A7 | UDP glucuronosyltransferase... | NM_019077.2 | 95.8% | 93.3% | (many diffs) |
| 4 | human | 54575 | UGT1A10 | UDP glucuronosyltransferase... | NM_019075.2 | 95.7% | 93.2% | (many diffs) |
| 5 | human | 54578 | UGT1A6 | UDP glucuronosyltransferase... | NM_205862.2 | 49.2% | 48.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1656
- ORF length:
- 1590
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttgcacaggg tggaccagcc cccttcctct atgtgtgtgt ctgctgctga 121 cctgtggctt tgccgaggca gggaagctac tggtagtgcc catggatggg agccactggt 181 tcaccatgag gtcggtggtg gagaaactca ttctcagggg gcatgaggtg gttgtagtca 241 tgccagaggt gagttggcaa ctgggaagat cactgaattg cacagtgaag acttattcaa 301 cttcatatac cctggaggat ctggaccggg agttcaaggc ttttgcccat gctcaatgga 361 aagcacaagt acgaagtata tattctctat taatgggttc atacaatgac atttttgact 421 tatttttttc aaattgcagg agtttgttta aagacaaaaa attagtagaa tacttaaagg 481 agagttcttt tgatgcagtg tttctcgatc cttttgataa ctgtggctta attgttgcca 541 aatatttctc cctcccctcc gtggtcttcg ccaggggaat actttgccac tatcttgaag 601 aaggtgcaca gtgccctgct cctctttcct atgtccccag aattctctta gggttctcag 661 atgccatgac tttcaaggag agagtacgga accacatcat gcacttggag gaacatttat 721 tatgccaccg ttttttcaaa aatgccctag aaatagcctc tgaaattctc caaacacctg 781 ttacggagta tgatctctac agccacacat caatttggtt gttgcgaacg gactttgttt 841 tggactatcc caaacccgtg atgcccaaca tgatcttcat tggtggtatc aactgccatc 901 agggaaagcc gttgcctatg gaatttgaag cctacattaa tgcttctgga gaacatggaa 961 ttgtggtttt ctctttggga tcaatggtct cagaaattcc agagaagaaa gctatggcaa 1021 ttgctgatgc tttgggcaaa atccctcaga cagtcctgtg gcggtacact ggaacccgac 1081 catcgaatct tgcgaacaac acgatacttg ttaagtggct accccaaaac gatctgcttg 1141 gtcacccgat gacccgtgcc tttatcaccc atgctggttc ccatggtgtt tatgaaagca 1201 tatgcaatgg cgttccCATG GTGATGATGC CCTTGTTTGG TGATCAGATG GACAATGCAA 1261 AGCGCATGGA GACTAAGGGA GCTGGAGTGA CCCTGAATGT TCTGGAAATG ACTTCTGAAG 1321 ATTTAGAAAA TGCTCTAAAA GCAGTCATCA ATGACAAAAG TTACAAGGAG AACATCATGC 1381 GCCTCTCCAG CCTTCACAAG GACCGCCCGG TGGAGCCGCT GGACCTGGCC GTGTTCTGGG 1441 TGGAGTTTGT GATGAGGCAC AAGGGCGCGC CACACCTGCG CCCCGCAGCC CACGACCTCA 1501 CCTGGTACCA GTACCATTCC TTGGACGTGA TTGGTTTCCT CTTGGCCGTC GTGCTGACAG 1561 TGGCCTTCAT CACCTTTAAA TGTTGTGCTT ATGGCTACCG GAAATGCTTG GGGAAAAAAG 1621 GGCGAGTTAA GAAAGCCCAC AAATCCAAGA CCCATTGCCC AACTTTCTTG TACAAAGTGG 1681 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1741 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1801 AAAATCATCC CCTACTGAAT CTAAAACGCG TTAAGTCgac aatcaacctc tggattacaa 1861 aatttgtgaa agatt